Journal of Chromatography A, 2020 Electrical asymmetrical flow field-flow fractionation (EAF4) is an interesting new analytical technique that separates proteins based on size or molecular weight and simultaneously determines the electrical characteristics of each population.
Jaeyeong Choi +4 more
semanticscholar +3 more sources
Asymmetrical flow field-flow fractionation and multi-angle laser light scattering: A new analytical approach for the characterisation of insect protein aggregation/polymerisation after heat treatment of Tenebrio molitor larvae [PDF]
Current Research in Food ScienceUnderstanding the structural modifications of insect proteins during the transformation processes used for extract preparation is essential for optimising their functionalities and obtaining high added-value proteins.
Ariel Anouma +3 more
doaj +2 more sources
Screening for Protein-Protein Interactions with Asymmetrical Flow Field-Flow Fractionation. [PDF]
Journal of Pharmaceutical Sciences, 2021 We describe a new method for screening protein-protein interaction of biopharmaceutical molecules at dilute concentrations to predict development issues at high concentration.
Per-Olof Wahlund +2 more
semanticscholar +3 more sources
Asymmetrical Flow Field-Flow Fractionation on Virus and Virus-Like Particle Applications. [PDF]
Microorganisms, 2019 Asymmetrical flow field-flow fractionation (AF4) separates sample components based on their sizes in the absence of a stationary phase. It is well suited for high molecular weight samples such as virus-sized particles. The AF4 experiment can potentially separate molecules within a broad size range (~103−109 Da; particle diameter from 2 nm to 0.5−1 μm).
Eskelin K, Poranen MM, Oksanen HM.
europepmc +5 more sources
Key Methodologies in Characterizing the Multi-Scale Structures of Gluten Proteins in Dough: A Comparative Review [PDF]
BiomoleculesGluten proteins are key components in wheat flour that determine the formation of dough and the quality of flour-based products. Upon hydration and mixing, gluten proteins undergo complex structural transformations to form a gluten network, exhibiting a ...
Feifei Su +6 more
doaj +2 more sources
A One‐Step Workflow for Size‐Based Separation of Extracellular Vesicles With Integrated Surface Marker Detection [PDF]
Journal of Extracellular BiologyExtracellular vesicles (EVs) are released by diverse cell types in biofluids and are increasingly studied in liquid biopsies as diagnostic and prognostic biomarkers for various diseases, including cancer.
Lien Lippens +13 more
doaj +2 more sources
Physicochemical characterization and study of molar mass of industrial gelatins by AsFlFFF-UV/MALS and chemometric approach. [PDF]
PLoS ONE, 2018 Industrial gelatins have different physicochemical properties that mainly depend of the raw materials origin and the extraction conditions. These properties are closely related to the molar mass distribution of these gelatins.
Simon Duthen +6 more
doaj +1 more source
Asymmetric flow field‐flow fractionation of liposomes: optimization of fractionation variables [PDF]
Journal of Separation Science, 2009 AbstractThe purpose of this study was to investigate the influence of ionic strength of the carrier liquid, cross flow rate, focus flow rate, and sample load on the retention behavior of liposomes in asymmetric flow field‐flow fractionation (AF4). Two differently prepared samples of large unilamellar vesicles (LUV) were used. Experiments were performed
Stefan, Hupfeld +2 more
openaire +2 more sources
European journal of pharmaceutics and biopharmaceutics, 2021 Lipid-based nanoparticles for RNA delivery (LNP-RNA) are revolutionizing the nanomedicine field, with one approved gene therapy formulation and two approved vaccines against COVID-19 as well as multiple ongoing clinical trials.
R. Mildner +7 more
semanticscholar +1 more source
Current Research in Food Science, 2021 The dynamics of β-casein and casein micelles in the reconstitution of skim milk were revisited in this study. β-casein migrates into casein micelles upon an increase in temperatures due to an increase in the hydrophobic effect and lower calcium-phosphate
Anouk Lie-Piang +4 more
doaj +1 more source