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Optimisation of asymmetrical flow field flow fractionation for environmental nanoparticles separation

Journal of Chromatography A, 2008
The fractionation of natural nanoparticles by Asymmetrical Flow Field Flow Fractionation (As-Fl-FFF) was optimised by considering the following operating conditions: ionic strength, surfactant concentration and crossflow rate. The method performances such as fractionation recovery and fractionation efficiency were evaluated on a stable solution of ...
S, Dubascoux   +4 more
openaire   +2 more sources

Cytochrome c?dimyristoylphosphatidylglycerol interactions studied by asymmetrical flow field-flow fractionation

Analytical and Bioanalytical Chemistry, 2004
Lipid membranes are well recognized ligands that bind peripheral and integral proteins in a specific manner and regulate their function. Cytochrome c (cyt c) is one of the partner peripheral protein that binds to the lipid membranes via electrostatic and hydrophobic interactions.
Gebrenegus, Yohannes   +4 more
openaire   +2 more sources

Flow optimisations with increased channel thickness in asymmetrical flow field-flow fractionation

Journal of Chromatography A, 2018
Retention in flow field-flow fractionation (flow FFF) is generally governed by the combination of crossflow and migration flowrates. Especially for an asymmetrical flow FFF (AF4) channel in which the channel-inlet flow is divided into crossflow and outflow, the separation of low-molecular-weight proteins or macromolecules requires a relatively high ...
Joon Seon Yang, Myeong Hee Moon
openaire   +2 more sources

Optimization of asymmetrical flow field-flow fractionation (AF4)

LC GC Europe, 2010
Asymmetrical flow field-flow fractionation (AF4) can be used on a routine basis to separate high-molecular-weight compounds as an alternative to size-exclusion chromatography (SEC), but the principle of separation is very different and a different approach is required for optimization.
Qureshi, R.N., Kok, W.T.
openaire   +1 more source

Retention ratio and nonequilibrium bandspreading in asymmetrical flow field-flow fractionation

Analytical and Bioanalytical Chemistry, 2015
In asymmetrical flow field-flow fractionation (As-FlFFF), only the membrane-covered accumulation wall is permeable to fluid; the opposite channel wall is impermeable. Fluid enters the channel at the inlet and exits partly through the membrane-covered accumulation wall and partly through the channel outlet.
openaire   +2 more sources

Field-Flow Fractionation with Asymmetrical Electroosmotic Flow. II. Charged Particles

Journal of Liquid Chromatography & Related Technologies, 1997
The study of field-flow fractionation (FFF) with asymmetrical electroosmotically driven flow is extended to the case of charged sample particles. In this case, field-flow fractionation is combined with capillary zone electrophoresis in the same flat FFF channel.
V. P. Andreev, Y. V. Stepanov
openaire   +1 more source

Factors affecting measurement of channel thickness in asymmetrical flow field-flow fractionation

Journal of Chromatography A, 2015
Asymmetrical flow field-flow fractionation (AF4) has been considered to be a useful tool for simultaneous separation and characterization of polydisperse macromolecules or colloidal nanoparticles. AF4 analysis requires the knowledge of the channel thickness (w), which is usually measured by injecting a standard with known diffusion coefficient (D) or ...
Haiyang, Dou   +2 more
openaire   +2 more sources

Asymmetrical flow field-flow fractionation combined with ultrafiltration: A novel and high-efficiency approach for separation, purification, and characterization of Ganoderma lucidum polysaccharides

Talanta: The International Journal of Pure and Applied Analytical Chemistry, 2022
Yuxi Guo   +5 more
semanticscholar   +1 more source

Asymmetrical Flow Field Flow Fractionation for Molar Mass Characterization of Polyethylene Oxide in Abuse-Deterrent Formulations.

Journal of Chromatography A, 2023
Haiou Qu   +6 more
semanticscholar   +1 more source

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