Results 31 to 40 of about 1,892 (276)

Field-Flow Fractionation in Molecular Biology and Biotechnology

Molecules, 2023
Field-flow fractionation (FFF) is a family of single-phase separative techniques exploited to gently separate and characterize nano- and microsystems in suspension.
Stefano Giordani, Valentina Marassi, Anna Placci, Andrea Zattoni, Barbara Roda, Pierluigi Reschiglian   +5 more
doaj   +1 more source

CE and asymmetrical flow field flow fractionation studies of polymer interactions with surfaces and solutes reveal conformation changes of polymers [PDF]

, 2020
Amphiphilic diblock copolymers consisting of a hydrophobic core containing a polymerized ionic liquid and an outer shell composed of poly(N-isoprolylacrylamide) were investigated by capillary electrophoresis and asymmetrical flow-field flow fractionation.
Tenhu, Heikki, Erno Karjalainen, Heikki Tenhu, Wiedmer, Susanne K., Joanna Witos, Witos, Joanna, Karjalainen, Erno; id_orcid, Susanne K. Wiedmer, Karjalainen, Erno   +8 more
core   +1 more source

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension [PDF]

Journal of Visualized Experiments, 2020
Particle size is arguably the most important physico-chemical parameter associated with the notion of a nanoparticle. Precise knowledge of the size and size distribution of nanoparticles is of utmost importance for various applications. The size range is also important, as it defines the most "active" component of a nanoparticle dose. Asymmetrical Flow
Drexel, Roland, Sogne, Vanessa, Dinkel, Magdalena, Meier, Florian, Klein, Thorsten   +4 more
openaire   +2 more sources

Screening for protein–protein interactions with asymmetrical flow field-flow fractionation [PDF]

Journal of Pharmaceutical Sciences, 2021
We describe a new method for screening protein-protein interaction of biopharmaceutical molecules at dilute concentrations to predict development issues at high concentration. The method is based on Asymmetrical Flow Field-Flow Fractionation (AF4) measurements using well known effects of protein-protein attraction on the fractionation profile due to ...
Per-Olof, Wahlund, Nikolai, Lorenzen, Christian, Rischel   +2 more
openaire   +2 more sources

Asymmetrical Flow Field-Flow Fractionation Coupled to ICP-MS for Characterization of Trace Metal Species in the Environment from Macromolecular to Nano-Assemblage Forms: Current Challenges for Quantification

CHIMIA, 2022
Asymmetrical flow field-flow fractionation (AF4) is a powerful technique employed for the separation of macromolecules, nanoparticles, and their assemblages according to their hydrodynamic behavior.
Isabelle A. M. Worms, Vera I. Slaveykova
doaj   +1 more source

Application of microstructured membranes for increasing retention, selectivity and resolution in asymmetrical flow field-flow fractionation [PDF]

, 2019
In the present proof-of-concept study, we demonstrate that retention time, selectivity and resolution can be increased in asymmetrical flow field-flow fractionation (AF4) by introducing microstructured ultrafiltration membranes.
Stamatialis, Dimitrios, Kavurt, Ü. Bade, Kok, Wim Th, Marioli, Maria   +3 more
core   +1 more source

Sample carryover and cleaning procedures for asymmetrical flow field-flow fractionation instrument [PDF]

, 2021
Asymmetrical flow field-flow (AF4) fractionation aims in separation of sample components to yield elution of homogenous fractions identified as well-defined peaks in the chromatograms.
Poranen, Minna, Eskelin , Katri, Oksanen, Hanna M   +2 more
core   +1 more source

Size and optical properties of dissolved organic matter in large boreal rivers during mixing: Implications for carbon transport and source discrimination

Journal of Hydrology: Regional Studies, 2022
Study region: The lower Athabasca River (LAR), Northern Alberta, Canada Study focus: The functionality of dissolved organic matter (DOM) in natural waters largely depends upon its size and composition.
Jin-Ping Xue, Chad W. Cuss, Tommy Noernberg, Muhammad B. Javed, Na Chen, Rick Pelletier, Yu Wang, William Shotyk   +7 more
doaj   +1 more source

Quality control and purification of ready-to-use conjugated gold nanoparticles to ensure effectiveness in biosensing

Frontiers in Sensors, 2022
Introduction: Gold nanoparticles (AuNPs) and their conjugates are used for many applications in the field of sensors. Literature lacks procedures able to separate, purify and characterize these species in native conditions without altering them while ...
Junjie Wang, Stefano Giordani, Valentina Marassi, Valentina Marassi, Barbara Roda, Barbara Roda, Pierluigi Reschiglian, Pierluigi Reschiglian, Andrea Zattoni, Andrea Zattoni   +9 more
doaj   +1 more source

Asymmetrical flow field-flow fractionation in purification of an enveloped bacteriophage ϕ6 [PDF]

Journal of Chromatography B, 2018
Basic and applied virus research requires specimens that are purified to high homogeneity. Thus, there is much interest in the efficient production and purification of viruses and their subassemblies. Advances in the production steps have shifted the bottle neck of the process to the purification. Nonetheless, the development of purification techniques
Lampi, Mirka, Oksanen, Hanna M., Meier, Florian, Moldenhauer, Evelin, Poranen, Minna M., Bamford, Dennis H., Eskelin, Katri   +6 more
openaire   +3 more sources