Results 241 to 250 of about 8,035 (281)
Some of the next articles are maybe not open access.
Miniaturization of Frit Inlet Asymmetrical Flow Field-Flow Fractionation
Analytical Chemistry, 2004A miniaturized frit inlet asymmetrical flow field-flow fractionation (mFI-AFlFFF) channel has been constructed and tested for the separation of proteins. By scaling down the geometrical channel dimension of a conventional FI-AFlFFF system, flow rate ranges that can be manipulated were decreased to 20-30 microL/min, which reduces the injection amount of
Dukjin, Kang, Myeong Hee, Moon
openaire +2 more sources
Asymmetrical flow field-flow fractionation for the analysis of PEG-asparaginase
Talanta, 2016Monomethoxypolyethylene glycol L-asparaginase (PEG-ASNASE) is the PEGylated version of the enzyme L-asparaginase (ASNASE). Both are used for remission induction in acute lymphoblastic leukemia (ALL) and non-Hodgkin's lymphoma (NHL). The treatment control is generally carried out by performing activity assays, though methods to determine the actual ...
C, John +4 more
openaire +2 more sources
Miniaturized asymmetrical flow field-flow fractionation: Application to biological vesicles
Journal of Separation Science, 2007AbstractAsymmetrical flow field‐flow fractionation (AFlFFF) has been carried out in a miniaturized channel by reducing the channel dimensions. Performance of the miniaturized AFlFFF (mAFlFFF) channel was evaluated with standard proteins and polystyrene latex spheres from nanometer to micrometer size.
Dukjin Kang +2 more
exaly +3 more sources
Fractionation of Prion Protein Aggregates by Asymmetrical Flow Field‐Flow Fractionation
2006Achieving the successful separation and analysis of amyloid and other large protein aggregates can be a difficult proposition. Field-flow fractionation (FFF) is a flow-based separation method like chromatography; however, FFF is capable of high-resolution separations in the absence of a stationary matrix.
Jay R, Silveira +2 more
openaire +2 more sources
Analysis of Peptides using Asymmetrical Flow Field-flow Fractionation (AF4)
Journal of Pharmaceutical Sciences, 2021While asymmetrical flow field-flow fractionation (AF4) has been widely used for separation of high molecular weight species and even particles, its ability to resolve lower molecular weight species has rarely been explored. Over the course of many projects, we have discovered that AF4 can be an effective analytical method for separating peptides from ...
Ryan R, Manning +6 more
openaire +2 more sources
Asymmetric Flow Field-Flow Fractionation Investigation of Magnetopolyplexes
Macromolecular Chemistry and Physics, 2015Asymmetric flow field-flow fractionation (AF4) is an analytical separation technique capable of providing information on different parameters of complex systems in a single measurement. Although it shows promise to be an important tool in the field of gene therapy, AF4 has only occasionally been used to study gene delivery vectors such as polyplexes ...
Haladjova E. +6 more
openaire +2 more sources
Flow optimisations with increased channel thickness in asymmetrical flow field-flow fractionation
Journal of Chromatography A, 2018Retention in flow field-flow fractionation (flow FFF) is generally governed by the combination of crossflow and migration flowrates. Especially for an asymmetrical flow FFF (AF4) channel in which the channel-inlet flow is divided into crossflow and outflow, the separation of low-molecular-weight proteins or macromolecules requires a relatively high ...
Joon Seon Yang, Myeong Hee Moon
openaire +2 more sources
Journal of Chromatography A, 2021
The steadily rising interest in the investigation of interactions between nanomaterials and biological media has also led to an increasing interest in asymmetrical flow field-flow fractionation (AF-FFF). The biggest strength of AF-FFF is the possibility to alter the flow profiles to suit a specific separation problem. In this paper, the influence of an
Martin Berger +6 more
openaire +2 more sources
The steadily rising interest in the investigation of interactions between nanomaterials and biological media has also led to an increasing interest in asymmetrical flow field-flow fractionation (AF-FFF). The biggest strength of AF-FFF is the possibility to alter the flow profiles to suit a specific separation problem. In this paper, the influence of an
Martin Berger +6 more
openaire +2 more sources
Circular Asymmetrical Flow Field-Flow Fractionation for the Semipreparative Separation of Particles
Analytical Chemistry, 2003A new technique for the separation and characterization of particles and polymers based on asymmetrical flow field-flow fractionation was developed. The new circular asymmetrical flow field-flow fractionation instrument (CAFFFE) resembles a quasi-parallel arrangement of 12 individual flow channels.
Michael, Maskos, Wolfgang, Schupp
openaire +2 more sources
Optimization and evaluation of asymmetric flow field-flow fractionation of silver nanoparticles
Journal of Chromatography A, 2013Asymmetric flow field-flow fractionation (AF(4)) in combination with on-line optical detection and mass spectrometry is one of the most promising methods for separation and quantification of nanoparticles (NPs) in complex matrices including food. However, to obtain meaningful results regarding especially the NP size distribution a number of parameters ...
Katrin Loeschner +7 more
openaire +5 more sources