Results 231 to 240 of about 8,027 (278)

A method for efficient separation of polystyrene nanoplastics and its application in natural freshwater. [PDF]

RSC Adv
Ren P, Luo S, Wang L, Chi Y, Tang Y.
europepmc   +1 more source

Advanced Quality and Comparability Assessment of mRNA-Loaded Lipid Nanoparticles: Absolute Size Distribution Profiles and Structure from AF4-Coupled Light and X-ray Scattering Measurements. [PDF]

Anal Chem
Kolb B, Graewert M, Drexel R, Meier F, Raab J, Wilhelmy C, Nawroth T, Soloviov D, Haas H, Langguth P.   +9 more
europepmc   +1 more source

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Analysis of Peptides using Asymmetrical Flow Field-flow Fractionation (AF4)

Journal of Pharmaceutical Sciences, 2021
While asymmetrical flow field-flow fractionation (AF4) has been widely used for separation of high molecular weight species and even particles, its ability to resolve lower molecular weight species has rarely been explored. Over the course of many projects, we have discovered that AF4 can be an effective analytical method for separating peptides from ...
Ryan R, Manning, Ryan E, Holcomb, Derrick S, Katayama, Robert W, Payne, Joshua M, Stillahn, Charles S, Henry, Mark Cornell, Manning   +6 more
openaire   +2 more sources

Miniaturized asymmetrical flow field-flow fractionation: Application to biological vesicles

Journal of Separation Science, 2007
AbstractAsymmetrical flow field‐flow fractionation (AFlFFF) has been carried out in a miniaturized channel by reducing the channel dimensions. Performance of the miniaturized AFlFFF (mAFlFFF) channel was evaluated with standard proteins and polystyrene latex spheres from nanometer to micrometer size.
Dukjin Kang, Sung-Min Ahn, Myeong Hee Moon   +2 more
exaly   +3 more sources

Miniaturization of Frit Inlet Asymmetrical Flow Field-Flow Fractionation

Analytical Chemistry, 2004
A miniaturized frit inlet asymmetrical flow field-flow fractionation (mFI-AFlFFF) channel has been constructed and tested for the separation of proteins. By scaling down the geometrical channel dimension of a conventional FI-AFlFFF system, flow rate ranges that can be manipulated were decreased to 20-30 microL/min, which reduces the injection amount of
Dukjin, Kang, Myeong Hee, Moon
openaire   +2 more sources

Asymmetric Flow Field-Flow Fractionation Investigation of Magnetopolyplexes

Macromolecular Chemistry and Physics, 2015
Asymmetric flow field-flow fractionation (AF4) is an analytical separation technique capable of providing information on different parameters of complex systems in a single measurement. Although it shows promise to be an important tool in the field of gene therapy, AF4 has only occasionally been used to study gene delivery vectors such as polyplexes ...
Haladjova E., Rangelov S., Geisler M., Boye S., Lederer A., Mountrichas G., Pispas, Stergios   +6 more
openaire   +2 more sources

Optimisation of asymmetrical flow field flow fractionation for environmental nanoparticles separation

Journal of Chromatography A, 2008
The fractionation of natural nanoparticles by Asymmetrical Flow Field Flow Fractionation (As-Fl-FFF) was optimised by considering the following operating conditions: ionic strength, surfactant concentration and crossflow rate. The method performances such as fractionation recovery and fractionation efficiency were evaluated on a stable solution of ...
Frank von der Kammer, Isabelle Le Hécho, Gaëtane Lespes   +2 more
exaly   +3 more sources

Fractionation of Humic Substances by Asymmetrical Flow Field-Flow Fractionation

Chromatographia, 2005
Asymmetrical flow field-flow fractionation was used to investigate the effects of pH and ionic strength of the buffer, and the binding of polyaromatic hydrocarbons (PAHs) on the particle sizes of humic substances (HSs). Particle sizes were greater when HSs were present in acidic medium with phosphate buffer than when they were present in alkaline ...
G. Yohannes, S. K. Wiedmer, M. Jussila, M.-L. Riekkola   +3 more
openaire   +1 more source

Field and flow programming in frit-inlet asymmetrical flow field-flow fractionation

Journal of Chromatography A, 2002
The separation of wide molecular mass (Mr) ranges of macromolecules using frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF) has been improved by implementing a combination of field and flow programming. In this first implementation, field strength (governed by the cross flow-rate through the membrane-covered accumulation wall) is ...
Myeong Hee Moon, P Stephen Williams
exaly   +4 more sources

Distinguishing nanoparticle drug release mechanisms by asymmetric flow field–flow fractionation

Journal of Controlled Release, 2022
This research demonstrates the development, application, and mechanistic value of a multi-detector asymmetric flow field-flow fractionation (AF4) approach to acquire size-resolved drug loading and release profiles from polymeric nanoparticles (NPs). AF4 was hyphenated with multiple online detectors, including dynamic and multi-angle light scattering ...
Sheyda, Shakiba, Saba, Shariati, Haoran, Wu, Carlos E, Astete, Rafael, Cueto, Elham H, Fini, Debora F, Rodrigues, Cristina M, Sabliov, Stacey M, Louie   +8 more
openaire   +2 more sources