Results 341 to 350 of about 414,727 (380)
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T-lymphoblastic leukaemia and aspartate aminotransferase
Leukemia Research, 1982AST levels from 11 untreated children with T-ALL were found to be significantly higher than those from 74 children with non-T disease. The enzyme was not related to haemoglobin or bilirubin levels nor to the presence of hepatosplenomegaly in any of the patients.
A.J. Kay +3 more
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Immobilization of aspartate aminotransferase on agarose
Biochimie, 1989Various methods for immobilization of aspartate aminotransferase (AspAT; from cytosolic fraction of pig heart) on agarose were tested. Aldehyde-, thiol-, and CNBr-activated agaroses were studied in detail. The capacity of the aldehyde support to firmly bind protein was less than 0.2 mg/ml, whereas the apparent remaining specific activity of the bound ...
Timo Korpela, Kalevi Kurkijärvi
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Aspartate aminotransferase isoenzymes
Clinical Biochemistry, 1990Aspartate aminotransferase (AST, EC 2.6.1.1) exists in human tissues as two distinct isoenzymes, one located in the cytoplasm (c-AST), and the other in mitochondria (m-AST). Striated muscle, myocardium, and liver tissues are the main sources of AST. A growing body of information suggests that determination of AST isoenzymes in human serum is useful in ...
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Aminotransferases in Grapes. Isolation and Characterization of Aspartate Aminotransferase
American Journal of Enology and Viticulture, 1991International ...
Sauvage, F.X. +3 more
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Stability of aspartate aminotransferase and alanine aminotransferase activities.
The Journal of laboratory and clinical medicine, 1983Because there are conflicting data regarding the effect of different temperatures and durations of storage on the stability of the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), a new study has been conducted to re-examine this important issue.
Hyman J. Zimmerman +4 more
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Aspartate aminotransferase in the brain of the developing rat
Life Sciences, 1965Abstract The biochemical immaturity of the rat brain at birth has been largely investigated in recent years (see for references, Sperry, 1955, 1962; Himwich, 1962). It has been tempting to relate neurochemical data to older findings on the physiological maturation of the organ.
Giuseppe Amore, Vincenzo Bonavita
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Determination of mitochondrial aspartate aminotransferase in serum
Clinica Chimica Acta, 1986Two specific and sensitive immunoassay methods for the determination of mitochondrial aspartate aminotransferase (m-AST) are described. One is a sandwich enzyme immunoassay which measures immunologically active m-AST using polystyrene balls coated with anti-m-AST antibody and peroxidase-labelled anti-m-AST antibody as the second antibody. The detection
Yasutoshi Muto +6 more
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The rotational relaxation time of aspartate aminotransferase
Biochimica et Biophysica Acta (BBA) - Protein Structure, 1967Abstract Two independent methods, sucrose density centrifugation and polarization of fluorescence, were used to study the molecular state of the enzyme aspartate amino-transferase at concentrations approaching those used in the enzymatic assays. 1. 1.
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The binding of bromophenol blue to aspartate aminotransferase
Archives of Biochemistry and Biophysics, 1976Abstract Several experiments are presented that suggest that the sulfonphthalein dye, bromophenol blue, binds at, or near to, the active site of pig heart extramitochondrial aspartate aminotransferase (EC 2.6.1.1.). The binding is characterized at pH 8.0 by a bathochromic shift in the dye's visible spectrum from 590 to 599 nm.
W.Terry Jenkins, Richard C. Harruff
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Redesign of aspartate aminotransferase specificity to that of tyrosine aminotransferase
1994The values of kcat/Km are strongly correlated with chain length for the reactions of E. coli tyrosine aminotransferase, but are nearly independent of this variable for aspartate aminotransferase. Both enzymes exhibit nearly equal reactivity with dicarboxylic acid substrates.
James J. Onuffer, Jack F. Kirsch
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