Results 71 to 80 of about 83,454 (234)

High‐Throughput Generation of Tumor Spheroids via Droplet Microfluidics for siRNA‐Loaded Nanomedicine Assessment

Advanced Healthcare Materials, EarlyView.
A droplet microfluidic platform is employed to enable high‐throughput, uniform tumor spheroid generation for evaluating siRNA‐loaded nanomedicines at the protein level. As a proof of concept, breast (MCF‐7) and brain (U87 MG) cancer cell lines are investigated using this platform, revealing penetration profiles and therapeutic responses between the two
Ling Liu, Guoying Wang, Yang Zhang, Bingyang Shi, Ming Li   +4 more
wiley   +1 more source

Polymeric Gene Delivery for Diabetic Treatment [PDF]

Diabetes & Metabolism Journal, 2011
Several polymers were used to delivery genes to diabetic animals. Polyaminobutyl glycolic acid was utilized to deliver IL-10 plasmid DNA to prevent autoimmune insulitis of non-obese diabetic (NOD) mouse.
Sung Wan Kim
doaj   +1 more source

Cationic porphyrins: novel delivery vehicles for antisense oligodeoxynucleotides [PDF]

Nucleic Acids Research, 1998
Cationic porphyrins form stable complexes with oligodeoxynucleotides. To evaluate delivery, we used a 20mer phosphorothioate oligomer (Isis 3521) targeted to the 3'-untranslated region of the PKC-alpha mRNA, and complexed it with porphyrin. The expression of PKC-alpha protein and mRNA in T24 bladder carcinoma cells was reduced by approximately 80 ...
L, Benimetskaya, G B, Takle, M, Vilenchik, I, Lebedeva, P, Miller, C A, Stein   +5 more
openaire   +2 more sources

Targeting the ARRDC3–DRP1 Axis via hUMSC‐Derived Exosomal CRYAB for Neuroprotection in Cerebral Ischemia/Reperfusion Injury

Advanced Healthcare Materials, EarlyView.
Intranasally administered hUMSC‐derived exosomes modulate the CRYAB–ARRDC3–Drp1 axis, alleviating mitochondrial dysfunction and ferroptosis, enhancing neuronal survival, reducing oxidative stress, and promoting functional recovery in ischemia‐reperfusion injury, offering a promising therapeutic strategy for ischemic stroke.
Rong ji, Zengyu Zhang, Zhuohang Liu, Kaicheng Yang, Xueyu Mao, Min Chu, Yong Wang, Jing Zhao   +7 more
wiley   +1 more source

siRNA Delivery via Cross‐Linked Gelatin Microparticles Enables Targeted Modulation of Osteogenic‐Vascular Cross‐Talk: An Advanced Human 3D in Vitro Test System for Therapeutic siRNA

Advanced Healthcare Materials, EarlyView.
Osteogenic‐angiogenic cross‐talk is a vital prerequisite for vascularized bone regeneration. In this study, we investigated the effects of siRNA‐mediated silencing of two inhibitory proteins, Chordin and WWP‐1, via CaP‐NP‐loaded gelatin microparticles in osteogenically differentiated microtissues.
Franziska Mitrach, Jonas Kubat, Stefan Simm, Alexandra H. Springwald, Burak Demir, Anton Liebezeit, Michael C. Hacker, Michaela Schulz‐Siegmund   +7 more
wiley   +1 more source

An Investigation of Innate Immune Response of Human Blood Macrophage to Sense and Antisense dsRNA

Trends in Peptide and Protein Sciences, 2016
Silencing of gene expression by siRNA (small interfering RNA) is a powerful approach used to study the genetic analysis and functional roles of mammalian genes.
Nasrin Mohajeri, Zarrin Sharifnia, Mojgan Bandehpour, Fatemeh Yarian, Amaneh Koochaki, Nariman Mosaffa, Kazem Parivar, Bahram Kazemi   +7 more
doaj   +1 more source

From RNA to DNA: How Cargo Identity Reprograms Lipid Nanoparticle Architecture and Function

Advanced Healthcare Materials, EarlyView.
The evolution of lipid nanoparticles (LNPs) spans from RNA‐LNPs, used in mRNA vaccines, to DNA‐LNPs, ideal for gene therapies. Emerging bionano architectures, decorated with DNA and plasma proteins, pave the way for advanced DNA‐based therapies that are more stable, targeted, and customizable.
Erica Quagliarini, Daniela Pozzi, Giulio Caracciolo   +2 more
wiley   +1 more source

Efficient Delivery of Antisense Oligonucleotides Using Bioreducible Lipid Nanoparticles In Vitro and In Vivo

Molecular Therapy: Nucleic Acids, 2020
The efficient delivery of antisense oligonucleotides (ASOs) to the targeted cells and organs remains a challenge, in particular, in vivo. Here, we investigated the ability of a library of biodegradable lipid nanoparticles (LNPs) in delivering ASO to both
Liu Yang, Feihe Ma, Fang Liu, Jinjin Chen, Xuewei Zhao, Qiaobing Xu   +5 more
doaj   +1 more source

Synthesis and anti-HIV activity of thiocholesteryl-coupled phosphodiester antisense oligonucleotides incorporated into immunoliposomes [PDF]

, 1994
Encapsulation of oligonucleotides in antibody-targeted liposomes (immunoliposomes) which bind to target cells permits intracellular delivery of the oligonucleotides.
Akhtar, Akhtar, Barré-Sinoussi, Boutorine, Boutorine, Boutorine, Boutorine, de Smidt, Feener, Gamper, Goodchild, Hope, Kabanov, Kim, Kinchington, Krieg, Lemke, Leonetti, Leserman, Leserman, Letsinger, Loke, Machy, Machy, Machy, Matsukura, Matthay, Oberhauser, Rebaï, Rey, Saison-Behmoaras, Senior, Shaw, Shea, Stein, Stewart, Suzuki, Svinarchuk, Thierry, Uhlmann, Yakubov, Zamecnik, Zelphati   +42 more
core   +1 more source

Nanotherapies for Atherosclerosis: Targeting, Catalysis, and Energy Transduction

Advanced Healthcare Materials, EarlyView.
Atherosclerosis management is hindered by poor drug targeting and plaque heterogeneity. Nanotechnology overcomes these barriers via three core strategies: (1) target‐engineered nanocarriers that achieve lesion‐specific precision via ligand modification, biomimetic camouflage, stimuli‐responsive release, and self‐propelling nanomotors; (2) catalytic ...
Yuqi Yang, Jingguo Qu, Tonghuan Xiao, Xinyang Han, Michael Tin‐Cheung Ying   +4 more
wiley   +1 more source