Results 161 to 170 of about 2,955 (177)

Isolation and characterization of recombinants between attenuated and virulent aphthovirus strains [PDF]

Journal of Virology, 1987
A guanidine-resistant mutant of the attenuated strain of aphthovirus type 01 strain Campos and the original wild-type strain were crossed to generate recombinant viruses. Two independently derived recombinant viruses were isolated. One isolate (RI) contained the P1 (structural proteins) gene region of attenuated strain and P3 (polymerase precursor ...
I E Bergmann, J L la Torre
exaly   +3 more sources

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Biochemical evidence of recombination within the unsegmented RNA genome of aphthovirus

Journal of Virology, 1982
Four different pairs of temperature-sensitive mutants, derived from the same strain of aphthovirus, were crossed by using an infectious center recombination test. Each parental mutant carried an unselected marker affecting the isoelectric point of a virus-coded polypeptide; progeny of the crosses, able to grow at the nonpermissive temperature, were ...
A M KING, D Mccahon, W R Slade
exaly   +3 more sources

Conservation of the secondary structure elements of the 5' untranslated region of cardio- and aphthovirus RNAs

Nucleic Acids Research, 1989
An analysis of published nucleotide sequences of the 5'-untranslated region (5'-UTR) of 7 cardioviruses and 3 aphthoviruses has allowed us to derive a consensus secondary structure model that differs from that previously proposed for the 5'-UTR of entero- and rhinoviruses, though all these viruses belong to the same family, Picornaviridae.
V I Agol, V M Blinov, Vadim I Agol
exaly   +4 more sources

Behavior of intertypic recombinants between virulent and attenuated aphthovirus strains in tissue culture and cattle [PDF]

Journal of Virology, 1988
Two aphthovirus intertypic recombinants between the virulent strain A Venceslau and guanidine-resistant attenuated mutants of either strain C3 Resende or O1 Campos were obtained in an attempt to establish the region(s) of the viral genome responsible for attenuation in cattle. Recombinants that inherited the 3' half of the genome from either attenuated
I Gomes, J L la Torre, I E Bergmann
exaly   +3 more sources

Guanidine-resistant mutants of aphthovirus induce the synthesis of an altered nonstructural polypeptide, P34

Journal of Virology, 1982
Extracts of cells infected with guanidine-resistant mutants of aphthovirus were examined for differences in virus-induced polypeptides by using electrofocusing. Four of 1 independent spontaneous mutants induced the synthesis of an altered nonstructural polypeptide, P34.
A M KING
exaly   +3 more sources

Biochemical characterization of an aphthovirus type C3 strain resende attenuated for cattle by serial passages in chicken embryos

Virology, 1987
We have compared several aspects of an aphthovirus strain attenuated for cattle (C3R-O/E) with the original strain (C3Res) from which it was derived after serial passages in chicken embryos. Biochemical differences detected by protein analysis in regular polyacrylamide gels (SDS-PAGE) and on electrofocusing gels (NEPHGE) suggest the presence of ...
Íngrid E Bergmann, J L la Torre, Eduardo A Scodeller   +2 more
exaly   +3 more sources

Identification of a nucleotide deletion in parts of polypeptide 3A in two independent attenuated aphthovirus strains

Virology, 1990
A set of antisera specific for each viral polypeptide of foot-and-mouth disease virus was used to provide a full comparison of polypeptides of two strains attenuated for cattle with respect to their parental virulent strains. Both attenuated strains, belonging to serotypes O1 Campos and C3 Resende, were obtained through serial passages of the ...
A T, Giraudo, E, Beck, K, Strebel, P A, de Mello, J L, La Torre, E A, Scodeller, I E, Bergmann   +6 more
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Cytoskeletal association of an aphthovirus-induced polypeptide derived from the P3ABC region of the viral polyprotein

Virology, 1986
Monolayers of BHK cells infected with aphthovirus (FMDV) were labeled for short times with [35S]methionine at 2 hr p.i. and fractionated by detergent treatment and low speed centrifugation. Polyacrylamide gel analysis showed an asymmetric distribution of the FMDV-induced polypeptides among the three different subcellular fractions obtained. Polypeptide
P R, Grigera, A, Sagedahl
exaly   +3 more sources

Biochemical characterization of an aphthovirus type 01 strain campos attenuated for cattle by serial passages in chicken embryos

Virology, 1985
The biochemical properties of a virulent and an attenuated strain of foot-and-mouth disease virus (FMDV) Type 0(1) Campos (0(1)C) were compared in order to establish differences that could account for their altered biological functions. The avirulent strain (0(1)C-O/E) was derived from the virulent strain 0(1)C by serial passages in chicken embryos ...
J M, Parisi, P, Costa Giomi, P, Grigera, P, Augé de Mello, I E, Bergmann, J L, La Torre, E A, Scodeller   +6 more
openaire   +2 more sources

Heterogeneity of the polyribocytidilic acid tract in aphthovirus: changes in the size of the poly(C) of viruses recovered from persistently infected cattle

Virology, 1988
A sample of aphthovirus type C3 strain Resende carrying two polyribocytidilic acid [poly(C)] tracts was cloned in tissue culture. One clone with a poly(C)-rich tract of about 145 nucleotides long (clone 3B) and another with a poly(C)-rich tract of about 230 nucleotides long (clone 12) and a mixture of both were injected intralingually into three steers.
I Gomes, I E Bergmann, J L la Torre
exaly   +3 more sources