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Microbiology, 2000
A virus with isometric virus particles (ca. 25 nm) was isolated from an apple tree and named Apple latent spherical virus (ALSV). Virus particles purified from infected Chenopodium quinoa formed two bands with densities of 1·41 and 1·43 g/cm3 in CsCl equilibrium density-gradient centrifugation, indicating that the virus is composed of two components ...
C, Li +5 more
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A virus with isometric virus particles (ca. 25 nm) was isolated from an apple tree and named Apple latent spherical virus (ALSV). Virus particles purified from infected Chenopodium quinoa formed two bands with densities of 1·41 and 1·43 g/cm3 in CsCl equilibrium density-gradient centrifugation, indicating that the virus is composed of two components ...
C, Li +5 more
openaire +2 more sources
Archives of Virology, 2004
Infectious cDNA clones of Apple latent spherical virus (ALSV)-RNA1 (pEALSR1) and -RNA2 (pEALSR2) were constructed using an enhanced 35S promoter. A viral vector was constructed from pEALSR2 by creating artificial protease processing sites by duplicating the Q/G protease cleavage site between 42KP and Vp25. Eight RNA2-derived vectors expressing GFP with
C, Li +3 more
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Infectious cDNA clones of Apple latent spherical virus (ALSV)-RNA1 (pEALSR1) and -RNA2 (pEALSR2) were constructed using an enhanced 35S promoter. A viral vector was constructed from pEALSR2 by creating artificial protease processing sites by duplicating the Q/G protease cleavage site between 42KP and Vp25. Eight RNA2-derived vectors expressing GFP with
C, Li +3 more
openaire +2 more sources
Virus-Induced Gene Silencing of N Gene in Tobacco by Apple Latent Spherical Virus Vectors
2014Virus infections induce an RNA-mediated defense that targets viral RNAs in a nucleotide sequence-specific manner in plants, commonly referred to as virus-induced gene silencing (VIGS). When the virus carries sequences of plant genes, it triggers virus-induced gene silencing (VIGS) and results in the degradation of mRNA of endogenous homologous gene ...
Chunjiang, Li, Nobuyuki, Yoshikawa
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Virus-induced gene silencing in various Prunus species with the Apple latent spherical virus vector
Scientia Horticulturae, 2016Abstract Virus-induced gene silencing (VIGS) has been used as a rapid and effective tool for functional analysis of genes in various plants, including woody fruit tree species. We previously reported the successful induction of VIGS of the endogenous PHYTOENE DESATURASE ( PDS ) gene in apricot ( Prunus armeniaca L.) using Apple latent spherical ...
Takashi Kawai +5 more
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2013
Virus-induced gene silencing (VIGS) is an effective tool for the analysis of the gene function in plants within a short time. However, in woody fruit tree like apple, some of Solanum crops, and soybean, it is generally difficult to inoculate virus vector by conventional inoculation methods.
Noriko, Yamagishi, Nobuyuki, Yoshikawa
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Virus-induced gene silencing (VIGS) is an effective tool for the analysis of the gene function in plants within a short time. However, in woody fruit tree like apple, some of Solanum crops, and soybean, it is generally difficult to inoculate virus vector by conventional inoculation methods.
Noriko, Yamagishi, Nobuyuki, Yoshikawa
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Methods in Molecular Biology, 2015
Virus infection leads to the synthesis of double-stranded RNA during virus replication, and then this infection produces small RNA molecules in the antiviral RNA silencing pathway. Here, we develop an Agrobacterium-mediated inoculation system for ALSV-based vectors.
Tatsuya Kon
exaly +3 more sources
Virus infection leads to the synthesis of double-stranded RNA during virus replication, and then this infection produces small RNA molecules in the antiviral RNA silencing pathway. Here, we develop an Agrobacterium-mediated inoculation system for ALSV-based vectors.
Tatsuya Kon
exaly +3 more sources
Plant Molecular Biology, 2009
Virus-induced gene silencing (VIGS) has great potential as a reverse-genetics tool in plant genomics. In this study, we examined the potential of VIGS in soybean seeds and the emergence stage of soybean plants using Apple latent spherical virus (ALSV) vectors.
Noriko, Yamagishi, Nobuyuki, Yoshikawa
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Virus-induced gene silencing (VIGS) has great potential as a reverse-genetics tool in plant genomics. In this study, we examined the potential of VIGS in soybean seeds and the emergence stage of soybean plants using Apple latent spherical virus (ALSV) vectors.
Noriko, Yamagishi, Nobuyuki, Yoshikawa
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Planta, 2018
Gentian plants ( Gentiana triflora ) severely restrict apple latent spherical virus (ALSV) invasion to the gametes (pollens and ovules) and block seed transmission to progeny plants. Early flowering of horticultural plants can be induced by infection of ALSV vector expressing Flowering Locus T (FT) gene.
Kazuki, Kamada +4 more
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Gentian plants ( Gentiana triflora ) severely restrict apple latent spherical virus (ALSV) invasion to the gametes (pollens and ovules) and block seed transmission to progeny plants. Early flowering of horticultural plants can be induced by infection of ALSV vector expressing Flowering Locus T (FT) gene.
Kazuki, Kamada +4 more
openaire +2 more sources
Planta, 2016
Infection by apple latent spherical virus (ALSV) vectors that promote the expression of Arabidopsis thaliana FLOWERING LOCUS T ( AtFT ) or Gentiana triflora GtFT s accelerates flowering in gentian and lisianthus plants. Apple latent spherical virus (ALSV) has isometric virus particles (25 nm in diameter) that contain two ssRNA species (RNA1 and RNA2 ...
Rym, Fekih +2 more
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Infection by apple latent spherical virus (ALSV) vectors that promote the expression of Arabidopsis thaliana FLOWERING LOCUS T ( AtFT ) or Gentiana triflora GtFT s accelerates flowering in gentian and lisianthus plants. Apple latent spherical virus (ALSV) has isometric virus particles (25 nm in diameter) that contain two ssRNA species (RNA1 and RNA2 ...
Rym, Fekih +2 more
openaire +2 more sources
Journal of General Plant Pathology, 2014
We used reverse transcription quantitative PCR (RT-qPCR) to test for apple latent spherical virus (ALSV) in cotyledons of seeds and true leaves of seedlings from apple trees infected with ALSV to accurately determine the status of infection in seedlings from ALSV-infected trees. The amplification curves of ALSV-RNAs from cotyledons were classified into
Nobuyuki Yoshikawa +2 more
exaly +2 more sources
We used reverse transcription quantitative PCR (RT-qPCR) to test for apple latent spherical virus (ALSV) in cotyledons of seeds and true leaves of seedlings from apple trees infected with ALSV to accurately determine the status of infection in seedlings from ALSV-infected trees. The amplification curves of ALSV-RNAs from cotyledons were classified into
Nobuyuki Yoshikawa +2 more
exaly +2 more sources