Optogenetic ion pumps differ with respect to the secondary pattern of K+ redistribution [PDF]
Physiological Reports, 2023 We recently reported that strong activation of the optogenetic chloride pump, halorhodopsin leads to a secondary redistribution of K+ ions into the cell, through tonically open, “leak” K+ channels.
R. Ryley Parrish +3 more
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Structures of the archaerhodopsin-3 transporter reveal that disordering of internal water networks underpins receptor sensitization [PDF]
Nature Communications, 2021 Archaerhodopsin-3 (AR3) mutants are commonly used in optogenetics for neuron silencing and membrane voltage sensing. High-resolution crystal structures show that desensitization of the AR3 photoreceptor occurs when internal hydrogen-bonded water networks
Juan F. Bada Juarez +14 more
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Rational Design of Far-Red Archaerhodopsin-3-Based Fluorescent Genetically Encoded Voltage Indicators: from Elucidation of the Fluorescence Mechanism in Archers to Novel Red-Shifted Variants [PDF]
ACS Physical Chemistry AuDmitrii M. Nikolaev +13 more
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A voltage-dependent fluorescent indicator for optogenetic applications, archaerhodopsin-3: Structure and optical properties from in silico modeling [version 2; referees: 1 approved, 2 approved with reservations] [PDF]
F1000Research, 2017 It was demonstrated in recent studies that some rhodopsins can be used in optogenetics as fluorescent indicators of membrane voltage. One of the promising candidates for these applications is archaerhodopsin-3.
Dmitrii M. Nikolaev +4 more
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Archaerhodopsin variants with enhanced voltage-sensitive fluorescence in mammalian and Caenorhabditis elegans neurons [PDF]
, 2014 Probing the neural circuit dynamics underlying behaviour would benefit greatly from improved genetically encoded voltage indicators. The proton pump Archaerhodopsin-3 (Arch), an optogenetic tool commonly used for neuronal inhibition, has been shown to ...
Arnold, Frances H. +8 more
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Semirational Protein Engineering Yields Archaerhodopsin-3-Based Fluorescent Genetically Encoded Voltage Indicators with Enhanced Brightness and Red Shifted Absorption Bands [PDF]
Chem & Bio EngineeringDmitrii M. Nikolaev +9 more
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A high-light sensitivity optical neural silencer: development, and application to optogenetic control of nonhuman primate cortex [PDF]
Frontiers in Systems Neuroscience, 2011 Technologies for silencing the electrical activity of genetically-targeted neurons in the brain are important for assessing the contribution of specific cell types and pathways towards behaviors and pathologies.
Xue eHan +10 more
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Screening fluorescent voltage indicators with spontaneously spiking HEK cells. [PDF]
PLoS ONE, 2013 Development of improved fluorescent voltage indicators is a key challenge in neuroscience, but progress has been hampered by the low throughput of patch-clamp characterization.
Jeehae Park +6 more
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Elucidation of Expression Patterns and Functional Properties of Archaerhodopsin Derived from Halorubrum sp. Ejinoor [PDF]
BiologyThis study elucidates the structural determinants and optogenetic potential of Archaerhodopsin HeAR, a proton pump from Halorubrum sp. Ejinoor isolated from Inner Mongolian salt lakes. Through heterologous expression in E. coli BL21 (DE3) and integrative
Luomeng Chao, Yuxia Yang
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Biology Open, 2013 Summary Optogenetics, the regulation of proteins by light, has revolutionized the study of excitable cells, and generated strong interest in the therapeutic potential of this technology for regulating action potentials in neural and muscle cells. However,
Dany Spencer Adams +2 more
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