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Virus-induced gene silencing in Prunus fruit and nut tree species by Apple latent spherical virus vectoropenaire +1 more source
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RNA Silencing-Mediated Apple Latent Spherical Virus Vaccine in Plants
Methods in Molecular Biology, 2019The apple latent spherical virus (ALSV), originally isolated from an apple tree in Japan, is a small spherical virus with a diameter of 25 nm and comprises a bisegmented, single-stranded RNA genome (RNA1 and RNA2) and three different capsid proteins (Vp25, Vp20, and Vp24).
Nobuyuki Yoshikawa +2 more
exaly +3 more sources
Journal of Virological Methods, 2022
Crude-sap of apple latent spherical virus (ALSV)-infected Chenopodium quinoa leaves was rub-inoculated on the expanded cotyledons of various Cucurbitaceae plants. Most of the species were systemically infected with the virus without obvious symptoms, except pumpkin (Cucurbita maxima).
Nobuyuki Yoshikawa, Noriko Yamagishi
exaly +3 more sources
Crude-sap of apple latent spherical virus (ALSV)-infected Chenopodium quinoa leaves was rub-inoculated on the expanded cotyledons of various Cucurbitaceae plants. Most of the species were systemically infected with the virus without obvious symptoms, except pumpkin (Cucurbita maxima).
Nobuyuki Yoshikawa, Noriko Yamagishi
exaly +3 more sources
Virus-Induced Gene Silencing in Chili Pepper by Apple Latent Spherical Virus Vector
Journal of Virological Methods, 2019Apple latent spherical virus (ALSV) can infect a variety of crops, usually without inducing symptoms. Partial gene sequences can be introduced into ALSV vectors for the induction of virus-induced gene silencing (VIGS). These features are beneficial for the estimation of gene functions in plants, with relatively concise experimental manipulations. Given
Nobuyuki Yoshikawa +2 more
exaly +3 more sources
Virus Research, 2014 Apple latent spherical virus (ALSV) has small isometric particles that are comprised of two single-stranded RNA species (RNA1 and RNA2) and three capsid proteins (Vp25, Vp20, and Vp24). We constructed ALSV vectors for presenting foreign peptides on the surface of virus particles.
Nobuyuki Yoshikawa
exaly +3 more sources
Seed and pollen transmission of Apple latent spherical virus in apple
Journal of General Plant Pathology, 2010To examine whether Apple latent spherical virus (ALSV) has spread among apple trees in an orchard, we surveyed 21 apple trees surrounding two ALSV-infected trees for virus infection using a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA).
Kazuya Nakamura +5 more
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Archives of Virology, 2007
Apple latent spherical virus (ALSV) expressing green fluorescent protein (GFP-ALSV) was used for analysis of virus-induced gene silencing (VIGS) in tobacco plants expressing GFP (GFP-tobacco). In GFP-tobacco inoculated with GFP-ALSV, small dark spots appeared on inoculated leaves at 5 days post-inoculation (dpi), then expanded, and finally covered the ...
H, Yaegashi +7 more
openaire +2 more sources
Apple latent spherical virus (ALSV) expressing green fluorescent protein (GFP-ALSV) was used for analysis of virus-induced gene silencing (VIGS) in tobacco plants expressing GFP (GFP-tobacco). In GFP-tobacco inoculated with GFP-ALSV, small dark spots appeared on inoculated leaves at 5 days post-inoculation (dpi), then expanded, and finally covered the ...
H, Yaegashi +7 more
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First Report of Apple Latent Spherical Virus Infecting Kiwifruit in China
Plant DiseaseNi Hong, Shaoyong Ke, Kaimei Wang
exaly +2 more sources
Journal of General Plant Pathology, 2015
A new virus affecting currants was detected in the Czech Republic in several cultivars of black, red and white currants and in a currant colonized by the aphid Cryptomyzus ribis. The infection was not associated with any symptoms on the plants. The virus has a divided genome consisting of two single-stranded, polyadenylated RNAs, and partial sequences ...
Karel Petrzik +3 more
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A new virus affecting currants was detected in the Czech Republic in several cultivars of black, red and white currants and in a currant colonized by the aphid Cryptomyzus ribis. The infection was not associated with any symptoms on the plants. The virus has a divided genome consisting of two single-stranded, polyadenylated RNAs, and partial sequences ...
Karel Petrzik +3 more
openaire +1 more source
Virus Genes, 2019
Apple latent spherical virus (ALSV) is a latent virus with wide host range of plant species. In the present study, we prepared ALSV vectors expressing RNA silencing suppressors (RSSs) from eight plant viruses: P19 of carnation Italian ring spot virus (tombusvirus), 2b of peanut stunt virus (cucumovirus), NSs of tomato spotted wilt virus (tospovirus ...
Chunjiang Li +3 more
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Apple latent spherical virus (ALSV) is a latent virus with wide host range of plant species. In the present study, we prepared ALSV vectors expressing RNA silencing suppressors (RSSs) from eight plant viruses: P19 of carnation Italian ring spot virus (tombusvirus), 2b of peanut stunt virus (cucumovirus), NSs of tomato spotted wilt virus (tospovirus ...
Chunjiang Li +3 more
openaire +2 more sources