Results 121 to 130 of about 9,719,459 (254)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Historical Context and Contemporary Interventions: Integrating AI-Assisted Design Processes Into Architectural Education

open access: yes
Bentley Advancing Infrastructure; POLARKON; TUBITAKThis study explores AI-assisted design methodologies in architectural education through a pedagogical experiment conducted during the 2024-2025 Fall Semester in a third-year architectural design studio ...
Cinar, S., Demiröz, M.
core  

Study on direct displacement-based seismic design method for hinge-connected steel frames with buckling-restrained braces

open access: yesJianzhu Jiegou
Compared with traditional steel frames, the hinge-connected steel frames with buckling-restrained braces exhibit favorable seismic performance, and its connections are less vulnerable to earthquake damage.
WANG Guotao   +7 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Spatial distribution and influencing factors of traditional villages in South Henan region based on GIS analysis

open access: yesJournal of Asian Architecture and Building Engineering
Traditional villages serve as significant carriers of Chinese cultural heritage, embodying a rich history and unique regional characteristics, and hold considerable research value.
Xiaofang Bi   +3 more
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

open access: yesFEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour   +2 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

Research by design: interdisciplinary explorations within architectural design, structural design and material lab to develop innovative bio-based material pavilions

open access: yes, 2019
The Netherlands are at the forefront of an ambitious agenda: to make the building industry fully circular by 2050. In this context this interdisciplinary ‘research by design’ master project explored, tested and experimented with bio-based composite ...
Schröder, Torsten   +6 more
core  

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

open access: yesFEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová   +2 more
wiley   +1 more source

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