Results 121 to 130 of about 90,450 (311)
Spatial biology in cancer epigenetics
Molecular Oncology, EarlyView.Spatial epigenomics combines molecular profiling with tissue architecture to reveal how gene regulation is organized within intact tissues. In cancer, these technologies uncover the mechanisms driving tumor heterogeneity and microenvironmental interactions, opening new opportunities for biomarker discovery and precision medicine.
Eva Crespo‐García, Manel Esteller
wiley +1 more source
Architectural Extraction In Reverse Engineering by Prototyping: An experiment [PDF]
, 1997 In this workshop proposal we present a prototype approach to help the extraction of architectural information in the re-engineering process. Commonly, the re-engineering life-cycle has been defined as a succession of the following tasks: analysis of ...
Tichelaar, Sander +2 more
core
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Evaluating Software Architectures: Development Stability and Evolution [PDF]
, 2003 We survey seminal work on software architecture evaluation methods. We then look at an emerging class of methods that explicates evaluating software architectures for stability and evolution. We define architectural stability and formulate the problem of
Rami Bahsoon +3 more
core
Архитектура, строительство, транспортThis article addresses the issue of integrating artificial intelligence into curricula and programs in the "Architecture" specialty. Al is considered as an essence of modern creative work and is analyzed as an integrative model in terms of its positive ...
O. Yu. Kostko +2 more
doaj +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
Swayne Field, architectural drawings, 1909 [PDF]
These architectural drawings were created for the Toledo Exhibition Company by the Osborn Engineering Company of Cleveland, Ohio. The drawings are white line on blue paper and include depictions of the grandstand, bleachers, an office, future bleachers ...
Osborn Engineering Company, Engineering Firm (Cleveland, Ohio)
core
ARTHUR: A Collaborative Augmented Environment for Architectural Design and Urban Planning [PDF]
, 2004 Projects in the area of architectural design and urban planning typically engage several architects as well as experts from other professions. While the design and review meetings thus often involve a large number of cooperating participants, the actual ...
Wittkämper, M +17 more
core
YIPFα1A expression is regulated by multilayered molecular mechanisms
FEBS Open Bio, EarlyView.YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji +2 more
wiley +1 more source