Results 231 to 240 of about 1,899 (278)
Characterisation of polymers and particles by asymmetrical flow field-flow fractionation
, 2002 openaire +1 more source
Optimisation of asymmetrical flow field flow fractionation for environmental nanoparticles separation [PDF]
Journal of Chromatography A, 2008 The fractionation of natural nanoparticles by Asymmetrical Flow Field Flow Fractionation (As-Fl-FFF) was optimised by considering the following operating conditions: ionic strength, surfactant concentration and crossflow rate. The method performances such as fractionation recovery and fractionation efficiency were evaluated on a stable solution of ...
Gaétane Lespes +2 more
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Asymmetrical flow field-flow-fractionation in pharmaceutical analytics [PDF]
, 2003 Due to enormous progress in recombinant DNA techniques and methodology, a multitude of biosynthetic, pharmaceutically relevant polypeptides and proteins became available in the past decade and have been employed in numerous pharmaceutical products. Concomitantly, substantial progress was made in pharmaceutical formulation development of peptides and ...
Fraunhofer, Wolfgang
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Optimization of asymmetrical flow field-flow fractionation (AF4) [PDF]
LC GC Europe, 2010 Asymmetrical flow field-flow fractionation (AF4) can be used on a routine basis to separate high-molecular-weight compounds as an alternative to size-exclusion chromatography (SEC), but the principle of separation is very different and a different approach is required for optimization.
Qureshi, R.N., Kok, W.T.
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Field and flow programming in frit-inlet asymmetrical flow field-flow fractionation
Journal of Chromatography A, 2002The separation of wide molecular mass (Mr) ranges of macromolecules using frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF) has been improved by implementing a combination of field and flow programming. In this first implementation, field strength (governed by the cross flow-rate through the membrane-covered accumulation wall) is ...
Myeong Moon +2 more
exaly +3 more sources
Characterization of enzymatically synthesized amylopectin analogs via asymmetrical flow field flow fractionation [PDF]
Polymer, 2014 Asymmetrical flow field flow fractionation (AF4), when coupled with multi-angle laser light scattering (MALLS), is a very powerful technique for determination of the macromolecular structure of high molar mass (branched) polysaccharides.
Katja Loos, Agnes Rolland-Sabaté
exaly +2 more sources
Asymmetric Flow Field-Flow Fractionation in the Field of Nanomedicine
Analytical Chemistry, 2014Asymmetric flow field-flow fractionation (AF4) is a widely used and versatile technique in the family of field-flow fractionations, indicated by a rapidly increasing number of publications. It represents a gentle separation and characterization method, where nonspecific interactions are reduced to a minimum, allows a broad separation range from several
Michael, Wagner +4 more
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Analysis of plant ribosomes with asymmetric flow field-flow fractionation
Analytical and Bioanalytical Chemistry, 2013Ribosome profiling is a technique used to separate ribosomal subunits, 80S ribosomes (monosomes), and polyribosomes (polysomes) from other RNA-protein complexes. It is traditionally performed in sucrose gradients. In this study, we used asymmetric flow field-flow fractionation (AsFlFFF) to characterize ribosome profiles of Nicotiana benthamiana plants.
Eskelin Katri +2 more
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Analysis of Peptides using Asymmetrical Flow Field-flow Fractionation (AF4)
Journal of Pharmaceutical Sciences, 2021While asymmetrical flow field-flow fractionation (AF4) has been widely used for separation of high molecular weight species and even particles, its ability to resolve lower molecular weight species has rarely been explored. Over the course of many projects, we have discovered that AF4 can be an effective analytical method for separating peptides from ...
Ryan R, Manning +6 more
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Fractionation of Humic Substances by Asymmetrical Flow Field-Flow Fractionation
Chromatographia, 2005Asymmetrical flow field-flow fractionation was used to investigate the effects of pH and ionic strength of the buffer, and the binding of polyaromatic hydrocarbons (PAHs) on the particle sizes of humic substances (HSs). Particle sizes were greater when HSs were present in acidic medium with phosphate buffer than when they were present in alkaline ...
G. Yohannes +3 more
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