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Modularize and Unite: Toward Creating a Functional Artificial Cell [PDF]

Frontiers in Molecular Biosciences, 2021
An artificial cell is a simplified model of a living system, bringing breakthroughs into both basic life science and applied research. The bottom-up strategy instructs the construction of an artificial cell from nonliving materials, which could be ...
Chen Wang, Junzhu Yang, Yuan Lu
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Environment-Sensitive Intelligent Self-Reproducing Artificial Cell with a Modification-Active Lipo-Deoxyribozyme [PDF]

Micromachines, 2020
As a supramolecular micromachine with information flow, a giant vesicle (GV)-based artificial cell that exhibits a linked proliferation between GV reproduction and internal DNA amplification has been explored in this study.
Muneyuki Matsuo, Yuiko Hirata, Kensuke Kurihara, Taro Toyota, Toru Miura, Kentaro Suzuki, Tadashi Sugawara   +6 more
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Programmable Enzymatic Reaction Network in Artificial Cell‐Like Polymersomes [PDF]

Advanced Science
The ability to precisely control in vitro enzymatic reactions in synthetic cells plays a crucial role in the bottom‐up design of artificial cell models that can recapitulate the key cellular features and functions such as metabolism. However, integration
Hanjin Seo, Hyomin Lee
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Fabrication of a T-Shaped Microfluidic Channel Using a Consumer Laser Cutter and Application to Monodisperse Microdroplet Formation

Micromachines, 2021
The use of micrometer-sized droplets for chemical and biochemical analysis has been widely explored. Photolithography is mainly used to fabricate microfluidic devices, which is often employed to form monodisperse microdroplets.
Naoki Sasaki, Eisuke Sugenami
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Preliminary feasibility study using a solution of synthetic enzymes to replace the natural enzymes in polyhemoglobin-catalase-superoxide dismutase-carbonic anhydrase: effect on warm ischemic hepatocyte cell culture

Frontiers in Bioengineering and Biotechnology, 2023
This is a study on a simple solution of chemically prepared small chemical molecules of synthetic enzymes: catalase, superoxide dismutase, and carbonic anhydrase (CAT, SOD, and CA). We carried out a study to see if these synthetic enzymes can replace the
M. Hoq, T. M. S. Chang
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A Practical Guide to Preparation and Applications of Giant Unilamellar Vesicles Formed via Centrifugation of Water-in-Oil Emulsion Droplets

Membranes, 2023
Giant vesicles (GVs), which are closed lipid bilayer membranes with a diameter of more than 1 μm, have attracted attention not only as model cell membranes but also for the construction of artificial cells.
Yiting Zhang, Haruto Obuchi, Taro Toyota
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3D cell culture based on artificial cells and hydrogel under microgravity for bottom-up microtissue constructs

Frontiers in Bioengineering and Biotechnology, 2022
The use of hydrogel as a filling medium to recombine dispersed microencapsulated cells to form an embedded gel-cell microcapsule complex is a new idea based on bottom-up tissue construction, which is benefit for cell distribution and of great ...
Ruimin Long, Ruimin Long, Ruimin Long, Linrong Shi, Peng He, Jumei Tian, Shibin Wang, Shibin Wang, Shibin Wang, Jun Zheng   +9 more
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Theoretical perspective on synthetic man‐made life: Learning from the origin of life

Quantitative Biology, 2023
Creating a man‐made life in the laboratory is one of life science’s most intriguing yet challenging problems. Advances in synthetic biology and related theories, particularly those related to the origin of life, have laid the groundwork for further ...
Lu Peng, Zecheng Zhang, Xianyi Wang, Weiyi Qiu, Liqian Zhou, Hui Xiao, Chunxiuzi Liu, Shaohua Tang, Zhiwei Qin, Jiakun Jiang, Zengru Di, Yu Liu   +11 more
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Light energy transduction in liposome-based artificial cells

Frontiers in Bioengineering and Biotechnology, 2023
In this work we review the latest strategies for the bottom-up assembly of energetically autonomous artificial cells capable of transducing light energy into chemical energy and support internalized metabolic pathways.
Paola Albanese, Paola Albanese, Fabio Mavelli, Emiliano Altamura   +3 more
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Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology

Frontiers in Bioengineering and Biotechnology, 2020
Cell-free systems offer a promising approach to engineer biology since their open nature allows for well-controlled and characterized reaction conditions. In this review, we discuss the history and recent developments in engineering recombinant and crude
Nadanai Laohakunakorn, Laura Grasemann, Barbora Lavickova, Grégoire Michielin, Amir Shahein, Zoe Swank, Sebastian J. Maerkl   +6 more
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