The use of asymmetrical flow field-flow fractionation in pharmaceutics and biopharmaceutics
European Journal of Pharmaceutics and Biopharmaceutics, 2004Field-flow fractionation (FFF) is a family of flexible analytical fractionating techniques which have the advantage that the separation of analytes is achieved, solely through the interaction of the sample with an external, perpendicular physical field, rather than by the interaction with a stationary phase.
Wolfgang, Fraunhofer, Gerhard, Winter
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Fractionation of Prion Protein Aggregates by Asymmetrical Flow Field‐Flow Fractionation
2006Achieving the successful separation and analysis of amyloid and other large protein aggregates can be a difficult proposition. Field-flow fractionation (FFF) is a flow-based separation method like chromatography; however, FFF is capable of high-resolution separations in the absence of a stationary matrix.
Jay R, Silveira +2 more
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Asymmetrical flow field-flow fractionation for the analysis of PEG-asparaginase
Talanta, 2016Monomethoxypolyethylene glycol L-asparaginase (PEG-ASNASE) is the PEGylated version of the enzyme L-asparaginase (ASNASE). Both are used for remission induction in acute lymphoblastic leukemia (ALL) and non-Hodgkin's lymphoma (NHL). The treatment control is generally carried out by performing activity assays, though methods to determine the actual ...
C, John +4 more
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Miniaturization of Frit Inlet Asymmetrical Flow Field-Flow Fractionation
Analytical Chemistry, 2004A miniaturized frit inlet asymmetrical flow field-flow fractionation (mFI-AFlFFF) channel has been constructed and tested for the separation of proteins. By scaling down the geometrical channel dimension of a conventional FI-AFlFFF system, flow rate ranges that can be manipulated were decreased to 20-30 microL/min, which reduces the injection amount of
Dukjin, Kang, Myeong Hee, Moon
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Asymmetric Flow Field-Flow Fractionation Investigation of Magnetopolyplexes
Macromolecular Chemistry and Physics, 2015Asymmetric flow field-flow fractionation (AF4) is an analytical separation technique capable of providing information on different parameters of complex systems in a single measurement. Although it shows promise to be an important tool in the field of gene therapy, AF4 has only occasionally been used to study gene delivery vectors such as polyplexes ...
Haladjova E. +6 more
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Miniaturized asymmetrical flow field‐flow fractionation: Application to biological vesicles
Journal of Separation Science, 2007AbstractAsymmetrical flow field‐flow fractionation (AFlFFF) has been carried out in a miniaturized channel by reducing the channel dimensions. Performance of the miniaturized AFlFFF (mAFlFFF) channel was evaluated with standard proteins and polystyrene latex spheres from nanometer to micrometer size.
Oh, Sunok. +5 more
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Present situation and development trends of asymmetrical flow field-flow fractionation
Chinese Journal of Chromatography, 2017Field-flow fractionation (FFF) is a kind of mature separation technologies in the field of bioanalysis, feasible of separating analytes with the differences of certain physical and chemical properties by the combination effects of two orthogonal force fields (flow field and external force field).
Qihui, Liang +3 more
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Optimization of asymmetrical flow field-flow fractionation
LC GC North America, 2011Asymmetrical flow field-flow fractionation can be used on a routine basis to separate high molecular weight compounds as an alternative to size-exclusion chromatography (SEC), but the principle of separation is very different and a different approach is required for optimization.
Qureshi, R.N., Kok, W.T.
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Circular Asymmetrical Flow Field-Flow Fractionation for the Semipreparative Separation of Particles
Analytical Chemistry, 2003A new technique for the separation and characterization of particles and polymers based on asymmetrical flow field-flow fractionation was developed. The new circular asymmetrical flow field-flow fractionation instrument (CAFFFE) resembles a quasi-parallel arrangement of 12 individual flow channels.
Michael, Maskos, Wolfgang, Schupp
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Optimization and evaluation of asymmetric flow field-flow fractionation of silver nanoparticles
Journal of Chromatography A, 2013Asymmetric flow field-flow fractionation (AF(4)) in combination with on-line optical detection and mass spectrometry is one of the most promising methods for separation and quantification of nanoparticles (NPs) in complex matrices including food. However, to obtain meaningful results regarding especially the NP size distribution a number of parameters ...
Katrin Loeschner +7 more
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