Proteins and antibodies in serum, plasma, and whole blood-size characterization using asymmetrical flow field-flow fractionation (AF4). [PDF]
Anal Bioanal Chem, 2018 The analysis of aggregates of therapeutic proteins is crucial in order to ensure efficacy and patient safety. Typically, the analysis is performed in the finished formulation to ensure that aggregates are not present.
Leeman M +4 more
europepmc +2 more sources
Perturbations of Lipids and Oxidized Phospholipids in Lipoproteins of Patients with Postmenopausal Osteoporosis Evaluated by Asymmetrical Flow Field-Flow Fractionation and Nanoflow UHPLC–ESI–MS/MS [PDF]
Antioxidants, 2020 Osteoporosis, a degenerative bone disease characterized by reduced bone mass and high risk of fragility, is associated with the alteration of circulating lipids, especially oxidized phospholipids (Ox-PLs).
Kang Geun Lee +3 more
doaj +2 more sources
J Control Release, 2020 Liposomal formulations for the treatment of cancer and other diseases are the most common form of nanotechnology enabled pharmaceuticals (NEPs) submitted for market approval and in clinical application today.
Parot J +4 more
europepmc +2 more sources
Controlled Disassembly and Purification of Functional Viral Subassemblies Using Asymmetrical Flow Field-Flow Fractionation (AF4) [PDF]
Viruses, 2018 Viruses protect their genomes by enclosing them into protein capsids that sometimes contain lipid bilayers that either reside above or below the protein layer.
Katri Eskelin, Minna M. Poranen
doaj +2 more sources
A comprehensive analysis in one run - in-depth conformation studies of protein-polymer chimeras by asymmetrical flow field-flow fractionation. [PDF]
Chem Sci, 2021 Polymer-based protein engineering has enabled the synthesis of a variety of protein–polymer conjugates that are widely applicable in therapeutic, diagnostic and biotechnological industries.
Kaupbayeva B +5 more
europepmc +2 more sources
Characterization of Silver Nanoparticles under Environmentally Relevant Conditions Using Asymmetrical Flow Field-Flow Fractionation (AF4). [PDF]
PLoS ONE, 2015 The development of methods to monitor manufactured nanomaterials in the environment is one of the crucial areas for the assessment of their risk. More specifically, particle size analysis is a key element, because many properties of nanomaterial are size
Min-Hee Jang, Seungho Lee, Yu Sik Hwang
doaj +2 more sources
Asymmetrical Flow Field Flow Fractionation Coupled to Nanoparticle Tracking Analysis for Rapid Online Characterization of Nanomaterials. [PDF]
Anal Chem, 2020 Increasing applications of nanomaterials in consumer goods, industrial products, medical practices, etc., calls for the development of tools for rapid separation, quantification and sizing of nanoparticles to ensure their safe and sustainable employment.
Adkins GB, Sun E, Coreas R, Zhong W.
europepmc +2 more sources
Fractionation of Nanoparticle Matter in Red Wines Using Asymmetrical Flow Field-Flow Fractionation. [PDF]
J Agric Food Chem, 2020 The particle matter of wine is mainly composed of wine colloids and macromolecules. The present work develops a methodology using asymmetrical flow field-flow fractionation coupled with multi-angle light scattering, differential refractive index detector, and ultraviolet detector (AsFlFFF-MALS-dRI-UV) for the fractionation and determination of the ...
Osorio-Macías DE +8 more
europepmc +4 more sources
Characterization of binding between model protein GA-Z and human serum albumin using asymmetrical flow field-flow fractionation and small angle X-ray scattering. [PDF]
PLoS ONE, 2020 Protein-based drugs often require targeted drug delivery for optimal therapy. A successful strategy to increase the circulation time of the protein in the blood is to link the therapeutic protein with an albumin-binding domain.
Jaeyeong Choi +7 more
doaj +2 more sources
Journal of Chromatography AAmong the plethora of techniques that conforms the Field-Flow Fractionation (FFF) family, electrical field-flow fractionation (ElFFF) was designed to separate different analytes based on their size and electrophoretic mobility (µ).
Armando Sánchez-Cachero +5 more
semanticscholar +3 more sources