Results 111 to 120 of about 7,311,828 (299)

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

An artificial development model for cell pattern generation

, 2007
Cell pattern formation has a crucial role in both artificial and natural development. This paper presents an artificial development model for cell pattern generation based on the cellular automata (CA) paradigm.
Duthen, Y., Chavoya, A.
core   +1 more source

MiR-122-5p inhibits the epithelial mesenchymal transition of liver cancer cells by inducing hiPSCs to differentiate into hepatocyte-like cells

European Journal of Histochemistry
Epithelial-mesenchymal transition (EMT) is closely linked to liver cancer prognosis, invasiveness, and aggressiveness. One promising treatment for liver cancer is cell therapy, where stem cells are stimulated to develop into functional liver cells. This
Qianzhe Xing, Yanjie Xu, Ying Luo, Chenglong Li, Peng Wang, Bin Kang, Chengjun Lu   +6 more
doaj   +1 more source

Stress-Induced Changes in Bone Marrow Stromal Cell Populations Revealed through Single-Cell Protein Expression Mapping

, 2019
International audienceStromal cell populations that maintain hematopoietic stem and progenitor cells (HSPCs) are generally characterized in steady-state conditions.
Scadden, David, Scadden, Elizabeth, Rhee, Catherine, Kfoury, Youmna, Karabacak, Nezihi Murat, Brouse, Thomas, Baryawno, Ninib, Courties, Gabriel, Nahrendorf, Matthias, Lee, Dongjun, Severe, Nicolas, Gustafsson, Karin, Toner, Mehmet, Kokkaliaris, Konstantinos, Garcia-Robledo, Juan Esteban, Kfoury, Y, Courties, G, Lee, D, Gustafsson, K, Baryawno, N,, Scadden, EW, Rhee, C, Severe, N, Karabacak, NM, Kokkaliaris, KD   +24 more
core   +1 more source

From patient advocacy to patient‐driven research: Building active partnerships beginning at the bench to reach the bedside

FEBS Open Bio, EarlyView.
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia, Rachel Heilmann, Maximiliano Barrientos, Laura MacNeill, Ingrid Vallee, Sarah K. Schultz, Patrick O'Donoghue, Angelica A. Moresco, Victoria M. Siu, Ilka U. Heinemann   +9 more
wiley   +1 more source

Stem Cell States, Fates, and the Rules of Attraction

, 2009
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different ...
Andrews, Peter W., Peterson, Carsten, Enver, Tariq, Pera, Martin, Peterson, Carsten,   +4 more
core   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Investigation of Genipin Cross-Linked Microcapsule for Oral Delivery of Live Bacterial Cells and Other Biotherapeutics: Preparation and In Vitro Analysis in Simulated Human Gastrointestinal Model

International Journal of Polymer Science, 2010
Oral therapy utilizing engineered microorganisms has shown promise in the treatment of many diseases. By microencapsulation, viable cells can overcome the harsh gastrointestinal (GI) environment and secrete needed therapeutics into the gut.
Hongmei Chen, Wei Ouyang, Christopher Martoni, Fatemeh Afkhami, Bisi Lawuyi, Trisna Lim, Satya Prakash   +6 more
doaj   +1 more source

Sensors of Succinate : Neural Stem Cell Grafts Fight Neuroinflammation

, 2018
In this issue of Cell Stem Cell, Peruzzotti-Jametti et al. (2018) demonstrate how neural stem cells, transplanted in a mouse model of multiple sclerosis, respond to extracellular succinate and modulate neuroinflammation by releasing anti-inflammatory ...
Olle Lindvall, Kokaia, Zaal, Zaal Kokaia, Lindvall, Olle, Kokaia, Zaal,, Lindvall, Olle,, Lund University.   +6 more
core   +1 more source