Results 101 to 110 of about 832,569 (293)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Frowning Frodo, Wincing Leia, and a Seriously Great Friendship: Learning to Classify Emotional Relationships of Fictional Characters

, 2019
The development of a fictional plot is centered around characters who closely interact with each other forming dynamic social networks. In literature analysis, such networks have mostly been analyzed without particular relation types or focusing on roles
Kim, Evgeny, Klinger, Roman
core   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

A vocabulary-independent generation framework for DBpedia and beyond [PDF]

, 2017
The dbpedia Extraction Framework, the generation framework behind one of the Linked Open Data cloud’s central hubs, has limitations which lead to quality issues with the dbpedia dataset.
De Meester, Ben, Dimou, Anastasia, Hellman, S., Kontokostas, D, Lehmann, J., Mannens, Erik, Maroy, Wouter, Verborgh, Ruben   +7 more
core  

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Aspect-Based Sentiment Analysis: A Comprehensive Review and Open Research Challenges

IEEE Access
The social web provides a facility for common people to share their views, comments, feedback, and experiences on various social media platforms. Due to these platforms now communication has become easier and it has provided us with opportunities to use ...
Waqas Ahmad, Hikmat Ullah Khan, Fawaz Khaled Alarfaj, Mohammed Alreshoodi   +3 more
doaj   +1 more source

Bidirectional Cascade Network for Aspect Sentiment Triplet Extraction [PDF]

Jisuanji gongcheng
Aspect Sentiment Triplet Extraction(ASTE) is a subtask of aspect-based sentiment analysis. The goal is to extract all mentioned aspects, their corresponding opinions, and the sentiment polarity expressed, forming triplets from a given text sequence.
ZHENG Ayong, GU Xingsheng
doaj   +1 more source

A supervised scheme for aspect extraction in sentiment analysis using the hybrid feature set of word dependency relations and lemmas. [PDF]

PeerJ Comput Sci, 2021
Bhamare BR, Prabhu J.
europepmc   +1 more source

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

The cooperative regulation of miR‐221 by APE1 and AUF1 impacts p27Kip1 defining a miR signature relevant for cervical cancer

FEBS Open Bio, EarlyView.
A regulatory axis involving APE1, AUF1, and miR‐221 is proposed. Pri‐miR‐221 is processed by DROSHA and DICER to generate mature miR‐221, which targets p27Kip1 mRNA. APE1 and AUF1 compete for pre‐miR‐221 binding. Reduced APE1/AUF1 levels impair miR‐221 biogenesis, decrease p27Kip1 mRNA degradation, and promote cell cycle progression, chemoresistance ...
Matilde Clarissa Malfatti, Nicolò Gualandi, Gianluca Tell, Giulia Antoniali   +3 more
wiley   +1 more source