Results 221 to 230 of about 13,526 (245)
Some of the next articles are maybe not open access.
Ascorbate peroxidase activity of cytochromec
Free Radical Research, 2010The peroxidase-type reactivity of cytochrome c is proposed to play a role in free radical production and/or apoptosis. This study describes cytochrome c catalysis of peroxide consumption by ascorbate. Under conditions where the sixth coordination position at the cytochrome c heme iron becomes more accessible for exogenous ligands (by carboxymethylation,
Bischin, Cristina +6 more
openaire +3 more sources
Cross reactivity between ascorbate peroxidase and phenol (guaiacol) peroxidase
Postharvest Biology and Technology, 2014Abstract Adverse conditions often induce an increase in active oxygen species (AOS) such as hydrogen peroxide (H 2 O 2 ). H 2 O 2 is converted to water, and thus becomes detoxified by enzymes such as ascorbate peroxidase (APX; EC 1.11.1.11). APX activity is estimated by the disappearance rate of ascorbic acid, which becomes oxidized.
Wouter G. van Doorn, Saichol Ketsa
openaire +1 more source
Soluble ascorbate peroxidase from potato tubers
Plant Science, 1992Abstract Ascorbate peroxidase (EC 1.11.1.11) from potato tubers was purified to a nearly homogeneous state. The enzyme occurred only in the soluble fraction and showed to be labile in the absence of ascorbate. It had a r of ∼30 kDa, a pH optimum of ∼7, a K m for hydrogen peroxide of 30 μM and a K m for ascorbate of 55 μM.
Maria Rosaria Elia +2 more
openaire +1 more source
Crystal structure of recombinant pea cytosolic ascorbate peroxidase
Biochemistry, 1995The crystal structure of recombinant pea cytosolic ascorbate peroxidase has been refined to an R = 0.19 for data between 8.0 and 2.2 A resolution and magnitude of F > or = 2 sigma(magnitude of F). The refined model consists of four ascorbate peroxidase monomers consisting of 249 residues per monomer assembled into two homodimers, with one heme group ...
W R, Patterson, T L, Poulos
openaire +2 more sources
2000
Ascorbate-dependent peroxidase activity was first reported in 1979 (Groden and Beck, 1979; Kelly and Latzko, 1979) and ascorbate peroxidase (APX) is, therefore, a relative newcomer to the peroxidase field--horseradish (HRP) and cytochrome c (CcP) peroxidases were, for example, first identified in 1903 (Bach and Chodat, 1903) and 1940 (Altschul et al ...
openaire +2 more sources
Ascorbate-dependent peroxidase activity was first reported in 1979 (Groden and Beck, 1979; Kelly and Latzko, 1979) and ascorbate peroxidase (APX) is, therefore, a relative newcomer to the peroxidase field--horseradish (HRP) and cytochrome c (CcP) peroxidases were, for example, first identified in 1903 (Bach and Chodat, 1903) and 1940 (Altschul et al ...
openaire +2 more sources
Catalytic Oxidation of p-Cresol by Ascorbate Peroxidase
Archives of Biochemistry and Biophysics, 2000Transient and steady state kinetics, together with a range of chromatographic and spectroscopic techniques, have been used to establish the mechanism and the products of the H(2)O(2)-dependent oxidation of p-cresol by ascorbate peroxidase (APX). HPLC, GC-MS, and NMR analyses are consistent with the formation of 2, 2'-dihydroxy-5,5'-dimethylbiphenyl (II)
Celik, A, Cullis, PM, Lloyd Raven E
openaire +3 more sources
New Phytologist, 2011
• Peroxidases are involved in several important processes, such as development and responses to environmental cues. In higher plants, most peroxidases are encoded by large, multigenic families that mainly originated from gene and chromosomal duplications.
Fernanda, Lazzarotto +9 more
openaire +2 more sources
• Peroxidases are involved in several important processes, such as development and responses to environmental cues. In higher plants, most peroxidases are encoded by large, multigenic families that mainly originated from gene and chromosomal duplications.
Fernanda, Lazzarotto +9 more
openaire +2 more sources
Effectiveness of ascorbate and ascorbate peroxidase in promoting nitrogen fixation in model systems
Phytochemistry, 1999Ascorbate and ascorbate peroxidase are important antioxidants that are abundant in N2-fixing legume root nodules. Antioxidants are especially critical in root nodules because leghemoglobin, which is present at high concentrations in nodules, is prone to autoxidation and production of activated oxygen species such as O2.- and H2O2.
E J, Ross, S B, Kramer, D A, Dalton
openaire +2 more sources
Distribution of cytosolic ascorbate peroxidase in Angiosperms
Giornale botanico italiano, 1996Abstract Over 80 Angiosperms have been screened for cytosolic ascorbate peroxidase by means of native-polyacrylamide gel electrophoresis techniques. The results of our analysis show that the presence of a single cytosolic ascorbate peroxidase form is the most common case in the Angiosperms investigated when seedlings or young tissues are analized. This
PACIOLLA, Costantino +3 more
openaire +1 more source
Physiological function and regulation of ascorbate peroxidase isoforms
Journal of Experimental BotanyAbstractAscorbate peroxidase (APX) reduces H2O2 to H2O by utilizing ascorbate as a specific electron donor and constitutes the ascorbate–glutathione cycle in organelles of plants including chloroplasts, cytosol, mitochondria, and peroxisomes. It has been almost 40 years since APX was discovered as an important plant-specific H2O2-scavenging enzyme ...
Kazuya Yoshimura, Takahiro Ishikawa
openaire +2 more sources