Results 21 to 30 of about 10,369,778 (272)

Optimization of asymmetrical flow field-flow fractionation

open access: yesLC GC North America, 2011
Asymmetrical flow field-flow fractionation can be used on a routine basis to separate high molecular weight compounds as an alternative to size-exclusion chromatography (SEC), but the principle of separation is very different and a different approach is ...
Qureshi, R.N., Kok, W.T.
core   +2 more sources

A comprehensive analysis in one run - in-depth conformation studies of protein-polymer chimeras by asymmetrical flow field-flow fractionation. [PDF]

open access: yesChem Sci, 2021
CITATION: Kaupbayeva, B. et al. 2021. A comprehensive analysis in one run – in-depth conformation studies of protein–polymer chimeras by asymmetrical flow field-flow fractionation. Chemical Science 12(2021):9 pages.
Kaupbayeva B   +5 more
europepmc   +2 more sources

Key Methodologies in Characterizing the Multi-Scale Structures of Gluten Proteins in Dough: A Comparative Review [PDF]

open access: yesBiomolecules
Gluten proteins are key components in wheat flour that determine the formation of dough and the quality of flour-based products. Upon hydration and mixing, gluten proteins undergo complex structural transformations to form a gluten network, exhibiting a ...
Feifei Su   +6 more
doaj   +2 more sources

Physicochemical characterization and study of molar mass of industrial gelatins by AsFlFFF-UV/MALS and chemometric approach. [PDF]

open access: yesPLoS ONE, 2018
Industrial gelatins have different physicochemical properties that mainly depend of the raw materials origin and the extraction conditions. These properties are closely related to the molar mass distribution of these gelatins.
Simon Duthen   +6 more
doaj   +1 more source

Asymmetric flow field‐flow fractionation of liposomes: optimization of fractionation variables [PDF]

open access: yesJournal of Separation Science, 2009
AbstractThe purpose of this study was to investigate the influence of ionic strength of the carrier liquid, cross flow rate, focus flow rate, and sample load on the retention behavior of liposomes in asymmetric flow field‐flow fractionation (AF4). Two differently prepared samples of large unilamellar vesicles (LUV) were used. Experiments were performed
Stefan, Hupfeld   +2 more
openaire   +2 more sources

Revisiting the dynamics of proteins during milk powder hydration using asymmetric flow field-flow fractionation (AF4)

open access: yesCurrent Research in Food Science, 2021
The dynamics of β-casein and casein micelles in the reconstitution of skim milk were revisited in this study. β-casein migrates into casein micelles upon an increase in temperatures due to an increase in the hydrophobic effect and lower calcium-phosphate
Anouk Lie-Piang   +4 more
doaj   +1 more source

Revision of the calibration experiment in asymmetrical flow field-flow fractionation

open access: yesJournal of Chromatography A, 2021
Asymmetrical flow field-flow fractionation is a versatile chromatographic fractionation method. In combination with at least one detection technique it is used for size-based separation of colloids, biomolecules and polymers. Although often used as pure separation method, a well-elaborated theory is available that allows precise quantification of the ...
Häusele, Benedikt   +2 more
openaire   +3 more sources

Recovery, overloading, and protein interactions in asymmetrical flow field-flow fractionation. [PDF]

open access: yesAnal Bioanal Chem, 2019
In asymmetrical flow field-flow fractionation (AF4), similar to other separation techniques, mass recovery and overloading require special attention in order to obtain quantitative results. We conducted a systematic study with five globular proteins of different molecular weight (36.7-669 kDa) and isoelectric point (4.0-6.5), and ultrafiltration ...
Marioli M, Kok WT.
europepmc   +6 more sources

Asymmetric flow field flow fractionation methods for virus purification [PDF]

open access: yesJournal of Chromatography A, 2016
Detailed biochemical and biophysical characterization of viruses requires viral preparations of high quantity and purity. The optimization of virus production and purification is an essential, but laborious and time-consuming process. Asymmetric flow field flow fractionation (AF4) is an attractive alternative method for virus purification because it is
Eskelin, Katri Johanna   +5 more
openaire   +3 more sources

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