Results 151 to 160 of about 951 (197)
New Chlormequat-Based Ionic Liquids as Plant Resistance Inducers. [PDF]
MoleculesKukawka R +6 more
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Molecular evidence of Apple chlorotic leaf spot virus in Himachal Pradesh
, 2016 Rana, T +5 more
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An improved procedure for the purification of apple chlorotic leaf spot virus
Canadian Journal of Plant Science, 1992 A reproducible procedure is described for the purification of apple chlorotic leaf spot virus (CLSV) from Chenopodium quinoa. Two cycles of sucrose density-gradient centrifugation in a magnesium-containing buffer were used. A relatively high yield of virus particles was obtained with little degradation observed.
Delano James, Paul L. Monette
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Production and use of monoclonal antibodies for the detection of apple chlorotic leaf spot virus
Journal of Virological Methods, 1989 Stable hybridoma cell lines secreting antibodies specific for the apple chlorotic leaf spot virus (CLSV) were produced by fusing spleen cells of a Biozzi mouse immunized with CLSV P863 strain, with the non-secretory P3 X63 Ag8.653 myeloma cell line. Two hybridoma clones producing monoclonal antibodies of the IgG1 subclass were obtained.
Poul, F., Dunez, Jean
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In vitro translation of apple chlorotic leaf spot virus (ACLSV) RNA
Archives of Virology, 1996 The genomic RNA of apple chlorotic leaf spot virus was translated in a rabbit reticulocyte lysate system, yielding a large, 190 K product as well as several other polypeptides of smaller size (60, 56, 22 and 15 kDa). The 22 kDa product was immunoprecipitated by an anti-ACLSV serum and comigrated with purified ACLSV coat protein. In vitro translation of
Candresse, Thierry +3 more
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Archives of Virology, 2016
Apple stem grooving virus (ASGV), apple chlorotic leaf spot virus (ACLSV), and prunus necrotic ringspot virus (PNRSV) were identified in a crab apple tree by small RNA deep sequencing. The complete genome sequence of ACLSV isolate BJ (ACLSV-BJ) was 7554 nucleotides and shared 67.0%-83.0% nucleotide sequence identity with other ACLSV isolates.
Yongqiang Li
exaly +3 more sources
Apple stem grooving virus (ASGV), apple chlorotic leaf spot virus (ACLSV), and prunus necrotic ringspot virus (PNRSV) were identified in a crab apple tree by small RNA deep sequencing. The complete genome sequence of ACLSV isolate BJ (ACLSV-BJ) was 7554 nucleotides and shared 67.0%-83.0% nucleotide sequence identity with other ACLSV isolates.
Yongqiang Li
exaly +3 more sources
Incidence and genetic diversity of apple chlorotic leaf spot virus in Iran
Journal of Plant Pathology, 2019 Apple chlorotic leaf spot virus (ACLSV) is one of the most common viruses infecting apple and pear trees, and Iran is among the top ten apple-producing countries in the world. In the present study, incidence and genetic diversity of ACLSV were investigated in the main apple-growing regions of Iran.
Abtahi F. +5 more
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Some properties of apple chlorotic leaf spot virus and their relation to purification problems
Virology, 1971Abstract The filamentous apple virus, chlorotic leaf spot, seems unusual among elongate plant viruses in several respects. Its ultraviolet absorption characteristics are unusual for a rod-shaped virus. It has a critical, pH-dependent, structural requirement for some divalent cations, and their removal by dialysis, exchange, or chelation causes ...
R M Lister
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Screening of Potential Inhibitor against Coat Protein of Apple Chlorotic Leaf Spot Virus
Cell Biochemistry and Biophysics, 2018In this study, we analyzed Coat protein (CP) of Apple chlorotic leaf spot virus (ACLSV), an important latent virus on Apple. Incidence of the virus is upto 60% in various apple cultivars, affecting yield losses of the order of 10-40% (depending upon the cultivar). CP plays an important role as the sole building block of the viral capsid.
Rituraj Purohit +2 more
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Journal of Plant Biochemistry and Biotechnology, 2011 Expression condition for maximum recovery of recombinant Apple chlorotic leaf spot virus (ACLSV) coat protein was standardized. The in vitro expressed fusion protein with 6xHis tag (~43 Kd) was purified from inclusion bodies and used as an antigen ...
Tanuja Rana +2 more
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