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Analysis of plant ribosomes with asymmetric flow field-flow fractionation
Analytical and Bioanalytical Chemistry, 2013Ribosome profiling is a technique used to separate ribosomal subunits, 80S ribosomes (monosomes), and polyribosomes (polysomes) from other RNA-protein complexes. It is traditionally performed in sucrose gradients. In this study, we used asymmetric flow field-flow fractionation (AsFlFFF) to characterize ribosome profiles of Nicotiana benthamiana plants.
Eskelin Katri +2 more
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Distinguishing nanoparticle drug release mechanisms by asymmetric flow field–flow fractionation
Journal of Controlled Release, 2022This research demonstrates the development, application, and mechanistic value of a multi-detector asymmetric flow field-flow fractionation (AF4) approach to acquire size-resolved drug loading and release profiles from polymeric nanoparticles (NPs). AF4 was hyphenated with multiple online detectors, including dynamic and multi-angle light scattering ...
Sheyda, Shakiba +8 more
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Miniaturized asymmetrical flow field‐flow fractionation: Application to biological vesicles
Journal of Separation Science, 2007AbstractAsymmetrical flow field‐flow fractionation (AFlFFF) has been carried out in a miniaturized channel by reducing the channel dimensions. Performance of the miniaturized AFlFFF (mAFlFFF) channel was evaluated with standard proteins and polystyrene latex spheres from nanometer to micrometer size.
Oh, Sunok. +5 more
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Fractionation of Prion Protein Aggregates by Asymmetrical Flow Field‐Flow Fractionation
2006Achieving the successful separation and analysis of amyloid and other large protein aggregates can be a difficult proposition. Field-flow fractionation (FFF) is a flow-based separation method like chromatography; however, FFF is capable of high-resolution separations in the absence of a stationary matrix.
Jay R, Silveira +2 more
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Aptamer–protein binding detected by asymmetric flow field flow fractionation
Journal of Chromatography A, 2013Asymmetric flow field flow fractionation (AF4) should be suitable for the study of aptamer-target binding, because its gentle separation would impose little disturbance to the complex structure, and it can use carrier solutions with high salt concentrations to provide the most optimal interaction environment to the complex.
Samantha, Schachermeyer +2 more
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Flow optimisations with increased channel thickness in asymmetrical flow field-flow fractionation
Journal of Chromatography A, 2018Retention in flow field-flow fractionation (flow FFF) is generally governed by the combination of crossflow and migration flowrates. Especially for an asymmetrical flow FFF (AF4) channel in which the channel-inlet flow is divided into crossflow and outflow, the separation of low-molecular-weight proteins or macromolecules requires a relatively high ...
Joon Seon Yang, Myeong Hee Moon
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Asymmetrical flow field-flow fractionation of white wine chromophoric colloidal matter
Analytical and Bioanalytical Chemistry, 2017Two analytical separation methods-size-exclusion chromatography and asymmetrical flow field-flow fractionation-were implemented to evaluate the integrity of the colloidal composition of Chardonnay white wine and the impact of pressing and fermentations on the final macromolecular composition.
Coelho, Christian +6 more
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Present situation and development trends of asymmetrical flow field-flow fractionation
Chinese Journal of Chromatography, 2017Field-flow fractionation (FFF) is a kind of mature separation technologies in the field of bioanalysis, feasible of separating analytes with the differences of certain physical and chemical properties by the combination effects of two orthogonal force fields (flow field and external force field).
Qihui, Liang +3 more
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Optimization of asymmetrical flow field-flow fractionation
LC GC North America, 2011Asymmetrical flow field-flow fractionation can be used on a routine basis to separate high molecular weight compounds as an alternative to size-exclusion chromatography (SEC), but the principle of separation is very different and a different approach is required for optimization.
Qureshi, R.N., Kok, W.T.
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Asymmetric flow field-flow fractionation of superferrimagnetic iron oxide multicore nanoparticles
Nanotechnology, 2012Magnetic nanoparticles are very useful for various medical applications where each application requires particles with specific magnetic properties. In this paper we describe the modification of the magnetic properties of magnetic multicore nanoparticles (MCNPs) by size dependent fractionation. This classification was carried out by means of asymmetric
Dutz, Silvio +4 more
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