Results 121 to 130 of about 48,526 (265)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Upper and Lower Bounds for Eigenvalues of the Elliptic Operator by Weak Galerkin Quadrilateral Spectral Element Methods

Axioms
In this study, we investigate the upper- and lower-bound approximations of numerical eigenvalues derived by weak Galerkin spectral element methods on arbitrary convex quadrilateral meshes for the Laplace eigenvalue problem.
Xiaofeng Xu, Jiajia Pan
doaj   +1 more source

Approximation Fixpoint Theory with Refined Approximation Spaces

CoRR
Submitted to KR ...
Linde Vanbesien, Bart Bogaerts 0001, Marc Denecker   +2 more
openaire   +2 more sources

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Redundant operators in the exact renormalisation group and in the f(R) approximation to asymptotic safety

, 2013
In this paper we review the definition and properties of redundant operators in the exact renormalisation group. We explain why it is important to require them to be eigenoperators and why generically they appear only as a consequence of symmetries of ...
Morris, Tim R., Dietz, Juergen A.
core   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

The Approximation Characteristics of Weighted Band-Limited Function Space

Mathematics
This article primarily investigates the width problem within weighted band-limited function space in a uniform setting. Through an analysis of the properties of s-numbers, we establish a connection between the widths of weighted band-limited function ...
Yue Li, Guanggui Chen, Yanyan Xu, Xiangyu Pan   +3 more
doaj   +1 more source

On best approximation in L spaces

Journal of Approximation Theory, 1987
Let \(L_ p:=L_ p(S,\Sigma,\mu)\), \(1\leq ...
openaire   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Meshfree Approximation for Multi-Asset Options [PDF]

We price multi-asset options by solving their price partial differential equations using a meshfree approach with radial basis functions under jump-diffusion and geometric Brownian motion frameworks. In the geometric Brownian motion framework, we propose
Aanand Venkatramanan, Emmanuel Hanert
core