Results 61 to 70 of about 105,454 (325)

YoeB toxin is activated during thermal stress. [PDF]

, 2015
Type II toxin-antitoxin (TA) modules are thought to mediate stress-responses by temporarily suppressing protein synthesis while cells redirect transcription to adapt to environmental change.
Garza-Sánchez, Fernando, Hayes, Christopher S, Janssen, Brian D   +2 more
core   +1 more source

A Natural Sweetener‐inducible Genetic Switch Controls Therapeutic Protein Expression in Mammals

Advanced Science, EarlyView.
This study develops a natural sweetener, the psicose‐inducible transgene expression (PURE) system based on an Agrobacterium tumefaciens–derived transcriptional repressor PsiR. The PURE system is highly specific to psicose, being insensitive to other sugars and structurally similar molecules.
Longliang Qiao, Zhihao Wang, Shasha Tang, Yuan Fang, Guiling Yu, Xiaoting Qiu, Lingxue Niu, Tao Yan, Xingwan Liu, Xiaoding Ma, Deqiang Kong, Yang Zhou, Ningzi Guan, Jinzhong Tian, Meiyan Wang, Haifeng Ye, Fengfeng Cai   +16 more
wiley   +1 more source

Identification of Important Amino Acids in Gal2p for Improving the L-arabinose Transport and Metabolism in Saccharomyces cerevisiae

Frontiers in Microbiology, 2017
Efficient and cost-effective bioethanol production from lignocellulosic materials requires co-fermentation of the main hydrolyzed sugars, including glucose, xylose, and L-arabinose.
Chengqiang Wang, Chengqiang Wang, Yanwei Li, Chenxi Qiu, Chenxi Qiu, Shihao Wang, Shihao Wang, Jinjin Ma, Yu Shen, Qingzhu Zhang, Binghai Du, Yanqin Ding, Xiaoming Bao, Xiaoming Bao   +13 more
doaj   +1 more source

Production of Acetoin through Simultaneous Utilization of Glucose, Xylose, and Arabinose by Engineered Bacillus subtilis. [PDF]

PLoS ONE, 2016
Glucose, xylose and arabinose are the three most abundant monosaccharide found in lignocellulosic biomass. Effectively and simultaneously utilization of these sugars by microorganisms for production of the biofuels and bio-chemicals is essential toward ...
Bo Zhang, Xin-Li Li, Jing Fu, Ning Li, Zhiwen Wang, Ya-Jie Tang, Tao Chen   +6 more
doaj   +1 more source

Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia coli strains [PDF]

, 2009
Background: Homologous recombination mediated by the lambda-Red genes is a common method for making chromosomal modifications in Escherichia coli.
Bingle, Lewis EH, Busby, Stephen J. W., Heurlier, Karin, Hobman, Jon L, Lee, David J, Pallen, Mark J., Penn, Charles W   +6 more
core   +5 more sources

SUPER: Upcycling Genetic Parts for Precise Gene Expression Control, Leakage Minimization, and Genetic Circuit Stability

Advanced Science, EarlyView.
To precisely modulate natural and synthetic regulatory parts, SUPER is introduced as a sophisticated and tunable controller of genetic devices without requiring sequence modification on the target. SUPER enhances dynamic range of RNA‐, chemical‐, temperature‐, and protein‐responsive biosensors up to 1011%, and converts biosensors to cover an expanded ...
Taeyang Heo, Dongwon Park, Woosub Shin, Jongmin Kim   +3 more
wiley   +1 more source

The Penicillium chrysogenum transporter PcAraT enables high-affinity, glucose-insensitive l-arabinose transport in Saccharomyces cerevisiae

Biotechnology for Biofuels, 2018
Background l-Arabinose occurs at economically relevant levels in lignocellulosic hydrolysates. Its low-affinity uptake via the Saccharomyces cerevisiae Gal2 galactose transporter is inhibited by d-glucose. Especially at low concentrations of l-arabinose,
Jasmine M. Bracher, Maarten D. Verhoeven, H. Wouter Wisselink, Barbara Crimi, Jeroen G. Nijland, Arnold J. M. Driessen, Paul Klaassen, Antonius J. A. van Maris, Jean-Marc G. Daran, Jack T. Pronk   +9 more
doaj   +1 more source

Identification and structural characterisation of a partially arabinosylated lipoarabinomannan variant isolated from a Corynebacterium glutamicum ubiAmutant [PDF]

, 2007
Arabinan polysaccharide side-chains are present in both Mycobacterium tuberculosis and Corynebacterium glutamicum in the heteropolysaccharide arabinogalactan (AG), and in M. tuberculosis in the lipoglycan, lipoarabinomannan (LAM).
Alderwick, Luke J., Besra, Gurdyal S, Dell, Anne, Eggeling, Lothar, Gilleron, Martine, Giordano, Assunta, Hitchen, Paul, Krumbach, Karin, Mishra, Arun K., Morris, Howard R., Nigou, Jerome, Tatituri, Raju Venkata Veera   +11 more
core  

Probing for Binding Regions of the FtsZ Protein Surface through Site-Directed Insertions: Discovery of Fully Functional FtsZ-Fluorescent Proteins

, 2016
FtsZ, a bacterial tubulin homologue, is a cytoskeletal protein that assembles into protofilaments that are one subunit thick. These protofilaments assemble further to form a “Z ring” at the center of prokaryotic cells. The Z ring generates a constriction
Erickson, Harold P., Joshi, Chandra P., Moore, Desmond A., Osawa, Masaki, Whatley, Zakiya N.   +4 more
core   +2 more sources

DNA Sampling: a method for probing protein binding at specific loci on bacterial chromosomes [PDF]

, 2009
We describe a protocol, DNA sampling, for the rapid isolation of specific segments of DNA, together with bound proteins, from Escherichia coli K-12. The DNA to be sampled is generated as a discrete fragment within cells by the yeast I-SceI meganuclease ...
Blattner, Busby, Butala, Butala, Dame, David J. Lee, Dejardin, Gadgil, Grainger, Grainger, Griffin, Herring, Karp, Kuhar, Lee, Lodge, Matej Butala, Miller, Minchin, Mulec, Murphy, Nordhoff, Pope, Salgado, Spott, Stead, Stephen J. W. Busby, Wang, Westblade, Woo   +29 more
core   +5 more sources