Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine [PDF]
Journal of Extracellular Vesicles, 2018 Extracellular vesicles (EVs) have a great potential in clinical applications. However, their isolation from different bodily fluids and their characterisation are currently not optimal or standardised.
Eline Oeyen +10 more
doaj +5 more sources
Quantitative size-resolved characterization of mRNA nanoparticles by in-line coupling of asymmetrical-flow field-flow fractionation with small angle X-ray scattering [PDF]
Scientific Reports, 2023 We present a generically applicable approach to determine an extensive set of size-dependent critical quality attributes inside nanoparticulate pharmaceutical products. By coupling asymmetrical-flow field-flow fractionation (AF4) measurements directly in-
Melissa A. Graewert +14 more
doaj +3 more sources
Native RNA Purification Method for Small RNA Molecules Based on Asymmetrical Flow Field-Flow Fractionation [PDF]
Pharmaceuticals, 2022 RNA molecules provide promising new possibilities for the prevention and treatment of viral infections and diseases. The rapid development of RNA biology and medicine requires advanced methods for the purification of RNA molecules, which allow fast and ...
Alesia A. Levanova +5 more
doaj +3 more sources
Assessment of the Effects of Structural Modification of Gastrodia elata Polysaccharide on Anti-Breast Cancer Activity Using Asymmetrical Flow Field-Flow Fractionation [PDF]
Molecules, 2023 Gastrodia elata (“Tian Ma” in Chinese) is used as a food and medical ingredient in traditional Chinese medicine. In this study, to enhance the anti-breast cancer activity of Gastrodia elata polysaccharide (GEP), GEPs were modified via sulfidation (SGEP ...
Xiaoying Liu +7 more
doaj +3 more sources
Study of the Stability of Citrate Capped AgNPs in Several Environmental Water Matrices by Asymmetrical Flow Field Flow Fractionation [PDF]
Nanomaterials, 2021 Asymmetrical flow field-flow fractionation (AF4) coupled to UV-Vis and dynamic light scattering (DLS) detectors in series, was tested for stability studies of dispersions of citrate-capped silver nanoparticles (AgNPs) in several water matrices.
Aaron Boughbina-Portolés +3 more
doaj +3 more sources
Characterization and Quantitation of Carbon Black Nanomaterials in Polymeric and Biological Aqueous Dispersants by Asymmetrical Flow Field Flow Fractionation [PDF]
ACS Omega, 2021 Characterization of carbon black (CB) nanomaterials is required in industrial and research areas. Hence, in this study, asymmetrical flow field flow fractionation coupled to UV–vis and DLS detectors in series (AF4-UV–vis-DLS) was studied to evaluate the ...
Lorenzo Sanjuan-Navarro +2 more
doaj +3 more sources
Asymmetrical Flow Field-Flow Fractionation Methods for Quantitative Determination and Size Characterization of Thiols and for Mercury Size Speciation Analysis in Organic Matter-Rich Natural Waters [PDF]
Frontiers in Chemistry, 2022 Asymmetrical flow field-flow fractionation (AF4) efficiently separates various macromolecules and nano-components of natural waters according to their hydrodynamic sizes.
Isabelle A. M. Worms +4 more
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Nanoparticle separation with a miniaturized asymmetrical flow field-flow fractionation cartridge [PDF]
Frontiers in Chemistry, 2015 Asymmetrical Flow Field-Flow Fractionation (AF4) is a separation technique applicable to particles over a wide size range. Despite the many advantages of AF4, its adoption in routine particle analysis is somewhat limited by the large footprint of ...
David eMüller +5 more
doaj +7 more sources
Fast and Purification-Free Characterization of Bio-Nanoparticles in Biological Media by Electrical Asymmetrical Flow Field-Flow Fractionation Hyphenated with Multi-Angle Light Scattering and Nanoparticle Tracking Analysis Detection [PDF]
Molecules, 2020 Accurate physico-chemical characterization of exosomes and liposomes in biological media is challenging due to the inherent complexity of the sample matrix.
Roland Drexel +5 more
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Recovery, overloading, and protein interactions in asymmetrical flow field-flow fractionation. [PDF]
Anal Bioanal Chem, 2019 In asymmetrical flow field-flow fractionation (AF4), similar to other separation techniques, mass recovery and overloading require special attention in order to obtain quantitative results.
Marioli M, Kok WT.
europepmc +7 more sources