Results 41 to 50 of about 35,272 (308)

Screening for protein–protein interactions with asymmetrical flow field-flow fractionation [PDF]

open access: yesJournal of Pharmaceutical Sciences, 2021
We describe a new method for screening protein-protein interaction of biopharmaceutical molecules at dilute concentrations to predict development issues at high concentration. The method is based on Asymmetrical Flow Field-Flow Fractionation (AF4) measurements using well known effects of protein-protein attraction on the fractionation profile due to ...
Per-Olof, Wahlund   +2 more
openaire   +2 more sources

Aqueous Dilution of Noble NPs Bulk Dispersions: Modeling Instability due to Dissolution by AF4 and Stablishing Considerations for Plasmonic Assays

open access: yesNanomaterials, 2020
Among different nanomaterials, gold and silver nanoparticles (AuNPs and AgNPs) have become useful tools for a wide variety of applications in general, and particularly for plasmonic assays.
Lorenzo Sanjuan-Navarro   +3 more
doaj   +1 more source

The Power of Field-Flow Fractionation in Characterization of Nanoparticles in Drug Delivery

open access: yesMolecules, 2023
Asymmetric-flow field-flow fractionation (AF4) is a gentle, flexible, and powerful separation technique that is widely utilized for fractionating nanometer-sized analytes, which extend to many emerging nanocarriers for drug delivery, including lipid ...
Juan Bian   +3 more
doaj   +1 more source

Field-Flow Fractionation in Molecular Biology and Biotechnology

open access: yesMolecules, 2023
Field-flow fractionation (FFF) is a family of single-phase separative techniques exploited to gently separate and characterize nano- and microsystems in suspension.
Stefano Giordani   +5 more
doaj   +1 more source

A new way of valorizing biomaterials: the use of sunflower protein for 1 a-tocopherol microencapsulation [PDF]

open access: yes, 2013
Biopolymer based microparticles were efficiently prepared from sunflower protein (SP) wall material and a-tocopherol (T) active core using a spray-drying technique. Protein enzymatic hydrolysis and/or N-acylation were carried out to make some structural
A. Nesterenko   +64 more
core   +4 more sources

Characterization of aggregates of surface modified fullerenes by asymmetrical flow field-flow fractionation with multi-angle light scattering detection [PDF]

open access: yes, 2016
Fullerenes are carbon nanoparticles with widespread biomedical, commercial and industrial applications. Attributes such as their tendency to aggregate and aggregate size and shape impact their ability to be transported into and through the environment ...
Astefanei, Alina   +6 more
core   +1 more source

Single Cell-Asymmetrical Flow Field-Flow Fractionation/ICP-Time of Flight-Mass Spectrometry (sc-AF4/ICP-ToF-MS): An efficient alternative for the cleaning and multielemental analysis of individual cells

open access: yesJournal of Analytical Atomic Spectrometry, 2022
One of the greatest challenges in the analysis of single cells, quite often is the severe ionic background their samples exhibit.
Michail-Ioannis Chronakis   +2 more
semanticscholar   +1 more source

A general perspective of the characterization and quantification of nanoparticles: Imaging, spectroscopic, and separation techniques [PDF]

open access: yes, 2014
This article gives an overview of the different techniques used to identify, characterize, and quantify engineered nanoparticles (ENPs). The state-of-the-art of the field is summarized, and the different characterization techniques have been grouped ...
Anderson de la LLana, S.   +10 more
core   +3 more sources

Comparison of Miniaturized and Conventional Asymmetrical Flow Field-Flow Fractionation (AF4) Channels for Nanoparticle Separations

open access: yesSeparations, 2017
The performance of a miniaturized channel for the separation of polymer and metal nanoparticles (NP) using Asymmetrical Flow Field-Flow Fractionation (AF4) was investigated and compared with a conventional AF4 system.
Zengchao You   +2 more
doaj   +1 more source

Size and optical properties of dissolved organic matter in large boreal rivers during mixing: Implications for carbon transport and source discrimination

open access: yesJournal of Hydrology: Regional Studies, 2022
Study region: The lower Athabasca River (LAR), Northern Alberta, Canada Study focus: The functionality of dissolved organic matter (DOM) in natural waters largely depends upon its size and composition.
Jin-Ping Xue   +7 more
doaj   +1 more source

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