Results 131 to 140 of about 2,793 (153)
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Electrofocusing Structural and Induced Proteins of Aphthovirus
Current Topics in Microbiology and Immunology, 1983Electrofocusing separates ampholytes such as proteins according to their isoelectric point. Different versions of the technique have been described under various unwieldy names such as isoelectric focusing or non-equilibrium pH-gradient electrophoresis, but we prefer to apply the term electrofocusing to any type of pH-gradient electrophoresis.
J W Newman, J R Crowther, D Mccahon
exaly +3 more sources
Biochemical evidence of recombination within the unsegmented RNA genome of aphthovirus
Journal of Virology, 1982Four different pairs of temperature-sensitive mutants, derived from the same strain of aphthovirus, were crossed by using an infectious center recombination test. Each parental mutant carried an unselected marker affecting the isoelectric point of a virus-coded polypeptide; progeny of the crosses, able to grow at the nonpermissive temperature, were ...
J W Newman, D Mccahon, W R Slade
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Virology, 1987
We have compared several aspects of an aphthovirus strain attenuated for cattle (C3R-O/E) with the original strain (C3Res) from which it was derived after serial passages in chicken embryos. Biochemical differences detected by protein analysis in regular polyacrylamide gels (SDS-PAGE) and on electrofocusing gels (NEPHGE) suggest the presence of ...
Íngrid E Bergmann +2 more
exaly +3 more sources
We have compared several aspects of an aphthovirus strain attenuated for cattle (C3R-O/E) with the original strain (C3Res) from which it was derived after serial passages in chicken embryos. Biochemical differences detected by protein analysis in regular polyacrylamide gels (SDS-PAGE) and on electrofocusing gels (NEPHGE) suggest the presence of ...
Íngrid E Bergmann +2 more
exaly +3 more sources
Virology, 1986
Monolayers of BHK cells infected with aphthovirus (FMDV) were labeled for short times with [35S]methionine at 2 hr p.i. and fractionated by detergent treatment and low speed centrifugation. Polyacrylamide gel analysis showed an asymmetric distribution of the FMDV-induced polypeptides among the three different subcellular fractions obtained. Polypeptide
P R, Grigera, A, Sagedahl
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Monolayers of BHK cells infected with aphthovirus (FMDV) were labeled for short times with [35S]methionine at 2 hr p.i. and fractionated by detergent treatment and low speed centrifugation. Polyacrylamide gel analysis showed an asymmetric distribution of the FMDV-induced polypeptides among the three different subcellular fractions obtained. Polypeptide
P R, Grigera, A, Sagedahl
exaly +3 more sources
Microbial Pathogenesis, 2019
Foot and Mouth disease (FMD) is economically devastating, highly contagious transboundry viral disease of livestock with 100% morbidity, rapid spread and severe production losses in animals. The FMDV has seven different serotypes. There is no vaccine that can protect animals from all serotypes.
Masood Rabbani, Tahir Yaqub
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Foot and Mouth disease (FMD) is economically devastating, highly contagious transboundry viral disease of livestock with 100% morbidity, rapid spread and severe production losses in animals. The FMDV has seven different serotypes. There is no vaccine that can protect animals from all serotypes.
Masood Rabbani, Tahir Yaqub
exaly +3 more sources
Virology, 1988
A sample of aphthovirus type C3 strain Resende carrying two polyribocytidilic acid [poly(C)] tracts was cloned in tissue culture. One clone with a poly(C)-rich tract of about 145 nucleotides long (clone 3B) and another with a poly(C)-rich tract of about 230 nucleotides long (clone 12) and a mixture of both were injected intralingually into three steers.
I E Bergmann, J L la Torre, I Gomes
exaly +3 more sources
A sample of aphthovirus type C3 strain Resende carrying two polyribocytidilic acid [poly(C)] tracts was cloned in tissue culture. One clone with a poly(C)-rich tract of about 145 nucleotides long (clone 3B) and another with a poly(C)-rich tract of about 230 nucleotides long (clone 12) and a mixture of both were injected intralingually into three steers.
I E Bergmann, J L la Torre, I Gomes
exaly +3 more sources
Persistence and chronic urinary shedding of the aphthovirus equine rhinitis A virus
Journal of Equine Veterinary Science, 2012Equine rhinitis A virus (ERAV) is a member of the Aphthovirus genus, and has many physical and structural similarities to the prototype Aphthovirus foot-and-mouth disease virus (FMDV). The pathogenesis of FMDV has been extensively studied, however, the similarities in the pathogenesis of ERAV and FMDV disease has not been well documented.
Stacey E, Lynch +4 more
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Gene, 1998
Initiation of translation in picornavirus RNAs occurs internally, mediated by an internal ribosome entry site (IRES) element. This property has been exploited to coexpress proteins from a single bicistronic transcription unit in eukaryotic cells. The region that separates the IRES element from the authentic initiator codon of the second gene plays an ...
S, López de Quinto, E, Martínez-Salas
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Initiation of translation in picornavirus RNAs occurs internally, mediated by an internal ribosome entry site (IRES) element. This property has been exploited to coexpress proteins from a single bicistronic transcription unit in eukaryotic cells. The region that separates the IRES element from the authentic initiator codon of the second gene plays an ...
S, López de Quinto, E, Martínez-Salas
openaire +2 more sources