Results 11 to 20 of about 4,614,031 (312)

EndoBind detects endogenous protein-protein interactions in real time

open access: yesCommunications Biology, 2021
Bill et al describe two high-throughput methods to detect protein-protein interactions in cells in real-time using the split-NanoLuciferase-complementation system.
Anke Bill   +12 more
doaj   +1 more source

Assaying mechanosensation [PDF]

open access: yesWormBook, 2014
C. elegans detect and respond to diverse mechanical stimuli using neuronal circuitry that has been defined by decades of work by C. elegans researchers. In this WormMethods chapter, we review and comment on the techniques currently used to assess mechanosensory response. This methods review is intended both as an introduction for those new to the field
Martin, Chalfie   +3 more
openaire   +2 more sources

Validation of Commercial SARS-CoV-2 Immunoassays in a Nigerian Population

open access: yesMicrobiology Spectrum, 2021
Validated assays are essential for reliable serosurveys; however, most SARS-CoV-2 immunoassays have been validated using specimens from China, Europe, or U.S. populations.
Fehintola Ige   +10 more
doaj   +1 more source

Comprehensive Comparison of Seven SARS-CoV-2-Specific Surrogate Virus Neutralization and Anti-Spike IgG Antibody Assays Using a Live-Virus Neutralization Assay as a Reference

open access: yesMicrobiology Spectrum, 2023
Neutralizing antibodies (nAbs) are considered a valuable marker for measuring humoral immunity against SARS-CoV-2. However, live-virus neutralization tests (NTs) require high-biosafety-level laboratories and are time-consuming. Therefore, surrogate virus
Marianne Graninger   +14 more
doaj   +1 more source

Potential pitfalls in diagnosis of immunotherapy-induced hypothalamic–pituitary–adrenal axis abnormalities: a clinical case

open access: yesEndocrine Oncology, 2023
Short synacthen tests (SST) are frequently used for assessing adrenocorticotropin hormone (ACTH) deficiency. In this study, we present the case of a 53-year-old man receiving immunotherapy for metastatic melanoma, who subsequently developed immune ...
Yixi Bi   +4 more
doaj   +1 more source

Effect of antimicrobial exposure on AcrAB expression in Salmonella enterica subspecies enterica serovar Choleraesuis

open access: yesFrontiers in Microbiology, 2013
Understanding the impact of antimicrobial use on the emergence of resistant bacteria is imperative to prevent its emergence. For instance, activation of the AcrAB efflux pumps is responsible for the emergence of antimicrobial-resistant Salmonella strains.
Masaru eUsui   +5 more
doaj   +1 more source

Topoisomerase Assays [PDF]

open access: yesCurrent Protocols in Pharmacology, 2012
AbstractTopoisomerases are nuclear enzymes that play essential roles in DNA replication, transcription, chromosome segregation, and recombination. All cells have two major forms of topoisomerases: type I enzymes, which make single‐stranded cuts in DNA, and type II enzymes, which cut and pass double‐stranded DNA. DNA topoisomerases are important targets
John L, Nitiss   +4 more
openaire   +2 more sources

Total nitrogen and l-hydroxyproline content of adipose tissue from various sites in rats of different ages

open access: yesJournal of Lipid Research, 1970
The total nitrogen and l-hydroxyproline concentrations of two intra-abdominal and two subcutaneous sites of adipose tissue were measured in male and female Wistar rats.At 100 g body weight the nitrogen concentration of intra-abdominal adipose tissue was ...
P.B. Curtis-Prior   +3 more
doaj   +1 more source

NEW APPLICATIONS FOR AMOXICILLIN DETERMINATION IN PURE FORM AND PHARMACEUTICALS BASED ON IODATE-IODIDE MIXTURE: TITRIMETRY AND SPECTROSCOPY STUDIES [PDF]

open access: yesQuímica Nova, 2020
Three simple methods are described for the determination of amoxicillin (AMX) in bulk drug and dosage forms using iodate-iodide mixture reagent. These methods employed the well-known analytical reaction between iodate-iodide mixture in the presence of ...
Nagib A. S. Qarah   +4 more
doaj   +1 more source

Immunogenicity of TNF-Inhibitors

open access: yesFrontiers in Immunology, 2020
Tumor necrosis factor inhibitors (TNFi) have significantly improved treatment outcome of rheumatic diseases since their incorporation into treatment protocols two decades ago. Nevertheless, a substantial fraction of patients experiences either primary or
Sadaf Atiqi   +5 more
doaj   +1 more source

Home - About - Disclaimer - Privacy