Analysis of Silicon Quantum Dots and Serum Proteins Interactions Using Asymmetrical Flow Field-Flow Fractionation. [PDF]
Langmuir, 2023 Semiconductor nanocrystals or quantum dots (QDs) have gained significant attention in biomedical research as versatile probes for imaging, sensing, and therapies.
Nagarajan U +4 more
europepmc +2 more sources
Offline Coupling of Asymmetrical Flow Field-Flow Fractionation and Capillary Electrophoresis for Separation of Extracellular Vesicles. [PDF]
Anal Chem, 2022 Extracellular vesicles (EVs) play important roles in cell-to-cell communications and carry high potential as markers targeted in disease diagnosis, prognosis, and therapeutic development.
Gao Z, Hutchins Z, Li Z, Zhong W.
europepmc +2 more sources
Enhancing Extracellular Vesicle Analysis by Integration of Large-Volume Sample Stacking in Capillary Electrophoresis with Asymmetrical Flow Field-Flow Fractionation. [PDF]
Anal Chem, 2023 Extracellular vesicles (EVs) play important roles in cell-cell communication and pathological development. Cargo profiling for the EVs present in clinical specimens can provide valuable insights into their functions and help discover effective EV-based ...
Gao Z +4 more
europepmc +2 more sources
Characterizing Non-covalent Protein Complexes Using Asymmetrical Flow Field-Flow Fractionation On-Line Coupled to Native Mass Spectrometry. [PDF]
Anal Chem, 2023 We report an online analytical platform based on the coupling of asymmetrical flow field-flow fractionation (AF4) and native mass spectrometry (nMS) in parallel with UV-absorbance, multi-angle light scattering (MALS), and differential-refractive-index ...
Ventouri IK +9 more
europepmc +2 more sources
Investigation of native and aggregated therapeutic proteins in human plasma with asymmetrical flow field-flow fractionation and mass spectrometry. [PDF]
Anal Bioanal Chem, 2022 Physiochemical degradation of therapeutic proteins in vivo during plasma circulation after administration can have a detrimental effect on their efficacy and safety profile. During drug product development, in vivo animal studies are necessary to explore
Ramm I +5 more
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CHIMIA, 2022 Asymmetrical flow field-flow fractionation (AF4) is a powerful technique employed for the separation of macromolecules, nanoparticles, and their assemblages according to their hydrodynamic behavior.
Isabelle A. M. Worms, Vera I. Slaveykova
doaj +2 more sources
A comprehensive study on the relationship between structure and antioxidant activity of natural deep eutectic solvent extracted polysaccharides from Chinese yam peel using asymmetrical flow field-flow fractionation [PDF]
Food Chemistry: XIn this study, an ultrasound-assisted natural deep eutectic solvents (NADES) combined with enzymatic deproteinization was developed for extracting Chinese Yam peel polysaccharide (CYPP) from two varieties.
Yao Huang +6 more
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Application of Asymmetrical Flow Field-Flow Fractionation for Characterizing the Size and Drug Release Kinetics of Theranostic Lipid Nanovesicles. [PDF]
Int J Mol Sci, 2021 Liposome size and in vitro release of the active substance belong to critical quality attributes of liposomal carriers. Here, we apply asymmetric flow field-flow fractionation (AF4) to characterize theranostic liposomes prepared by thin lipid film ...
Skupin-Mrugalska P, Elvang PA, Brandl M.
europepmc +2 more sources
Proteins and antibodies in serum, plasma, and whole blood-size characterization using asymmetrical flow field-flow fractionation (AF4). [PDF]
Anal Bioanal Chem, 2018 The analysis of aggregates of therapeutic proteins is crucial in order to ensure efficacy and patient safety. Typically, the analysis is performed in the finished formulation to ensure that aggregates are not present.
Leeman M +4 more
europepmc +2 more sources
Perturbations of Lipids and Oxidized Phospholipids in Lipoproteins of Patients with Postmenopausal Osteoporosis Evaluated by Asymmetrical Flow Field-Flow Fractionation and Nanoflow UHPLC–ESI–MS/MS [PDF]
Antioxidants, 2020 Osteoporosis, a degenerative bone disease characterized by reduced bone mass and high risk of fragility, is associated with the alteration of circulating lipids, especially oxidized phospholipids (Ox-PLs).
Kang Geun Lee +3 more
doaj +2 more sources