Results 231 to 240 of about 10,369,778 (272)

Optimisation of asymmetrical flow field flow fractionation for environmental nanoparticles separation

Journal of Chromatography A, 2008
The fractionation of natural nanoparticles by Asymmetrical Flow Field Flow Fractionation (As-Fl-FFF) was optimised by considering the following operating conditions: ionic strength, surfactant concentration and crossflow rate. The method performances such as fractionation recovery and fractionation efficiency were evaluated on a stable solution of ...
Gaëtane Lespes, Frank von der Kammer, Isabelle Le Hécho   +2 more
exaly   +3 more sources

Fractionation of Humic Substances by Asymmetrical Flow Field-Flow Fractionation

Chromatographia, 2005
Asymmetrical flow field-flow fractionation was used to investigate the effects of pH and ionic strength of the buffer, and the binding of polyaromatic hydrocarbons (PAHs) on the particle sizes of humic substances (HSs). Particle sizes were greater when HSs were present in acidic medium with phosphate buffer than when they were present in alkaline ...
G. Yohannes, S. K. Wiedmer, M. Jussila, M.-L. Riekkola   +3 more
openaire   +1 more source

Miniaturized asymmetrical flow field-flow fractionation: Application to biological vesicles

Journal of Separation Science, 2007
AbstractAsymmetrical flow field‐flow fractionation (AFlFFF) has been carried out in a miniaturized channel by reducing the channel dimensions. Performance of the miniaturized AFlFFF (mAFlFFF) channel was evaluated with standard proteins and polystyrene latex spheres from nanometer to micrometer size.
Richard J. Simpson, Sung-Min Ahn, Myeong Hee Moon   +2 more
exaly   +3 more sources

Miniaturization of Frit Inlet Asymmetrical Flow Field-Flow Fractionation

Analytical Chemistry, 2004
A miniaturized frit inlet asymmetrical flow field-flow fractionation (mFI-AFlFFF) channel has been constructed and tested for the separation of proteins. By scaling down the geometrical channel dimension of a conventional FI-AFlFFF system, flow rate ranges that can be manipulated were decreased to 20-30 microL/min, which reduces the injection amount of
Dukjin, Kang, Myeong Hee, Moon
openaire   +2 more sources

Fractionation of Prion Protein Aggregates by Asymmetrical Flow Field‐Flow Fractionation

2006
Achieving the successful separation and analysis of amyloid and other large protein aggregates can be a difficult proposition. Field-flow fractionation (FFF) is a flow-based separation method like chromatography; however, FFF is capable of high-resolution separations in the absence of a stationary matrix.
Jay R, Silveira, Andrew G, Hughson, Byron, Caughey   +2 more
openaire   +2 more sources

The use of asymmetrical flow field-flow fractionation in pharmaceutics and biopharmaceutics

European Journal of Pharmaceutics and Biopharmaceutics, 2004
Field-flow fractionation (FFF) is a family of flexible analytical fractionating techniques which have the advantage that the separation of analytes is achieved, solely through the interaction of the sample with an external, perpendicular physical field, rather than by the interaction with a stationary phase.
Wolfgang, Fraunhofer, Gerhard, Winter
openaire   +2 more sources

Characterization of enzymatically synthesized amylopectin analogs via asymmetrical flow field flow fractionation

Polymer, 2014
Asymmetrical flow field flow fractionation (AF4), when coupled with multi-angle laser light scattering (MALLS), is a very powerful technique for determination of the macromolecular structure of high molar mass (branched) polysaccharides.
Katja Loos, Agnès Rolland-Sabaté
exaly   +2 more sources

Asymmetrical flow field-flow fractionation for the analysis of PEG-asparaginase

Talanta, 2016
Monomethoxypolyethylene glycol L-asparaginase (PEG-ASNASE) is the PEGylated version of the enzyme L-asparaginase (ASNASE). Both are used for remission induction in acute lymphoblastic leukemia (ALL) and non-Hodgkin's lymphoma (NHL). The treatment control is generally carried out by performing activity assays, though methods to determine the actual ...
C, John, T, Herz, J, Boos, K, Langer, G, Hempel   +4 more
openaire   +2 more sources

Field and flow programming in frit-inlet asymmetrical flow field-flow fractionation

Journal of Chromatography A, 2002
The separation of wide molecular mass (Mr) ranges of macromolecules using frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF) has been improved by implementing a combination of field and flow programming. In this first implementation, field strength (governed by the cross flow-rate through the membrane-covered accumulation wall) is ...
Myeong Hee Moon, Dukjin Kang, Philip Stephen Williams   +2 more
exaly   +3 more sources

Asymmetric Flow Field-Flow Fractionation Investigation of Magnetopolyplexes

Macromolecular Chemistry and Physics, 2015
Asymmetric flow field-flow fractionation (AF4) is an analytical separation technique capable of providing information on different parameters of complex systems in a single measurement. Although it shows promise to be an important tool in the field of gene therapy, AF4 has only occasionally been used to study gene delivery vectors such as polyplexes ...
Haladjova E., Rangelov S., Geisler M., Boye S., Lederer A., Mountrichas G., Pispas, Stergios   +6 more
openaire   +2 more sources