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Aptamer–protein binding detected by asymmetric flow field flow fractionation
Journal of Chromatography A, 2013Asymmetric flow field flow fractionation (AF4) should be suitable for the study of aptamer-target binding, because its gentle separation would impose little disturbance to the complex structure, and it can use carrier solutions with high salt concentrations to provide the most optimal interaction environment to the complex.
Samantha, Schachermeyer +2 more
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Analytical and Bioanalytical Chemistry, 2019
Dynamic light scattering (DLS), transmission electron microscopy (TEM), and reversed phase-high performance liquid chromatography (RP-HPLC) are staples of nanoparticle characterization for size distribution, shape/morphology, and composition, respectively. These techniques are simple and provide important details on sample characteristics. However, DLS
Yingwen, Hu +2 more
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Dynamic light scattering (DLS), transmission electron microscopy (TEM), and reversed phase-high performance liquid chromatography (RP-HPLC) are staples of nanoparticle characterization for size distribution, shape/morphology, and composition, respectively. These techniques are simple and provide important details on sample characteristics. However, DLS
Yingwen, Hu +2 more
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Size Characterization and Quantification of Exosomes by Asymmetrical-Flow Field-Flow Fractionation
Analytical Chemistry, 2015In the past few years extracellular vesicles called exosomes have gained huge interest of scientific community since they show a great potential for human diagnostic and therapeutic applications. However, an ongoing challenge is accurate size characterization and quantification of exosomes because of the lack of reliable characterization techniques. In
Simona, Sitar +6 more
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Retention ratio and nonequilibrium bandspreading in asymmetrical flow field-flow fractionation
Analytical and Bioanalytical Chemistry, 2015In asymmetrical flow field-flow fractionation (As-FlFFF), only the membrane-covered accumulation wall is permeable to fluid; the opposite channel wall is impermeable. Fluid enters the channel at the inlet and exits partly through the membrane-covered accumulation wall and partly through the channel outlet.
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Flow optimisations with increased channel thickness in asymmetrical flow field-flow fractionation
Journal of Chromatography A, 2018Retention in flow field-flow fractionation (flow FFF) is generally governed by the combination of crossflow and migration flowrates. Especially for an asymmetrical flow FFF (AF4) channel in which the channel-inlet flow is divided into crossflow and outflow, the separation of low-molecular-weight proteins or macromolecules requires a relatively high ...
Joon Seon Yang, Myeong Hee Moon
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Characterization of Polymer Nanoparticles by Asymmetrical Flow Field Flow Fractionation (AF-FFF)
Journal of Nanoscience and Nanotechnology, 2010We present the characterization of different polymeric nanoparticles with asymmetrical flow field-flow fractionation (AF-FFF) in different solvents and additional, independent methods such as static and dynamic light scattering (SLS, DLS) in solution and transmission electron microscopy (TEM) and atomic force microscopy (AFM) for the visualization of ...
Christian, Scherer +6 more
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Asymmetric Field-Flow Fractionation in Biotechnology
2005Thorsten Klein, Christine Hürzeler
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Characterization of hydrocolloids by asymmetric flow field-flow fractionation
Proceedings of the 10th international conference on photoacoustic and photothermal phenomena, 1999A. Exner +4 more
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