Results 121 to 130 of about 10,969 (168)

Arachidonate 12-lipoxygenases with reference to their selective inhibitors

Biochemical and Biophysical Research Communications, 2005
Lipoxygenase is a dioxygenase recognizing a 1-cis,4-cis-pentadiene of polyunsaturated fatty acids. The enzyme oxygenates various carbon atoms of arachidonic acid as a substrate and produces 5-, 8-, 12- or 15-hydroperoxyeicosatetraenoic acid with a conjugated diene chromophore.
Shozo, Yamamoto, Michiko, Katsukawa, Ayumi, Nakano, Emi, Hiraki, Kohji, Nishimura, Mitsuo, Jisaka, Kazushige, Yokota, Natsuo, Ueda   +7 more
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Diurnal Change of Arachidonate 12-Lipoxygenase in Rat Pineal Gland

Biochemical and Biophysical Research Communications, 1997
Rat pineal gland contains a 12-lipoxygenase as demonstrated by the enzyme activity, RNA blot analysis and in situ hybridization. Using rats maintained with 12-h dark and light cycles, dynamic changes of the enzyme in pineal gland were examined. When the crude extract of pineal glands was incubated with arachidonic acid and the reaction products were ...
H, Kawajiri, N, Qiao, D M, Zhuang, T, Yoshimoto, H, Hagiya, S, Yamamoto, H, Sei, Y, Morita   +7 more
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Eicosanoid regulation of angiogenesis: role of endothelial arachidonate 12-lipoxygenase

Blood, 2000
Angiogenesis, the formation of new capillaries from preexisting blood vessels, is a multistep, highly orchestrated process involving vessel sprouting, endothelial cell migration, proliferation, tube differentiation, and survival. Eicosanoids, arachidonic acid (AA)-derived metabolites, have potent biologic activities on vascular endothelial cells ...
D, Nie, K, Tang, C, Diglio, K V, Honn
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Lipoxin synthesis by arachidonate 12-lipoxygenase purified from porcine leukocytes

Biochemical and Biophysical Research Communications, 1987
Arachidonate 12-lipoxygenase purified from porcine leukocytes shows 14R-oxygenase and 14, 15-leukotriene A synthase activities with 15-hydroperoxy-arachidonic acid as substrate. The enzyme transformed 5, 15-dihydroperoxy-arachidonic acid to several compounds with a conjugated tetraene. A major product was identified as 5S, 14R, 15S-trihydroperoxy-6, 10,
N, Ueda, C, Yokoyama, S, Yamamoto, B J, Fitzsimmons, J, Rokach, J A, Oates, A R, Brash   +6 more
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Gossypol, a potent inhibitor of arachidonate 5- and 12-lipoxygenases

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1985
Gossypol inhibited 5- and 12-lipoxygenases of rat basophilic leukemia (RBL-1) cells with ID50 of 0.3 microM and 0.7 microM, respectively. Nearly two orders of magnitude of higher concentration of gossypol was required to inhibit prostaglandin synthetase. The inhibition was of a non-competitive type with respect to arachidonate.
Y, Hamasaki, H H, Tai
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Arachidonate 12-Lipoxygenase of Human Platelets

1993
Thromboxane A2 is well known as a proaggregatory and vasoconstrictive compound [1]. It is produced from arachidonic acid in platelets, and fatty acid cyclooxygenase responsible for the thromboxane synthesis is localized in the microsomal fraction of human platelets [2]. There is another metabolic pathway of arachidonic acid in human platelets.
Shozo Yamamoto, Satoshi Matsuda, Jun Murakami, Yasuchika Yamamoto, Yumiko Konishi, Chieko Yokoyama, Toshiya Arakawa, Yoshitaka Takahashi, Tanihiro Yoshimoto, Yasuo Mimura, Minoru Okuma   +10 more
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Inhibition of platelet arachidonic acid 12-lipoxygenase by acetylenic acid compounds

Prostaglandins, 1981
Eighteen acetylenic fatty acids were tested as inhibitors of human platelet arachidonic acid 12-lipoxygenase. 4,7,10,13-Eicosatetraynoic (4,7,10,13-ETYA) acid emerged as the most potent compound. Additional experiments have shown that 4,7,10,13-ETYA selectively blocked the 12-lipoxygenase in washed human platelets with lesser activity against the ...
F F, Sun, J C, McGuire, D R, Morton, J E, Pike, H, Sprecher, W H, Kunau   +5 more
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Partial purification and characterization of arachidonate 12-lipoxygenase from rat lung

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1983
Incubation of rat lung cytosol with arachidonic acid produced 12-hydroxy-5,8,10,14-eicosatetraenoic acid as a major product, which was identified by gas chromatography-mass spectrometry. By ammonium sulfate fractionation and DEAE-cellulose chromatography the arachidonate 12-lipoxygenase was purified about 30-fold from the rat lung cytosol.
C, Yokoyama, K, Mizuno, H, Mitachi, T, Yoshimoto, S, Yamamoto, C R, Pace-Asciak   +5 more
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Arachidonate 12-Lipoxygenases: Enzymology and Molecular Biology

1993
In contrast to 5-lipoxygenase which is involved in the biosynthesis of bioactive leukotrienes, a general physiological role of 12-lipoxygenase has not yet been established. Although the enzyme was earlier found in platelets as the first mammalian lipoxygenase and has been found in various tissues of many animal species, most investigators have ignored ...
S. Yamamoto, T. Yoshimoto, N. Ueda, Y. Takahashi, S. Matsuda, H. Suzuki, T. Oshima, H. Ikawa, T. Hada, G. R. Reddy, Y. Yamamoto, K. Kishimoto, T. Arakawa, K. Ishimura, S. Arase, C. Yokoyama, T. Tanabe, H. Toh, M. Nishiyama, H. Okamoto, T. Watanabe, T. Hori   +21 more
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Analysis of non-heme iron in arachidonate 12-lipoxygenase of porcine leukocytes

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1991
Arachidonate 12-lipoxygenase of porcine leukocytes, which was purified to homogeneity by immunoaffinity chromatography, was analyzed for iron content by atomic absorption spectrophotometry. The enzyme contained 0.70 +/- 0.09 g atom of iron per mol of enzyme (mean +/- S.D., n = 4).
S, Matsuda, H, Suzuki, T, Yoshimoto, S, Yamamoto, A, Miyatake   +4 more
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