Results 81 to 90 of about 369,728 (357)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Developing digital literacy in construction management education: a design thinking led approach [PDF]

, 2013
Alongside the digital innovations in AEC (Architectural, Engineering and Construction) practice, are calls for a new type of digital literacy, including a new information-based literacy informed by creativity, critical analysis and the theoretical and ...
Awad, Ramsey, Jupp, Julie
core   +1 more source

The role of virtual reality in built environment education [PDF]

, 2008
This study builds upon previous research on the integration of Virtual Reality (VR) within the built environment curriculum and aims to investigate the role of VR and three-dimensional (3D) computer modelling on learning and teaching in a school of the ...
Alvarado R. G., Ausburn L. J., Denscombe M., Horne M., Knight P., Mantovani F., Stone R.   +6 more
core   +2 more sources

Real‐World Investigation of Satralizumab in Patients With Neuromyelitis Optica Spectrum Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Satralizumab, a monoclonal antibody targeting the interleukin‐6 receptor, has demonstrated efficacy in clinical trials for neuromyelitis optica spectrum disorder (NMOSD). However, its real‐world effectiveness and safety compared to conventional immunosuppressive therapies remain uncertain.
Li‐Tsung Lin, Hui‐An Lin, Sheng‐Feng Lin   +2 more
wiley   +1 more source

Typomorphology: A Methodological Approach to Context Analysis in Architectural Design Education

Iconarp International Journal of Architecture and Planning
The study aims to demonstrate the importance of typomorphological knowledge as a method of contextual analysis in architectural design education. The Architectural Design Studio is a place for students to experience design tools, methods, and practice ...
Seda Saylan, Tan Kâmil Gürer
doaj   +1 more source

an Introduction to Architectural Education [PDF]

صفه, 1991
The architectural body of our country, Iran , is at the moment passing through a period known as" The Crisis of Identity" Therefore it is the task of all experts to use their energy in the swift passage of this period to reach an era of cultural and self
Hadi Nadimi
doaj  

Management into design education: a case study [PDF]

, 2005
The design project set in a studio learning environment remains central to much of the under- graduate curriculum activity for the aspiring architect. Yet much recent discussion has identified the need to look beyond this design curriculum horizon and to
Beacock, Peter, Daws, Michael
core  

Deep Learning–Assisted Differentiation of Four Peripheral Neuropathies Using Corneal Confocal Microscopy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Peripheral neuropathies contribute to patient disability but may be diagnosed late or missed altogether due to late referral, limitation of current diagnostic methods and lack of specialized testing facilities. To address this clinical gap, we developed NeuropathAI, an interpretable deep learning–based multiclass classification ...
Chaima Ben Rabah, Ioannis N. Petropoulos, Mark Stettner, Maryam Ferdousi, Uazman Alam, Nathan Efron, Ahmed Serag, Rayaz A. Malik   +7 more
wiley   +1 more source

Rules of Engagement: Architecture Theory and the Social Sciences in Frank Duffy’s 1974 Thesis on Office Planning [PDF]

This paper addresses the broad shift that took place in architectural theory and education in the 70s, where models of the discipline asserting the autonomy of architecture eclipsed models privileging architecture’s ties to other disciplines ...
Branden Hookway
core