Results 101 to 110 of about 720,601 (301)

The use of building simulation within an architectural practice [PDF]

, 2003
This paper documents the development and implementation and use of simulation within an architectural practice and reports how its use facilitates the practice's commitment to Sustainable ...
Hobbs, Dan, Morbitzer, Christoph, Spires, Brian, Strachan, Paul, Webster, Jim   +4 more
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Potential Assessment and Community Environment Support Strategies for Social Interaction, Based on the Spatiotemporal Behavior of Accompanying Elderly Migrants: A Case Study in Hangzhou

Land
With the growing trend of population mobility and the aging process in China, a significant number of accompanying elderly migrants have moved to major cities.
Zhi Qiu, Bo Jin, Binwei Yun, Zhu Wang, Xincheng Pu   +4 more
doaj   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Spatiotemporal changes in human footprints and vegetation coverage and their impacts on the ecosystem services value in the Yangtze River Basin, China

Journal of Freshwater Ecology
In the face of river basin environmental challenges and the urgent need for sustainable development strategies, understanding the complex interactions among ecological services, human activities, and vegetation cover is of paramount importance.
Zhenyu Zhang, Haidong Li, Zhengyu Zhao, Wenjun Zhao, Yuxin Zhang, Yunxing Zhang, Zhigang Li   +6 more
doaj   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Architectural Design Quality in Local Authority Private Finance Initiative Projects [PDF]

, 2010
Since the 1990s, when the Private Finance Initiative was developed as the primary method for delivering major public capital projects, there has been concern about the quality of many of the products.
Giddings, Bob, Jensen, Phil, Jones, Paul, Sharma, Monika   +3 more
core  

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Management into design education: a case study [PDF]

, 2005
The design project set in a studio learning environment remains central to much of the under- graduate curriculum activity for the aspiring architect. Yet much recent discussion has identified the need to look beyond this design curriculum horizon and to
Beacock, Peter, Daws, Michael
core  

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source