Results 121 to 130 of about 1,117,835 (218)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Clinical Relevance and Methodological Comparison of Anti-MDA5 Antibody Detection: A Five-Year Retrospective and Exploratory Pilot Study. [PDF]

Biomolecules
Mogyoróssy S, Nagy G, Griger Z, Nagy-Vincze M, Bodoki M, Csige D, Tarr T, Zöld É, Pfliegler G, Brúgós BC, Lu H, Tansley S, Antal-Szalmás P, Domján A, Szamosi S, Szűcs G, Szekanecz Z, Horváth Á, Bodoki L.   +18 more
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Diagnostic Performance of a Multiantigen Print ImmunoAssay (MAPIA) for Antibody Detection in Human Neurocysticercosis. [PDF]

Open Forum Infect Dis
Toribio LM, Guzman C, Vasquez A, Saavedra H, Gonzales I, Bustos JA, García HH.   +6 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Development of an indirect ELISA based on a multi-epitope recombinant protein for antibody detection against African swine fever virus. [PDF]

Microbiol Spectr
Liu L, Chen Q, Yu L, Wu W, Mou S, Guo H, Tan C, Liu Q, Wang D, Chen H, Wang X.   +10 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Diagnostic potential for IgM antibody detection by the DPP Syphilis TnT assay in neonates at risk for congenital syphilis. [PDF]

J Clin Microbiol
Stafford IA, Ugartemendia Ugalde L, Goetzl LM, Mosqueda A, DaCosta S, Gunasekera D, Rivieccio M, Esfandiari J, Lyashchenko KP.   +8 more
europepmc   +1 more source

One size does not fit all: An in vitro evaluation of the effects of bezafibrate and medroxyprogesterone acetate on human SH‐SY5Y and U‐87 MG cancer cells

FEBS Open Bio, EarlyView.
Drugs previously repurposed to target blood cancers reduced neuroblastoma and glioblastoma cell growth and viability. However, their levels of anticancer activity were different and their clinical application may be problematic due to side effects at effective doses.
Abhishek Kharawatkar, Francesco Michelangeli, Farhat Latif Khanim, Christopher Martin Bunce, William Eustace Basil Johnson   +4 more
wiley   +1 more source

Standardizing pre-existing anti-PEG antibody detection: A key to clarifying their binding features and clinical relevance. [PDF]

J Pharm Anal
Fu J, Ding T, Li G, Wu E, Wei A, Liu M, Wang B, Zhan C.   +7 more
europepmc   +1 more source