Results 21 to 30 of about 1,117,835 (218)

Establishment of a Dual-Antigen Indirect ELISA Based on p30 and pB602L to Detect Antibodies against African Swine Fever Virus

Viruses, 2023
African swine fever (ASF) is an acute, virulent, and highly fatal infectious disease caused by the African swine fever virus (ASFV). There is no effective vaccine or diagnostic method to prevent and control this disease currently, which highlights the ...
Lei Zhou, Jinxing Song, Mengxiang Wang, Zhuoya Sun, Junru Sun, Panpan Tian, Guoqing Zhuang, Angke Zhang, Yanan Wu, Gaiping Zhang   +9 more
doaj   +1 more source

Development and Evaluation of a Monoclonal Antibody-Based Blocking Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies against Novel Duck Reovirus in Waterfowl Species

Microbiology Spectrum, 2022
The novel duck reovirus (NDRV) is an emerging pathogen that causes disease in various waterfowl species. Since the outbreak, it has caused huge economic losses to the duck industry in China.
Tao Yun, Jionggang Hua, Weicheng Ye, Liu Chen, Zheng Ni, Yinchu Zhu, Cun Zhang   +6 more
doaj   +1 more source

I329L protein-based indirect ELISA for detecting antibodies specific to African swine fever virus

Frontiers in Cellular and Infection Microbiology, 2023
African swine fever (ASF) is a disease that causes severe economic losses to the global porcine industry. As no vaccine or drug has been discovered for the prevention and control of ASF virus (ASFV), accurate diagnosis and timely eradication of infected ...
Zhiyong Shen, Zhiyong Shen, Wenchen Qiu, Haorui Luan, Chunxi Sun, Xinya Cao, Gang Wang, Gang Wang, Jun Peng, Jun Peng   +9 more
doaj   +1 more source

A Customized Novel Blocking ELISA for Detection of Bat-Origin Swine Acute Diarrhea Syndrome Coronavirus Infection

Microbiology Spectrum, 2023
Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a newly discovered emerging alphacoronavirus. SADS-CoV shares over 90% genome sequence identity with bat alphacoronavirus HKU2.
Liyan Cao, Xiangyu Kong, Xiangtong Li, Xuepeng Suo, Yueyue Duan, Cong Yuan, Yu Zhang, Haixue Zheng, Qi Wang   +8 more
doaj   +1 more source

Identification of a novel B-cell epitope of the African swine fever virus p34 protein and development of an indirect ELISA for the detection of serum antibodies

Frontiers in Microbiology
African swine fever (ASF) is a viral disease caused by the African swine fever virus that can be highly transmitted and lethal in domestic pigs. In the absence of a vaccine, effective diagnosis is critical for minimizing the virus’s spread.
Yuanyuan Tian, Yuanyuan Tian, Chao Liang, Chao Liang, Jingming Zhou, Jingming Zhou, Fanglin Sun, Fanglin Sun, Yankai Liu, Yankai Liu, Yumei Chen, Yumei Chen, Xifang Zhu, Xifang Zhu, Hongliang Liu, Hongliang Liu, Peiyang Ding, Peiyang Ding, Enping Liu, Enping Liu, Ying Zhang, Ying Zhang, Sixuan Wu, Sixuan Wu, Aiping Wang, Aiping Wang, Aiping Wang   +26 more
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Review of Infections With Bovine Herpesvirus 1 in Slovenia

Frontiers in Veterinary Science, 2021
In the 1950s, infectious bovine rhinotracheitis/infectious pustular vulvovaginitis (IBR/IPV) disease was clinically detected and documented in cattle for the first time in Slovenia.
Peter Hostnik, Danijela Černe, Janko Mrkun, Jože Starič, Ivan Toplak   +4 more
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source