Results 101 to 110 of about 6,523,049 (268)
Proteins: Structure, Function, and Bioinformatics, EarlyView.ABSTRACT We present the first x‐ray crystallographic structural evidence of an archaeal DNA ligase showing the AMP covalent adduct together with further cofactor hydrolysis, capturing a transient intermediary in the first step of the ligation reaction, triggered by the pyrophosphate hydrolysis.
A. X. Quintana‐Armas +3 more
wiley +1 more source
Biology Direct, 2012 Background In eukaryotes, the CMG (CDC45, MCM, GINS) complex containing the replicative helicase MCM is a key player in DNA replication. Archaeal homologs of the eukaryotic MCM and GINS proteins have been identified but until recently no homolog of the ...
Makarova Kira S +2 more
doaj +1 more source
, 1998 Introns interrupt the continuity of many eukaryal genes, and therefore their removal by splicing is a crucial step in gene expression. Interestingly, even within Eukarya there are at least four splicing mechanisms.
Abelson, John +2 more
core +1 more source
Annals of Applied Biology, EarlyView.A short‐term flood, implemented in a field experiment with sprinkler irrigation in maize production systems, increased soil moisture levels and reduced autoclaved citrate extractable protein, an indicator of soil biological health that reflects organically bound soil nitrogen, and also corresponded to greater differential abundance of soil bacterial ...
Katherine Naasko +7 more
wiley +1 more source
Proposed Role for KaiC-Like ATPases as Major Signal Transduction Hubs in Archaea
mBio, 2017 All organisms must adapt to ever-changing environmental conditions and accordingly have evolved diverse signal transduction systems. In bacteria, the most abundant networks are built around the two-component signal transduction systems that include ...
Kira S. Makarova +2 more
doaj +1 more source
Investigating the molecular mechanisms underlying the anti‐CRISPR function of AcrIIA13b protein
The FEBS Journal, EarlyView.Anti‐CRISPR protein AcrIIA13b inhibits CRISPR–Cas9 function by preventing target DNA engagement. The Cas9–sgRNA ribonucleoprotein (RNP) normally recognizes the protospacer adjacent motif (PAM) duplex to initiate DNA cleavage. AcrIIA13b binding to the PAM‐interacting WED–PI domain blocks this recognition, leaving the target DNA intact.
So Yeon Lee, Hyun Ho Park
wiley +1 more source
Fluorescence-based incision assay for human XPF-ERCC1 activity identifies important elements of DNA junction recognition [PDF]
, 2012 The structure-specific endonuclease activity of the human XPF–ERCC1 complex is essential for a number of DNA processing mechanisms that help to maintain genomic integrity.
Bowles, M. +5 more
core +1 more source
Extreme Deviations from Expected Evolutionary Rates in Archaeal Protein Families [PDF]
Genome Biology and Evolution, 2017 Origin of new biological functions is a complex phenomenon ranging from single-nucleotide substitutions to the gain of new genes via horizontal gene transfer or duplication. Neofunctionalization and subfunctionalization of proteins is often attributed to the emergence of paralogs that are subject to relaxed purifying selection or positive selection and
Petitjean, Celine +3 more
openaire +2 more sources
The FEBS Journal, EarlyView.Zebrafish Tgds, when expressed as a recombinant protein, catalyzes the dehydration of UDP‐D‐glucose, the initial step in the formation of 6‐deoxyhexoses. Corresponding Tgds mutations found in Catel–Manzke syndrome patients lead to reduced enzymatic activity and stability.
Maria Rosaria Coppola +11 more
wiley +1 more source
Archaea, 2010 The recent discovery of protein modification by SAMPs, ubiquitin-like (Ubl) proteins from the archaeon Haloferax volcanii, prompted a comprehensive comparative-genomic analysis of archaeal Ubl protein genes and the genes for enzymes thought to be ...
K. Makarova, E. Koonin
semanticscholar +1 more source