Nanoparticle separation with a miniaturized asymmetrical flow field-flow fractionation cartridge [PDF]
Frontiers in Chemistry, 2015 Asymmetrical Flow Field-Flow Fractionation (AF4) is a separation technique applicable to particles over a wide size range. Despite the many advantages of AF4, its adoption in routine particle analysis is somewhat limited by the large footprint of ...
David eMüller +5 more
doaj +6 more sources
Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine [PDF]
Journal of Extracellular Vesicles, 2018 Extracellular vesicles (EVs) have a great potential in clinical applications. However, their isolation from different bodily fluids and their characterisation are currently not optimal or standardised.
Eline Oeyen +10 more
doaj +3 more sources
Recovery, overloading, and protein interactions in asymmetrical flow field-flow fractionation. [PDF]
Anal Bioanal Chem, 2019 In asymmetrical flow field-flow fractionation (AF4), similar to other separation techniques, mass recovery and overloading require special attention in order to obtain quantitative results. We conducted a systematic study with five globular proteins of different molecular weight (36.7-669 kDa) and isoelectric point (4.0-6.5), and ultrafiltration ...
Marioli M, Kok WT.
europepmc +9 more sources
Asymmetrical Flow Field-Flow Fractionation on Virus and Virus-Like Particle Applications. [PDF]
Microorganisms, 2019 Asymmetrical flow field-flow fractionation (AF4) separates sample components based on their sizes in the absence of a stationary phase. It is well suited for high molecular weight samples such as virus-sized particles. The AF4 experiment can potentially separate molecules within a broad size range (~103−109 Da; particle diameter from 2 nm to 0.5−1 μm).
Eskelin K, Poranen MM, Oksanen HM.
europepmc +11 more sources
Understanding the Degradation of Core-Shell Nanogels Using Asymmetrical Flow Field Flow Fractionation [PDF]
Journal of Functional Biomaterials, 2023 Nanogels are candidates for biomedical applications, and core-shell nanogels offer the potential to tune thermoresponsive behaviour with the capacity for extensive degradation.
Edyta Niezabitowska +5 more
doaj +2 more sources
Proteomics protocol for obtaining extracellular vesicle from human plasma using asymmetrical flow field-flow fractionation technology [PDF]
STAR Protocols, 2023 Summary: Plasma extracellular vesicles (EVs) represent a potential resource for biomarkers of multiple diseases. Here, we present a protocol for obtaining EVs from human plasma using asymmetrical flow field-flow fractionation technology.
Na Li +4 more
doaj +2 more sources
Native RNA Purification Method for Small RNA Molecules Based on Asymmetrical Flow Field-Flow Fractionation [PDF]
Pharmaceuticals, 2022 RNA molecules provide promising new possibilities for the prevention and treatment of viral infections and diseases. The rapid development of RNA biology and medicine requires advanced methods for the purification of RNA molecules, which allow fast and ...
Alesia A. Levanova +5 more
doaj +2 more sources
Study of the Stability of Citrate Capped AgNPs in Several Environmental Water Matrices by Asymmetrical Flow Field Flow Fractionation [PDF]
Nanomaterials, 2021 Asymmetrical flow field-flow fractionation (AF4) coupled to UV-Vis and dynamic light scattering (DLS) detectors in series, was tested for stability studies of dispersions of citrate-capped silver nanoparticles (AgNPs) in several water matrices.
Aaron Boughbina-Portolés +3 more
doaj +2 more sources
Quantitative size-resolved characterization of mRNA nanoparticles by in-line coupling of asymmetrical-flow field-flow fractionation with small angle X-ray scattering [PDF]
Scientific Reports, 2023 We present a generically applicable approach to determine an extensive set of size-dependent critical quality attributes inside nanoparticulate pharmaceutical products. By coupling asymmetrical-flow field-flow fractionation (AF4) measurements directly in-
Melissa A. Graewert +14 more
doaj +2 more sources
Assessment of the Effects of Structural Modification of Gastrodia elata Polysaccharide on Anti-Breast Cancer Activity Using Asymmetrical Flow Field-Flow Fractionation [PDF]
Molecules, 2023 Gastrodia elata (“Tian Ma” in Chinese) is used as a food and medical ingredient in traditional Chinese medicine. In this study, to enhance the anti-breast cancer activity of Gastrodia elata polysaccharide (GEP), GEPs were modified via sulfidation (SGEP ...
Xiaoying Liu +7 more
doaj +2 more sources