Results 171 to 180 of about 202,771 (390)

Development and Validation of a High‐Throughput Screening Assay for the Legionella ADP‐Ribosyl Transferase SdeA

open access: yesChemBioChem, EarlyView.
We show the optimization and application of a fluorogenic assay to monitor Legionella SdeA activity in a High‐Throughput‐Screening campaign. Ubiquitination of proteins is one of the most crucial post‐translational modifications in eukaryotic cells, typically involving conjugation of ubiquitin to a lysine residue in a substrate using a three‐enzyme ...
Halana C. Vlaming   +6 more
wiley   +1 more source

Improved synchronous production of Plasmodium falciparum gametocytes in vitro.

open access: yes, 2007
The sexual stages of the Plasmodium falciparum life cycle are attractive targets for vaccines and transmission blocking drugs. Difficulties in culturing and obtaining large amounts of sexual stage P.
Baker, David A   +7 more
core   +1 more source

Deserts, Rivers, Pools, and Billabongs: Water Features of the Nitrogenase Proteins, and their Functions

open access: yesChemBioChem, EarlyView.
In the protein surrounding the active site of nitrogenase there is a large anhydrous desert, traversed by two watercourses necessary for the enzyme to convert N2 to NH3. Various pools exist, but their water seems to be stagnant. This review examines the occurrence and function of water inside the protein that contains the catalytic site of the enzyme ...
Ian Dance
wiley   +1 more source

Cellular and extracellular siderophores of Aspergillus nidulans and Penicillium chrysogenum [PDF]

open access: yes, 1981
Aspergillus nidulans and Penicillium chrysogenum produce specific cellular siderophores in addition to the well-known siderophores of the culture medium.
Charlang, Gisela   +3 more
core  

Chemical Methods for Peptide and Protein Backbone Cleavage

open access: yesChemistryEurope, EarlyView.
Protein cleavage plays essential roles in biology and is powerful for protein manipulation and functionalization as well as in recombinant expression and purification. Here, the historical background and most recent examples of chemical methods for protein and peptide backbone cleavage are discussed (part of the figure was generated in BioRender).
Miguel Angel Alena‐Rodriguez   +1 more
wiley   +1 more source

Optimization of rTDMH as a Reagent Toward Improving the Sensitivity of the RT-PCR Based Diagnosis for Mycobacterium Tuberculosis [PDF]

open access: yes, 2012
Current diagnostic tools being used for tuberculosis lack the speed and sensitivity necessary to successfully combat the current tuberculosis epidemic.
Bhatti, Alexandra
core  

Pd(II)‐Catalyzed Strategies for the β‐Arylation of α‐Amino Acids: An Overview

open access: yesChemistry – A European Journal, EarlyView.
The graphical abstract summarizes the review “Pd(II)‐Catalyzed Strategies for the β‐Arylation of α‐Amino Acids: An Overview”, highlighting representative Pd(II)‐catalyzed methods for the β‐arylation of α‐amino acids. Key mechanistic features, substrate diversity, and synthetic relevance of these transformations are showcased. Abstract Metal‐catalyzed C─
Davide Illuminati   +4 more
wiley   +1 more source

Green Fluorescent Protein (GFP) in Vector Systems Played Sense Role of Epigenetic in Plants [PDF]

open access: yes, 2008
The green fluorescent protein (GFP) of jellyfish (_Aequorea victoria_) has significant advantages over other reporter genes, because expression can be detected in living cells without any substrates.
Hany A. El-Shemy   +2 more
core   +1 more source

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