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Antibodies to protozoan variable surface antigens induce antigenic variation

2022
AbstractThe genomes of most protozoa encode families of variant surface antigens, whose mutually exclusive changes in expression allow parasitic microorganisms to evade the host immune response1,2. It is widely assumed that antigenic variation in protozoan parasites is accomplished by the spontaneous appearance within the population of cells expressing
Albano H. Tenaglia   +17 more
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Vertebrate Cells Express Protozoan Antigen After Hybridization

Science, 1980
Epimastigotes, the invertebrate host stage of Trypanosoma cruzi , the protozoan parasite causing Chagas' disease in man, were fused with vertebrate cells by using polyethylene glycol. Hybrid cells were selected on the basis of T.
M S, Crane, J A, Dvorak
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ANTIBODY‐INDUCED MOVEMENT OF PROTOZOAN SURFACE MEMBRANE ANTIGENS*

Annals of the New York Academy of Sciences, 1975
Direct and indirect immunofluorescence techniques were used to study the effects of host antibodies on surface membrane antigens of viable Leishmania parasites in vitro. Antisera to L. enriettii and L. tropica caused surface membrane antigens of these parasites to aggregate, move toward the poles of the parasite, and to eventually disappear.
J J, Doyle   +3 more
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Distribution of a centrosomal antigen during morphogenesis in the ciliated protozoan Euplotes

Biology of the Cell, 1998
Ciliates assemble basal bodies in great number at many stages of the life‐cycle. In order to understand their assembly mechanisms, we screened a library of monoclonal antibodies directed against pericentriolar material. One of these antibodies, CTR210, was used previously to follow steps of this assembly process: in Paraurostyla, new basal bodies ...
A, Fleury, M, Lemullois, G, Coffe
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Molecular Characterization of a Protozoan Parasite Target Antigen Recognized by Nonspecific Cytotoxic Cells

Cellular Immunology, 1997
The target cell antigen(s) on tumor cells and on protozoan parasites recognized by NK and nonspecific cytotoxic cells (NCC) has not yet been specifically identified. NCC may be the teleost equivalent of NK cells and IL-2-activated NK cells. A ligand recognized by NCC has been identified.
L, Jaso-Friedmann   +4 more
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Expression of protozoan antigens by hybrids between Leishmania mexicana and myeloma SP2/0

Journal of Immunological Methods, 1984
Hybrid cells were obtained between Leishmania mexicana promastigotes and mouse myeloma SP2/0 cells, and examined for expression of leishmanial antigens. A ratio of 1:10 of myeloma to T. cruzi cells was unsuccessful because of outgrowth of non-fused cells.
H, Tomina, A J, Marcipar, G B, Broun
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Detection of chicken antibody to the protozoan parasite Eimeria tenella by antigen-coated microspheres

Journal of Immunological Methods, 1986
A sensitive immunoassay for detecting antibodies to the protozoan parasite of poultry Eimeria tenella, has been developed. Microspheres coated with parasite antigen are reacted with antibody-containing chicken serum followed by a fluorescent labeled second antibody and analyzed by flow cytometry.
B A, Zemcik, P K, Murray, T T, MacDonald
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Common Antigenic Determinants between Acetylcholine Receptor and Protozoan Membranes

1986
It has been reported that sera from humans infected with the protozoan Leishmania donovani contain antibodies to skeletal muscle constituents. In an attempt to find antigenic similarities between specific muscle antigens and Leishmania membrane antigens which could explain the crossreactivity observed we used a library of well characterized monoclonal ...
K. Soteriadou, A. Tzinia, S. Tzartos
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A facile and novel route to the antigenic branched phosphoglycan of the protozoan Leishmania major parasite

Tetrahedron Letters, 2004
Abstract A novel approach for the iterative synthesis of the antigenic branched phosphoglycan of the protozoan parasite Leishmania major is presented that exploits remarkable dual selectivity in the functionalization of d -lactal, providing flexibility to extend the PG chain either towards the reducing or nonreducing end.
Dipali Ruhela, Ram A. Vishwakarma
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