Results 111 to 120 of about 169,669 (288)

Applicability of mitotic figure counting by deep learning: a development and pan‐cancer validation study

FEBS Open Bio, EarlyView.
In this study, we developed a deep learning method for mitotic figure counting in H&E‐stained whole‐slide images and evaluated its prognostic impact in 13 external validation cohorts from seven different cancer types. Patients with more mitotic figures per mm2 had significantly worse patient outcome in all the studied cancer types except colorectal ...
Joakim Kalsnes, Maria X. Isaksen, Frida Julbø, Manohar Pradhan, Andreas Kleppe, Sepp De Raedt, Ole‐Johan Skrede, Turid Torheim, John Arne Nesheim, Hans Martin Mohn, Hanne A. Askautrud, Karolina Cyll, Wanja Kildal, Emma Rewcastle, Melinda Lillesand, Vebjørn Kvikstad, Emiel Janssen, Robert Jones, Odd Terje Brustugun, Bjørn Brennhovd, Erik Skaaheim Haug, Lill‐Tove Rasmussen Busund, Elin Richardsen, Sigve Andersen, Tom Dønnem, Kristina Lindemann, Gunnar Kristensen, Neil A. Shepherd, Marco Novelli, Knut Liestøl, David Kerr, Håvard E. Danielsen, Tarjei S. Hveem   +32 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Magnetically actuatable 3D-printed endoscopic microsystems

Communications Engineering
In endoscopy, there is a crucial demand for compact system designs to allow for imaging in narrow spaces and reduce the risk of damage during endoscopic procedures.
Florian Rothermel, Andrea Toulouse, Simon Thiele, Chris Jung, Johannes Drozella, Robert Steinhoff, Harald Giessen, Alois M. Herkommer   +7 more
doaj   +1 more source

Improved Multi-Sensor Fusion Dynamic Odometry Based on Neural Networks

Sensors
High-precision simultaneous localization and mapping (SLAM) in dynamic real-world environments plays a crucial role in autonomous robot navigation, self-driving cars, and drone control. To address this dynamic localization issue, in this paper, a dynamic
Lishu Luo, Fulun Peng, Longhui Dong
doaj   +1 more source

Conception and development of an adaptive optics testbed for free-space optical communication

, 2009
Optical free space communications are an efficient approach to transmit high data-rates with small antennas and low power consumption over large distances. Possible scenarios are links between satellites, up- and down-links from and to ground stations as
Moll, Florian
core  

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Critical review of potential technologies for a wearable benzene sensor system

Sensors and Actuators Reports
We evaluate different methods to detect benzene at a parts-per-billion level regarding their potential to be used in a wearable sensor. Benzene is a carcinogenic molecule, regarded as a major health threat by the World Health Organization.
Tim Dunker, Alain Marc Ferber, Håkon Sagberg, Kari Anne Hestnes Bakke   +3 more
doaj   +1 more source

Extended optimization of chromatic aberrations via a hybrid Taguchi-genetic algorithm for zoom optics with a diffractive optical element

, 2013
[[abstract]]In this research, we propose a new method, a hybrid Taguchi–genetic algorithm (HTGA), for optics and zoom optics with a diffractive optical element (DOE) in order to eliminate chromatic aberration more efficiently than traditional damped ...
劉東官, Fang, Yi Chin;Liu, Tung-Kuan;Tsai, Cheng-Mu;Chou, Jyh-Horng;Lin, Han-Ching;Lin, Wei Teng   +1 more
core   +1 more source