Characterization and Quantitation of Carbon Black Nanomaterials in Polymeric and Biological Aqueous Dispersants by Asymmetrical Flow Field Flow Fractionation [PDF]
ACS Omega, 2021 Lorenzo Sanjuan-Navarro +2 more
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Asymmetrical flow field-flow fractionation in purification of an enveloped bacteriophage ϕ6 [PDF]
Journal of Chromatography B, 2018 Basic and applied virus research requires specimens that are purified to high homogeneity. Thus, there is much interest in the efficient production and purification of viruses and their subassemblies. Advances in the production steps have shifted the bottle neck of the process to the purification. Nonetheless, the development of purification techniques
Lampi, Mirka +6 more
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Key Methodologies in Characterizing the Multi-Scale Structures of Gluten Proteins in Dough: A Comparative Review [PDF]
BiomoleculesGluten proteins are key components in wheat flour that determine the formation of dough and the quality of flour-based products. Upon hydration and mixing, gluten proteins undergo complex structural transformations to form a gluten network, exhibiting a ...
Feifei Su +6 more
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A One‐Step Workflow for Size‐Based Separation of Extracellular Vesicles With Integrated Surface Marker Detection [PDF]
Journal of Extracellular BiologyExtracellular vesicles (EVs) are released by diverse cell types in biofluids and are increasingly studied in liquid biopsies as diagnostic and prognostic biomarkers for various diseases, including cancer.
Lien Lippens +13 more
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Asymmetric flow field‐flow fractionation of liposomes: optimization of fractionation variables [PDF]
Journal of Separation Science, 2009 AbstractThe purpose of this study was to investigate the influence of ionic strength of the carrier liquid, cross flow rate, focus flow rate, and sample load on the retention behavior of liposomes in asymmetric flow field‐flow fractionation (AF4). Two differently prepared samples of large unilamellar vesicles (LUV) were used. Experiments were performed
Stefan, Hupfeld +2 more
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Current Research in Food Science, 2021 The dynamics of β-casein and casein micelles in the reconstitution of skim milk were revisited in this study. β-casein migrates into casein micelles upon an increase in temperatures due to an increase in the hydrophobic effect and lower calcium-phosphate
Anouk Lie-Piang +4 more
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Asymmetric flow field flow fractionation methods for virus purification [PDF]
Journal of Chromatography A, 2016 Detailed biochemical and biophysical characterization of viruses requires viral preparations of high quantity and purity. The optimization of virus production and purification is an essential, but laborious and time-consuming process. Asymmetric flow field flow fractionation (AF4) is an attractive alternative method for virus purification because it is
Eskelin, Katri Johanna +5 more
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Nanomaterials, 2020 Among different nanomaterials, gold and silver nanoparticles (AuNPs and AgNPs) have become useful tools for a wide variety of applications in general, and particularly for plasmonic assays.
Lorenzo Sanjuan-Navarro +3 more
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The Power of Field-Flow Fractionation in Characterization of Nanoparticles in Drug Delivery
Molecules, 2023 Asymmetric-flow field-flow fractionation (AF4) is a gentle, flexible, and powerful separation technique that is widely utilized for fractionating nanometer-sized analytes, which extend to many emerging nanocarriers for drug delivery, including lipid ...
Juan Bian +3 more
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Screening for protein–protein interactions with asymmetrical flow field-flow fractionation [PDF]
Journal of Pharmaceutical Sciences, 2021 We describe a new method for screening protein-protein interaction of biopharmaceutical molecules at dilute concentrations to predict development issues at high concentration. The method is based on Asymmetrical Flow Field-Flow Fractionation (AF4) measurements using well known effects of protein-protein attraction on the fractionation profile due to ...
Per-Olof, Wahlund +2 more
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