Reinvestigation of Absorption Spectroscopic Thermal Dynamics of Archaerhodopsin 3 Based Voltage Sensor QuasAr1 [PDF]
BioengineeringThe long-time absorption spectroscopic development of the genetically encoded microbial rhodopsin fluorescent voltage indicator QuasAr1 at room temperature in the dark was reinvestigated, mainly theoretically.
Alfons Penzkofer +2 more
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Photoactivation of Cell-Free Expressed Archaerhodopsin-3 in a Model Cell Membrane. [PDF]
Int J Mol Sci, 2021 Transmembrane receptor proteins are located in the plasma membranes of biological cells where they exert important functions. Archaerhodopsin (Arch) proteins belong to a class of transmembrane receptor proteins called photoreceptors that react to light ...
Khangholi N +4 more
europepmc +7 more sources
Archaerhodopsin Selectively and Reversibly Silences Synaptic Transmission through Altered pH [PDF]
Cell Reports, 2016 Tools that allow acute and selective silencing of synaptic transmission in vivo would be invaluable for understanding the synaptic basis of specific behaviors.
Mohamady El-Gaby +5 more
doaj +5 more sources
Characterization of Channelrhodopsin and Archaerhodopsin in Cholinergic Neurons of Cre-Lox Transgenic Mice. [PDF]
PLoS ONE, 2016 The study of cholinergic signaling in the mammalian CNS has been greatly facilitated by the advent of mouse lines that permit the expression of reporter proteins, such as opsins, in cholinergic neurons. However, the expression of opsins could potentially
Tristan Hedrick +7 more
doaj +6 more sources
Enhanced Archaerhodopsin Fluorescent Protein Voltage Indicators. [PDF]
PLoS ONE, 2013 A longstanding goal in neuroscience has been to develop techniques for imaging the voltage dynamics of genetically defined subsets of neurons. Optical sensors of transmembrane voltage would enhance studies of neural activity in contexts ranging from ...
Yiyang Gong +2 more
doaj +6 more sources
Rational Design of Far-Red Archaerhodopsin-3-Based Fluorescent Genetically Encoded Voltage Indicators: from Elucidation of the Fluorescence Mechanism in Archers to Novel Red-Shifted Variants [PDF]
ACS Physical Chemistry AuGenetically encoded voltage indicators (GEVIs) have found wide applications as molecular tools for visualization of changes in cell membrane potential.
Dmitrii M. Nikolaev +13 more
doaj +4 more sources
Archaerhodopsin 3 is an ideal template for the engineering of highly fluorescent optogenetic reporters. [PDF]
Chem SciArchaerhodopsin-3 (AR-3) variants stand out among other rhodopsins in that they display a weak, but voltage-sensitive, near-infrared fluorescence emission. This has led to their application in optogenetics both in cell cultures and small animals. However,
Herasymenko K +8 more
europepmc +7 more sources
Semirational Protein Engineering Yields Archaerhodopsin-3-Based Fluorescent Genetically Encoded Voltage Indicators with Enhanced Brightness and Red Shifted Absorption Bands [PDF]
Chem & Bio EngineeringA semirational engineering of novel bright variants of archaerhodopsin-based genetically encoded voltage indicators (GEVIs) is performed. The corresponding focused protein sequence library was generated using data derived from prior directed evolution ...
Dmitrii M. Nikolaev +9 more
doaj +5 more sources
Silencing of Cholinergic Basal Forebrain Neurons Using Archaerhodopsin Prolongs Slow-Wave Sleep in Mice. [PDF]
PLoS ONE, 2015 The basal forebrain (BF) plays a crucial role in cortical activation. Our previous study showed that activation of cholinergic BF neurons alone is sufficient to suppress slow-wave sleep (SWS) and promote wakefulness and rapid-eye-movement (REM) sleep ...
Yu-Feng Shi +4 more
doaj +6 more sources
Elucidation of Expression Patterns and Functional Properties of Archaerhodopsin Derived from Halorubrum sp. Ejinoor [PDF]
BiologyThis study elucidates the structural determinants and optogenetic potential of Archaerhodopsin HeAR, a proton pump from Halorubrum sp. Ejinoor isolated from Inner Mongolian salt lakes. Through heterologous expression in E. coli BL21 (DE3) and integrative
Luomeng Chao, Yuxia Yang
doaj +5 more sources