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Present situation and development trends of asymmetrical flow field-flow fractionation

Chinese Journal of Chromatography, 2017
Field-flow fractionation (FFF) is a kind of mature separation technologies in the field of bioanalysis, feasible of separating analytes with the differences of certain physical and chemical properties by the combination effects of two orthogonal force fields (flow field and external force field).
Qihui, Liang   +3 more
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Optimization of asymmetrical flow field-flow fractionation

LC GC North America, 2011
Asymmetrical flow field-flow fractionation can be used on a routine basis to separate high molecular weight compounds as an alternative to size-exclusion chromatography (SEC), but the principle of separation is very different and a different approach is required for optimization.
Qureshi, R.N., Kok, W.T.
openaire   +1 more source

Size Characterization and Quantification of Exosomes by Asymmetrical-Flow Field-Flow Fractionation

Analytical Chemistry, 2015
In the past few years extracellular vesicles called exosomes have gained huge interest of scientific community since they show a great potential for human diagnostic and therapeutic applications. However, an ongoing challenge is accurate size characterization and quantification of exosomes because of the lack of reliable characterization techniques. In
Simona, Sitar   +6 more
openaire   +2 more sources

The utility of asymmetric flow field-flow fractionation for preclinical characterization of nanomedicines

Analytical and Bioanalytical Chemistry, 2019
Dynamic light scattering (DLS), transmission electron microscopy (TEM), and reversed phase-high performance liquid chromatography (RP-HPLC) are staples of nanoparticle characterization for size distribution, shape/morphology, and composition, respectively. These techniques are simple and provide important details on sample characteristics. However, DLS
Yingwen, Hu   +2 more
openaire   +2 more sources

Retention ratio and nonequilibrium bandspreading in asymmetrical flow field-flow fractionation

Analytical and Bioanalytical Chemistry, 2015
In asymmetrical flow field-flow fractionation (As-FlFFF), only the membrane-covered accumulation wall is permeable to fluid; the opposite channel wall is impermeable. Fluid enters the channel at the inlet and exits partly through the membrane-covered accumulation wall and partly through the channel outlet.
openaire   +2 more sources

Flow optimisations with increased channel thickness in asymmetrical flow field-flow fractionation

Journal of Chromatography A, 2018
Retention in flow field-flow fractionation (flow FFF) is generally governed by the combination of crossflow and migration flowrates. Especially for an asymmetrical flow FFF (AF4) channel in which the channel-inlet flow is divided into crossflow and outflow, the separation of low-molecular-weight proteins or macromolecules requires a relatively high ...
Joon Seon Yang, Myeong Hee Moon
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Characterization of Polymer Nanoparticles by Asymmetrical Flow Field Flow Fractionation (AF-FFF)

Journal of Nanoscience and Nanotechnology, 2010
We present the characterization of different polymeric nanoparticles with asymmetrical flow field-flow fractionation (AF-FFF) in different solvents and additional, independent methods such as static and dynamic light scattering (SLS, DLS) in solution and transmission electron microscopy (TEM) and atomic force microscopy (AFM) for the visualization of ...
Christian, Scherer   +6 more
openaire   +2 more sources

Asymmetric Field-Flow Fractionation in Biotechnology

2005
Thorsten Klein, Christine Hürzeler
openaire   +1 more source

Characterization of hydrocolloids by asymmetric flow field-flow fractionation

Proceedings of the 10th international conference on photoacoustic and photothermal phenomena, 1999
A. Exner   +4 more
openaire   +1 more source

Separation of Macromolecules by Miniaturized Asymmetrical Flow Field Flow Fractionation

2013
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Kralova, J.   +4 more
openaire   +1 more source

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