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Field and flow programming in frit-inlet asymmetrical flow field-flow fractionation
Journal of Chromatography A, 2002The separation of wide molecular mass (Mr) ranges of macromolecules using frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF) has been improved by implementing a combination of field and flow programming. In this first implementation, field strength (governed by the cross flow-rate through the membrane-covered accumulation wall) is ...
Myeong Hee Moon +2 more
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Asymmetrical flow field-flow-fractionation in pharmaceutical analytics [PDF]
, 2003 Due to enormous progress in recombinant DNA techniques and methodology, a multitude of biosynthetic, pharmaceutically relevant polypeptides and proteins became available in the past decade and have been employed in numerous pharmaceutical products. Concomitantly, substantial progress was made in pharmaceutical formulation development of peptides and ...
Fraunhofer, Wolfgang
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Miniaturized asymmetrical flow field-flow fractionation: Application to biological vesicles
Journal of Separation Science, 2007AbstractAsymmetrical flow field‐flow fractionation (AFlFFF) has been carried out in a miniaturized channel by reducing the channel dimensions. Performance of the miniaturized AFlFFF (mAFlFFF) channel was evaluated with standard proteins and polystyrene latex spheres from nanometer to micrometer size.
Dukjin Kang +2 more
exaly +3 more sources
Optimization of asymmetrical flow field-flow fractionation (AF4)
LC GC Europe, 2010 Asymmetrical flow field-flow fractionation (AF4) can be used on a routine basis to separate high-molecular-weight compounds as an alternative to size-exclusion chromatography (SEC), but the principle of separation is very different and a different approach is required for optimization.
Qureshi, R.N., Kok, W.T.
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Polymer, 2014 Asymmetrical flow field flow fractionation (AF4), when coupled with multi-angle laser light scattering (MALLS), is a very powerful technique for determination of the macromolecular structure of high molar mass (branched) polysaccharides.
Agnes Rolland-Sabaté, Katja Loos
exaly +2 more sources
Asymmetric Flow Field-Flow Fractionation in the Field of Nanomedicine
Analytical Chemistry, 2014Asymmetric flow field-flow fractionation (AF4) is a widely used and versatile technique in the family of field-flow fractionations, indicated by a rapidly increasing number of publications. It represents a gentle separation and characterization method, where nonspecific interactions are reduced to a minimum, allows a broad separation range from several
Michael, Wagner +4 more
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Water Science and Technology: Water Supply, 2017 Asymmetrical flow field-flow fractionation (AF4) has been used to characterise the size and organic carbon concentration of colloids in environmental samples, without extensive pre-treatment. The addition of a true online organic carbon detector (OCD) to
Thomas Meyn
exaly +2 more sources
Fractionation of Humic Substances by Asymmetrical Flow Field-Flow Fractionation
Chromatographia, 2005Asymmetrical flow field-flow fractionation was used to investigate the effects of pH and ionic strength of the buffer, and the binding of polyaromatic hydrocarbons (PAHs) on the particle sizes of humic substances (HSs). Particle sizes were greater when HSs were present in acidic medium with phosphate buffer than when they were present in alkaline ...
G. Yohannes +3 more
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Analysis of plant ribosomes with asymmetric flow field-flow fractionation
Analytical and Bioanalytical Chemistry, 2013Ribosome profiling is a technique used to separate ribosomal subunits, 80S ribosomes (monosomes), and polyribosomes (polysomes) from other RNA-protein complexes. It is traditionally performed in sucrose gradients. In this study, we used asymmetric flow field-flow fractionation (AsFlFFF) to characterize ribosome profiles of Nicotiana benthamiana plants.
Eskelin Katri +2 more
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The use of asymmetrical flow field-flow fractionation in pharmaceutics and biopharmaceutics
European Journal of Pharmaceutics and Biopharmaceutics, 2004Field-flow fractionation (FFF) is a family of flexible analytical fractionating techniques which have the advantage that the separation of analytes is achieved, solely through the interaction of the sample with an external, perpendicular physical field, rather than by the interaction with a stationary phase.
Wolfgang, Fraunhofer, Gerhard, Winter
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