Understanding the Degradation of Core-Shell Nanogels Using Asymmetrical Flow Field Flow Fractionation [PDF]
Journal of Functional Biomaterials, 2023 Nanogels are candidates for biomedical applications, and core-shell nanogels offer the potential to tune thermoresponsive behaviour with the capacity for extensive degradation.
Edyta Niezabitowska +5 more
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Analysis of Silicon Quantum Dots and Serum Proteins Interactions Using Asymmetrical Flow Field-Flow Fractionation. [PDF]
Langmuir, 2023 Semiconductor nanocrystals or quantum dots (QDs) have gained significant attention in biomedical research as versatile probes for imaging, sensing, and therapies.
Nagarajan U +4 more
europepmc +2 more sources
Offline Coupling of Asymmetrical Flow Field-Flow Fractionation and Capillary Electrophoresis for Separation of Extracellular Vesicles. [PDF]
Anal Chem, 2022 Extracellular vesicles (EVs) play important roles in cell-to-cell communications and carry high potential as markers targeted in disease diagnosis, prognosis, and therapeutic development.
Gao Z, Hutchins Z, Li Z, Zhong W.
europepmc +2 more sources
Enhancing Extracellular Vesicle Analysis by Integration of Large-Volume Sample Stacking in Capillary Electrophoresis with Asymmetrical Flow Field-Flow Fractionation. [PDF]
Anal Chem, 2023 Extracellular vesicles (EVs) play important roles in cell-cell communication and pathological development. Cargo profiling for the EVs present in clinical specimens can provide valuable insights into their functions and help discover effective EV-based ...
Gao Z +4 more
europepmc +2 more sources
Characterizing Non-covalent Protein Complexes Using Asymmetrical Flow Field-Flow Fractionation On-Line Coupled to Native Mass Spectrometry. [PDF]
Anal Chem, 2023 We report an online analytical platform based on the coupling of asymmetrical flow field-flow fractionation (AF4) and native mass spectrometry (nMS) in parallel with UV-absorbance, multi-angle light scattering (MALS), and differential-refractive-index ...
Ventouri IK +9 more
europepmc +2 more sources
Proteomics protocol for obtaining extracellular vesicle from human plasma using asymmetrical flow field-flow fractionation technology [PDF]
STAR Protocols, 2023 Summary: Plasma extracellular vesicles (EVs) represent a potential resource for biomarkers of multiple diseases. Here, we present a protocol for obtaining EVs from human plasma using asymmetrical flow field-flow fractionation technology.
Na Li +4 more
doaj +2 more sources
Investigation of native and aggregated therapeutic proteins in human plasma with asymmetrical flow field-flow fractionation and mass spectrometry. [PDF]
Anal Bioanal Chem, 2022 Physiochemical degradation of therapeutic proteins in vivo during plasma circulation after administration can have a detrimental effect on their efficacy and safety profile. During drug product development, in vivo animal studies are necessary to explore
Ramm I +5 more
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CHIMIA, 2022 Asymmetrical flow field-flow fractionation (AF4) is a powerful technique employed for the separation of macromolecules, nanoparticles, and their assemblages according to their hydrodynamic behavior.
Isabelle A. M. Worms, Vera I. Slaveykova
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A comprehensive study on the relationship between structure and antioxidant activity of natural deep eutectic solvent extracted polysaccharides from Chinese yam peel using asymmetrical flow field-flow fractionation [PDF]
Food Chemistry: XIn this study, an ultrasound-assisted natural deep eutectic solvents (NADES) combined with enzymatic deproteinization was developed for extracting Chinese Yam peel polysaccharide (CYPP) from two varieties.
Yao Huang +6 more
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Application of Asymmetrical Flow Field-Flow Fractionation for Characterizing the Size and Drug Release Kinetics of Theranostic Lipid Nanovesicles. [PDF]
Int J Mol Sci, 2021 Liposome size and in vitro release of the active substance belong to critical quality attributes of liposomal carriers. Here, we apply asymmetric flow field-flow fractionation (AF4) to characterize theranostic liposomes prepared by thin lipid film ...
Skupin-Mrugalska P, Elvang PA, Brandl M.
europepmc +2 more sources