Fractionation of Nanoparticle Matter in Red Wines Using Asymmetrical Flow Field-Flow Fractionation. [PDF]
J Agric Food Chem, 2020 The particle matter of wine is mainly composed of wine colloids and macromolecules. The present work develops a methodology using asymmetrical flow field-flow fractionation coupled with multi-angle light scattering, differential refractive index detector, and ultraviolet detector (AsFlFFF-MALS-dRI-UV) for the fractionation and determination of the ...
Osorio-Macías DE +8 more
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Controlled Disassembly and Purification of Functional Viral Subassemblies Using Asymmetrical Flow Field-Flow Fractionation (AF4) [PDF]
Viruses, 2018 Viruses protect their genomes by enclosing them into protein capsids that sometimes contain lipid bilayers that either reside above or below the protein layer.
Katri Eskelin, Minna M. Poranen
doaj +4 more sources
Separations, 2017 The performance of a miniaturized channel for the separation of polymer and metal nanoparticles (NP) using Asymmetrical Flow Field-Flow Fractionation (AF4) was investigated and compared with a conventional AF4 system.
Zengchao You +2 more
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Fast and Purification-Free Characterization of Bio-Nanoparticles in Biological Media by Electrical Asymmetrical Flow Field-Flow Fractionation Hyphenated with Multi-Angle Light Scattering and Nanoparticle Tracking Analysis Detection [PDF]
Molecules, 2020 Accurate physico-chemical characterization of exosomes and liposomes in biological media is challenging due to the inherent complexity of the sample matrix.
Roland Drexel +5 more
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Revision of the calibration experiment in asymmetrical flow field-flow fractionation
Journal of Chromatography A, 2021 Asymmetrical flow field-flow fractionation is a versatile chromatographic fractionation method. In combination with at least one detection technique it is used for size-based separation of colloids, biomolecules and polymers. Although often used as pure separation method, a well-elaborated theory is available that allows precise quantification of the ...
Häusele, Benedikt +2 more
openaire +4 more sources
Characterization of Silver Nanoparticles under Environmentally Relevant Conditions Using Asymmetrical Flow Field-Flow Fractionation (AF4). [PDF]
PLoS ONE, 2015 The development of methods to monitor manufactured nanomaterials in the environment is one of the crucial areas for the assessment of their risk. More specifically, particle size analysis is a key element, because many properties of nanomaterial are size
Min-Hee Jang, Seungho Lee, Yu Sik Hwang
doaj +2 more sources
Characterization of binding between model protein GA-Z and human serum albumin using asymmetrical flow field-flow fractionation and small angle X-ray scattering. [PDF]
PLoS ONE, 2020 Protein-based drugs often require targeted drug delivery for optimal therapy. A successful strategy to increase the circulation time of the protein in the blood is to link the therapeutic protein with an albumin-binding domain.
Jaeyeong Choi +7 more
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Recovery, overloading, and protein interactions in asymmetrical flow field-flow fractionation. [PDF]
Anal Bioanal Chem, 2019 In asymmetrical flow field-flow fractionation (AF4), similar to other separation techniques, mass recovery and overloading require special attention in order to obtain quantitative results. We conducted a systematic study with five globular proteins of different molecular weight (36.7-669 kDa) and isoelectric point (4.0-6.5), and ultrafiltration ...
Marioli M, Kok WT.
europepmc +6 more sources
Asymmetrical flow field-flow fractionation and multi-angle laser light scattering: A new analytical approach for the characterisation of insect protein aggregation/polymerisation after heat treatment of Tenebrio molitor larvae [PDF]
Current Research in Food ScienceUnderstanding the structural modifications of insect proteins during the transformation processes used for extract preparation is essential for optimising their functionalities and obtaining high added-value proteins.
Ariel Anouma +3 more
doaj +2 more sources
Asymmetrical Flow Field-Flow Fractionation on Virus and Virus-Like Particle Applications. [PDF]
Microorganisms, 2019 Asymmetrical flow field-flow fractionation (AF4) separates sample components based on their sizes in the absence of a stationary phase. It is well suited for high molecular weight samples such as virus-sized particles. The AF4 experiment can potentially separate molecules within a broad size range (~103−109 Da; particle diameter from 2 nm to 0.5−1 μm).
Eskelin K, Poranen MM, Oksanen HM.
europepmc +5 more sources