Results 181 to 190 of about 4,215 (205)

Virus isolate from carp: genetic characterization reveals a novel picornavirus with two aphthovirus 2A-like sequences

open access: closedJournal of General Virology, 2013
Picornaviruses have been isolated from a variety of hosts, mainly mammals and birds. Here, we describe the sequence analysis of carp picornavirus 1 (CPV-1) F37/06 that was isolated from an organ pool (heart, brain, liver) of a common carp (Cyprinus carpio). This carp perished after an accidental discharge of liquid manure into a fish pond and presented
Jeannette Lange   +8 more
openalex   +3 more sources

RNA fingerprinting of South American prototype aphthovirus strains

open access: closedVaccine, 1986
Aphthovirus strains used in South America for vaccine production or as reference for diagnostic purposes were analysed by RNA fingerprinting (RNase T1 maps, one- and two-dimensional gels). The results obtained constitute the basis for a data bank containing available information about the genome structure of strains of aphthovirus prevalent in this ...
P.A De Mello   +6 more
openalex   +3 more sources

Long-range RNA interactions between structural domains of the aphthovirus internal ribosome entry site (IRES)

open access: closedRNA, 1999
Internal initiation of translation is promoted by internal ribosome entry site (IRES) cis-acting elements. Using transcripts that correspond to the structural domains of the foot-and-mouth disease virus (FMDV) IRES, we have identified RNA-RNA interactions between separated domains (1-2, 3, 4-5, or HH) of the IRES structure. All the assayed domains were
Ricardo Ramos   +1 more
openalex   +4 more sources

In silico analysis of four structural proteins of aphthovirus serotypes revealed significant B and T cell epitopes

open access: closedMicrobial Pathogenesis, 2019
Foot and Mouth disease (FMD) is economically devastating, highly contagious transboundry viral disease of livestock with 100% morbidity, rapid spread and severe production losses in animals. The FMDV has seven different serotypes. There is no vaccine that can protect animals from all serotypes.
Sohail Raza   +8 more
openalex   +3 more sources
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Parameters influencing translational efficiency in aphthovirus IRES-based bicistronic expression vectors

Gene, 1998
Initiation of translation in picornavirus RNAs occurs internally, mediated by an internal ribosome entry site (IRES) element. This property has been exploited to coexpress proteins from a single bicistronic transcription unit in eukaryotic cells. The region that separates the IRES element from the authentic initiator codon of the second gene plays an ...
S, López de Quinto, E, Martínez-Salas
openaire   +2 more sources

Identification of a nucleotide deletion in parts of polypeptide 3A in two independent attenuated aphthovirus strains

open access: closedVirology, 1990
A set of antisera specific for each viral polypeptide of foot-and-mouth disease virus was used to provide a full comparison of polypeptides of two strains attenuated for cattle with respect to their parental virulent strains. Both attenuated strains, belonging to serotypes O1 Campos and C3 Resende, were obtained through serial passages of the ...
Ana T. Giraudo   +6 more
openalex   +3 more sources

Analysis of the aphthovirus 2A/2B polyprotein ‘cleavage’ mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal ‘skip’

open access: closedJournal of General Virology, 2001
The 2A region of the aphthovirus foot-and-mouth disease virus (FMDV) polyprotein is only 18 aa long. A ‘primary’ intramolecular polyprotein processing event mediated by 2A occurs at its own C terminus. FMDV 2A activity was studied in artificial polyproteins in which sequences encoding reporter proteins flanked the 2A sequence such that a single, long ...
Michelle Donnelly   +6 more
openalex   +3 more sources

Cytoskeletal association of an aphthovirus-induced polypeptide derived from the P3ABC region of the viral polyprotein

Virology, 1986
Monolayers of BHK cells infected with aphthovirus (FMDV) were labeled for short times with [35S]methionine at 2 hr p.i. and fractionated by detergent treatment and low speed centrifugation. Polyacrylamide gel analysis showed an asymmetric distribution of the FMDV-induced polypeptides among the three different subcellular fractions obtained. Polypeptide
P R, Grigera, A, Sagedahl
openaire   +2 more sources

Long-Term, Large-Population Passage of Aphthovirus Can Generate and Amplify Defective Noninterfering Particles Deleted in the Leader Protease Gene

open access: closedVirology, 1996
During serial undiluted passage of a clonal population of foot-and-mouth disease virus (FMDV C-S8c1) in BHK-21 cells, two species of defective RNA were generated and selected. Sequence analysis revealed that they included deletions within the L-coding region, and retained the correct reading frame for viral protein synthesis.
N. Charpentier   +3 more
openalex   +4 more sources

Heterogeneity of the polyribocytidilic acid tract in aphthovirus: changes in the size of the poly(C) of viruses recovered from persistently infected cattle

open access: closedVirology, 1988
A sample of aphthovirus type C3 strain Resende carrying two polyribocytidilic acid [poly(C)] tracts was cloned in tissue culture. One clone with a poly(C)-rich tract of about 145 nucleotides long (clone 3B) and another with a poly(C)-rich tract of about 230 nucleotides long (clone 12) and a mixture of both were injected intralingually into three steers.
M.P. Costa Giomi   +6 more
openalex   +3 more sources

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