Results 11 to 20 of about 180 (71)

Identification of Genes Involved in the Biosynthesis of the Third and Fourth Sugars of the Methanococcus maripaludis Archaellin N-Linked Tetrasaccharide [PDF]

Journal of Bacteriology, 2013
N-glycosylation is a protein posttranslational modification found in all three domains of life. Many surface proteins in Archaea, including S-layer proteins, pilins, and archaellins (archaeal flagellins) are known to contain N-linked glycans. In Methanococcus maripaludis, the archaellins are modified at multiple sites with an N-linked tetrasaccharide ...
Ding, Y., Jones, G.M., Uchida, K., Aizawa, S.-I., Robotham, A., Logan, S.M., Kelly, J., Jarrell, K.F.   +7 more
openaire   +3 more sources

A way to identify archaellins in Halobacterium salinarum archaella by FLAG-tagging

Open Life Sciences, 2013
Abstract In the current study, haloarchaea Halobacterium salinarum cells were transformed individually with each of the modified archaellin genes (flaA1, flaA2 and flaB2) containing an oligonucleotide insert encoding the FLAG peptide (DYKDDDDK). The insertion site was selected to expose the FLAG peptide on the archaella filament surface.
BEZNOSOV, SN, PYATIBRATOV, MG, VELURI, PS, MITRA, S, FEDOROV, OV   +4 more
openaire   +4 more sources

An archaellum filament composed of two alternating subunits. [PDF]

Nat Commun, 2022
Archaea use a molecular machine, called the archaellum, to swim. The archaellum consists of an ATP-powered intracellular motor that drives the rotation of an extracellular filament composed of multiple copies of proteins named archaellins.
Gambelli L, Isupov MN, Conners R, McLaren M, Bellack A, Gold V, Rachel R, Daum B.   +7 more
europepmc   +10 more sources

SaUspA, the Universal Stress Protein of Sulfolobus acidocaldarius Stimulates the Activity of the PP2A Phosphatase and Is Involved in Growth at High Salinity

Frontiers in Microbiology, 2020
In Sulfolobus acidocaldarius, the protein phosphatase PP2A plays important regulatory roles in many cellular processes, including cell growth, cell shape and synthesis of the archaellum.
Xing Ye, Chris van der Does, Sonja-Verena Albers   +2 more
doaj   +2 more sources

Complementation of an aglB Mutant of Methanococcus maripaludis with Heterologous Oligosaccharyltransferases. [PDF]

PLoS ONE, 2016
The oligosaccharyltransferase is the signature enzyme for N-linked glycosylation in all domains of life. In Archaea, this enzyme termed AglB, is responsible for transferring lipid carrier-linked glycans to select asparagine residues in a variety of ...
Yan Ding, Helen A Vrionis, James Schneider, Alison Berezuk, Cezar M Khursigara, Ken F Jarrell   +5 more
doaj   +2 more sources

Identification of genes involved in biosynthesis of the archaellin N-linked glycan of the archaeon Methanococcus maripaludis [PDF]

, 2014
N-linked glycosylation is a post-translational protein modification that occurs in all three domains of life. Methanococcus maripaludis archaellin proteins are known to be glycosylated by a tetrasaccharide composed of N-acetylgalactosamine, a di-acetylated glucuronic acid, a modified mannuronic acid with an attached threonine, and a novel terminal ...
Siu, Sarah
openaire   +2 more sources

High-resolution archaellum structure reveals a conserved metal-binding site. [PDF]

EMBO Rep, 2019
Many archaea swim by means of archaella. While the archaellum is similar in function to its bacterial counterpart, its structure, composition, and evolution are fundamentally different.
Meshcheryakov VA, Shibata S, Schreiber MT, Villar-Briones A, Jarrell KF, Aizawa SI, Wolf M.   +6 more
europepmc   +3 more sources

Autophosphorylation of the KaiC‐like protein ArlH inhibits oligomerization and interaction with ArlI, the motor ATPase of the archaellum

Molecular Microbiology, Volume 116, Issue 3, Page 943-956, September 2021., 2021
KaiC is the central component in the regulation of the cyanobacterial circadian rhythm. KaiC‐like proteins are widespread in archaea, where they are thought to have originated and thought to perform diverse functions. We show that the KaiC‐like protein ArlH—an essential protein for archaeal motility—possesses autophosphorylation activity which mediates
J. Nuno de Sousa Machado, Leonie Vollmar, Julia Schimpf, Paushali Chaudhury, Rashmi Kumariya, Chris van der Does, Thorsten Hugel, Sonja‐Verena Albers   +7 more
wiley   +1 more source

CryoEM structure of the Methanospirillum hungatei archaellum reveals structural features distinct from the bacterial flagellum and type IV pilus. [PDF]

Nat Microbiol, 2016
Archaea use flagella known as archaella—distinct both in protein composition and structure from bacterial flagella—to drive cell motility, but the structural basis of this function is unknown.
Poweleit N, Ge P, Nguyen HH, Loo RR, Gunsalus RP, Zhou ZH.   +5 more
europepmc   +2 more sources

Evidence that Biosynthesis of the Second and Third Sugars of the Archaellin Tetrasaccharide in the Archaeon Methanococcus maripaludis Occurs by the Same Pathway Used by Pseudomonas aeruginosa To Make a Di-N-Acetylated Sugar [PDF]

Journal of Bacteriology, 2015
ABSTRACTMethanococcus maripaludishas two surface appendages, archaella and type IV pili, which are composed of glycoprotein subunits. Archaellins are modified with an N-linked tetrasaccharide with the structure Sug-1,4-β-ManNAc3NAmA6Thr-1,4-β-GlcNAc3NAcA-1,3-β-GalNAc, where Sug is (5S)-2-acetamido-2,4-dideoxy-5-O-methyl-α-l-erythro-hexos-5-ulo-1,5 ...
Siu, Sarah, Robotham, Anna, Logan, Susan M., Kelly, John F., Uchida, Kaoru, Aizawa, Shin-Ichi, Jarrell, Ken F.   +6 more
openaire   +2 more sources