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Asymmetrical flow field-flow-fractionation in pharmaceutical analytics [PDF]

open access: yes, 2003
Due to enormous progress in recombinant DNA techniques and methodology, a multitude of biosynthetic, pharmaceutically relevant polypeptides and proteins became available in the past decade and have been employed in numerous pharmaceutical products. Concomitantly, substantial progress was made in pharmaceutical formulation development of peptides and ...
Fraunhofer, Wolfgang
openaire   +3 more sources

Analysis of Peptides using Asymmetrical Flow Field-flow Fractionation (AF4)

Journal of Pharmaceutical Sciences, 2021
While asymmetrical flow field-flow fractionation (AF4) has been widely used for separation of high molecular weight species and even particles, its ability to resolve lower molecular weight species has rarely been explored. Over the course of many projects, we have discovered that AF4 can be an effective analytical method for separating peptides from ...
Ryan R, Manning   +6 more
openaire   +2 more sources

Field and flow programming in frit-inlet asymmetrical flow field-flow fractionation

Journal of Chromatography A, 2002
The separation of wide molecular mass (Mr) ranges of macromolecules using frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF) has been improved by implementing a combination of field and flow programming. In this first implementation, field strength (governed by the cross flow-rate through the membrane-covered accumulation wall) is ...
Myeong Hee, Moon   +3 more
openaire   +3 more sources

Asymmetric Flow Field-Flow Fractionation in the Field of Nanomedicine

Analytical Chemistry, 2014
Asymmetric flow field-flow fractionation (AF4) is a widely used and versatile technique in the family of field-flow fractionations, indicated by a rapidly increasing number of publications. It represents a gentle separation and characterization method, where nonspecific interactions are reduced to a minimum, allows a broad separation range from several
Michael, Wagner   +4 more
openaire   +2 more sources

Miniaturization of Frit Inlet Asymmetrical Flow Field-Flow Fractionation

Analytical Chemistry, 2004
A miniaturized frit inlet asymmetrical flow field-flow fractionation (mFI-AFlFFF) channel has been constructed and tested for the separation of proteins. By scaling down the geometrical channel dimension of a conventional FI-AFlFFF system, flow rate ranges that can be manipulated were decreased to 20-30 microL/min, which reduces the injection amount of
Dukjin, Kang, Myeong Hee, Moon
openaire   +2 more sources

Asymmetric Flow Field-Flow Fractionation Investigation of Magnetopolyplexes

Macromolecular Chemistry and Physics, 2015
Asymmetric flow field-flow fractionation (AF4) is an analytical separation technique capable of providing information on different parameters of complex systems in a single measurement. Although it shows promise to be an important tool in the field of gene therapy, AF4 has only occasionally been used to study gene delivery vectors such as polyplexes ...
Haladjova E.   +6 more
openaire   +2 more sources

Fractionation of Humic Substances by Asymmetrical Flow Field-Flow Fractionation

Chromatographia, 2005
Asymmetrical flow field-flow fractionation was used to investigate the effects of pH and ionic strength of the buffer, and the binding of polyaromatic hydrocarbons (PAHs) on the particle sizes of humic substances (HSs). Particle sizes were greater when HSs were present in acidic medium with phosphate buffer than when they were present in alkaline ...
G. Yohannes   +3 more
openaire   +1 more source

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