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Asymmetrical flow field-flow fractionation for the analysis of PEG-asparaginase

Talanta, 2016
Monomethoxypolyethylene glycol L-asparaginase (PEG-ASNASE) is the PEGylated version of the enzyme L-asparaginase (ASNASE). Both are used for remission induction in acute lymphoblastic leukemia (ALL) and non-Hodgkin's lymphoma (NHL). The treatment control is generally carried out by performing activity assays, though methods to determine the actual ...
C, John   +4 more
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Cytochrome c?dimyristoylphosphatidylglycerol interactions studied by asymmetrical flow field-flow fractionation

Analytical and Bioanalytical Chemistry, 2004
Lipid membranes are well recognized ligands that bind peripheral and integral proteins in a specific manner and regulate their function. Cytochrome c (cyt c) is one of the partner peripheral protein that binds to the lipid membranes via electrostatic and hydrophobic interactions.
Gebrenegus, Yohannes   +4 more
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Flow optimisations with increased channel thickness in asymmetrical flow field-flow fractionation

Journal of Chromatography A, 2018
Retention in flow field-flow fractionation (flow FFF) is generally governed by the combination of crossflow and migration flowrates. Especially for an asymmetrical flow FFF (AF4) channel in which the channel-inlet flow is divided into crossflow and outflow, the separation of low-molecular-weight proteins or macromolecules requires a relatively high ...
Joon Seon Yang, Myeong Hee Moon
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Optimization of asymmetrical flow field-flow fractionation (AF4)

LC GC Europe, 2010
Asymmetrical flow field-flow fractionation (AF4) can be used on a routine basis to separate high-molecular-weight compounds as an alternative to size-exclusion chromatography (SEC), but the principle of separation is very different and a different approach is required for optimization.
Qureshi, R.N., Kok, W.T.
openaire   +1 more source

Retention ratio and nonequilibrium bandspreading in asymmetrical flow field-flow fractionation

Analytical and Bioanalytical Chemistry, 2015
In asymmetrical flow field-flow fractionation (As-FlFFF), only the membrane-covered accumulation wall is permeable to fluid; the opposite channel wall is impermeable. Fluid enters the channel at the inlet and exits partly through the membrane-covered accumulation wall and partly through the channel outlet.
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Field-Flow Fractionation with Asymmetrical Electroosmotic Flow. II. Charged Particles

Journal of Liquid Chromatography & Related Technologies, 1997
The study of field-flow fractionation (FFF) with asymmetrical electroosmotically driven flow is extended to the case of charged sample particles. In this case, field-flow fractionation is combined with capillary zone electrophoresis in the same flat FFF channel.
V. P. Andreev, Y. V. Stepanov
openaire   +1 more source

Asymmetric Field-Flow Fractionation in Biotechnology

2005
Thorsten Klein, Christine Hürzeler
openaire   +1 more source

Characterization of hydrocolloids by asymmetric flow field-flow fractionation

Proceedings of the 10th international conference on photoacoustic and photothermal phenomena, 1999
A. Exner   +4 more
openaire   +1 more source

Separation of Macromolecules by Miniaturized Asymmetrical Flow Field Flow Fractionation

2013
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Kralova, J.   +4 more
openaire   +1 more source

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