Results 301 to 310 of about 124,170 (327)
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Antisense Oligonucleotides: Promise and Reality
Annual Review of Pharmacology and Toxicology, 2001Antisense oligonucleotides have been used for more than a decade to downregulate gene expression. Phosphodiester oligonucleotides are nuclease sensitive, and the more nuclease-resistant phosphorothioate oligonucleotides are now in common use in the laboratory and have entered clinical trials.
Cy A. Stein, Irina V. Lebedeva
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Seminars in cancer biology, 1992
Growing evidence indicates that antisense oligodeoxynucleotides can specifically inhibit gene expression thereby providing an essential tool for understanding gene function and the potential to affect abnormal cell proliferation. Because oncogene activation is intimately involved in tumour initiation and progression, down-regulation of oncogene ...
CALABRETTA, Bruno, Skorski T, Zon G.
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Growing evidence indicates that antisense oligodeoxynucleotides can specifically inhibit gene expression thereby providing an essential tool for understanding gene function and the potential to affect abnormal cell proliferation. Because oncogene activation is intimately involved in tumour initiation and progression, down-regulation of oncogene ...
CALABRETTA, Bruno, Skorski T, Zon G.
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History of Antisense Oligonucleotides
2003Biological science is a rapidly flowing experimental stream, at times encountering a dam that impedes further progress. At such a pomt, a single crack may induce a major breakthrough Discovery of the double helical structure of DNA in 1953 (1) caused such an event, with flooding of new information into the area now known as molecular biology.
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2006
Publisher Summary Antisense oligonucleotides (ASO) offer significant advantages over traditional methods in terms of specificity and versatility. ASOs validate the function of genes in vitro and in vivo. In addition, antisense oligonucleotides are developed as drugs with many products currently being tested in clinical trials. ASOs are short stretches
Erich Koller, Nicholas M. Dean
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Publisher Summary Antisense oligonucleotides (ASO) offer significant advantages over traditional methods in terms of specificity and versatility. ASOs validate the function of genes in vitro and in vivo. In addition, antisense oligonucleotides are developed as drugs with many products currently being tested in clinical trials. ASOs are short stretches
Erich Koller, Nicholas M. Dean
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Antisense Anticancer Oligonucleotide Therapeutics
Current Cancer Drug Targets, 2001Recent progress made in molecular biology, biotechnology, and genetics, especially in identifying, cloning, sequencing and characterization of normal and pathogenic genes, has led to the development of genetic therapy. Major efforts in the field can be summarized in two general approaches: gene therapy and antisense therapy. The second is to deliver to
Gautam Prasad +3 more
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Progress in Antisense Oligonucleotide Therapeutics
Annual Review of Pharmacology and Toxicology, 1996The past several years have seen substantial progress in the development of antisense oligonucleotides as pharmacological tools and as therapeutic agents. With properly designed and executed experiments, it has been possible to demonstrate selective inhibition of gene expression, owing to an antisense mechanisms of action both in cell culture-based ...
Stanley T. Crooke, C F Bennett
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Antisense Oligonucleotide Therapy in Urology
Journal of Urology, 2002Antisense oligonucleotides are short modified DNA or RNA molecules designed to bind selectively messenger RNA and inhibit synthesis of the encoded protein. In the last 20 years antisense technology has emerged as an exciting and promising strategy, especially for treating cancer.
Ingo Kausch, A. Böhle
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Mini-antisense Oligonucleotides
Nucleosides and Nucleotides, 1997Abstract A new strategy of selective DNA target modification was proposed. The using of reactive derivatives of short oligonucleotides in the presence of flanking effector pair allows one to modify DNA target only when the perfect complementary complex of DNA target and oligonucleotide tandem is formed.
Dmitrii V. Pyshnyi +4 more
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Specificity of antisense oligonucleotides
Perspectives in Drug Discovery and Design, 1996Antisense oligodeoxynucleotides that are sufficiently long to specify unique species of mRNA may direct ribonuclease H (RNase H) to cleave nontargeted mRNAs at sites of partial complementarity, both in cell-free systems and in living cells. Specificity of antisense action against selected gene expression may be achieved by increasing the stringency of ...
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Conjugated Antisense Oligonucleotides
Nucleosides and Nucleotides, 1997Abstract We have employed chemical modification strategies to improve cellular ahsorption of oligonucleotides. These include the conjugation of various pendant moieties to the oligonucleotide to affect its overall physical properties such as hydrophobicity, charge, and amphipathicity as well as pendants that may mediate absorption by binding to certain
Isabelle Barber-Peoc'h +11 more
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