Results 271 to 280 of about 556,937 (315)
Some of the next articles are maybe not open access.
Growth of antibody-producing cell clones in microcultures
1987Publisher Summary This chapter describes growth of antibody-producing cell clones in microcultures. The objective of the microculture technique is to analyze the frequency of rare cells involved in the immune response. The precursors of antibody-forming cells (B cells), helper T cells, and suppressor T cells are considered rare cells.
openaire +2 more sources
Separation of mitogen-induced suppressor cells of human antibody-producing cells
Cellular Immunology, 1980Abstract Human antibody-forming cells were demonstrated by a plaque in agar technique following in vitro stimulation with either pokeweed mitogen or Cowan I strain of protein A-positive Staphylococcus aureus bacteria. We evaluated the effects on this antibody formation caused by the addition of cells which had been stimulated with PH A or Con A. Both
J T, Kurnick, F, Pandolfi, H, Wigzell
openaire +2 more sources
Low number of antibody producing cells in patients with sickle cell anemia
Immunology Letters, 1992B cell function is impaired in patients with sickle cell anemia. Although the number of surface IgM positive cells was similar in sickle cell patients and controls, in vitro spontaneous IgM, and PWM stimulated IgA, IgM, and IgG synthesis was significantly lower in the patients than in controls.
N, Rautonen +5 more
openaire +2 more sources
Effect of antibiotics on the development of antibody-producing cells, in vitro
Biochemical Pharmacology, 1972Abstract Using the Mishell-Dutton technique † for culturing mouse spleen cells to produce hemolysin plaque-forming cells (PFC) in vitro , actinomycin D, puromycin or cycloheximide were added daily to determine antibiotic-sensitive sites. The culture period was functionally divided into three temporal phases: (a) An initial phase (0–24 hr) of ...
openaire +2 more sources
Toxin–antigen conjugates as selection tools for antibody producing cells
Journal of Immunological Methods, 2013The generation of antibodies with designated specificity requires cost-intensive and time-consuming screening procedures. Here we present a new method by which hybridoma cells can be selected based on the specificity of the produced antibody by the use of antigen-toxin-conjugates thus eliminating the need of a screening procedure.
Messerschmidt, Katrin, Heilmann, Katja
openaire +2 more sources
[Heterogeneity of antibodies produced by individual cells].
Annales d'immunologie, 1977Recent experimental findings indicate that individual B cells can produce simultaneously heterogeneous Ig molecules. Three orders of heterogeneity have been described. (1) In two published cases of human myeloma, individual malignant cells produced Igkappa and Iglambda (case Im) or IgMlambda and IgGkappa (case Br).
P, Liacopoulos, J, Couderc
openaire +1 more source
A New Method for the Enumeration of Single Antibody-Producing Cells
The Journal of Immunology, 1968Summary A new method is described for the enumeration of single cells producing antibody against a pure protein preparation from Salmonella adelaide flagella. Cells with adherent bacteria, cultured in nutrient agar, give rise to bacteria adherence colonies which can be counted under low power magnification.
openaire +2 more sources
Electron Micrography of Antibody-Producing Cells
Nature, 1965A E, BUSSARD, J L, BINET
openaire +2 more sources
Transplantation of antibody producing cells or their precursors by parabiosis
Life Sciences, 1963Abstract Evidence is presented for the transplantation of an immunologically conditioned cell by parabiosis. The transplantation of such a cell appears not to be related to irradiation of the non-immunized member of the pair.
openaire +1 more source
Human:Mouse Antibody-Producing Transfectoma Cell Lines
1985A new means to generate antibodies (Ab’s) with desired antigen-specificities and biological effector functions is to create transfectoma Ab-producing cell lines. These cell lines are derived from lymphoid cell lines that have been transfected with novel immunoglobulin genes using DNA-mediated gene transfer techniques.
Vernon T. Oi, Sherie L. Morrison
openaire +1 more source