Results 21 to 30 of about 7,963 (285)

Asymmetrical flow field-flow fractionation in purification of an enveloped bacteriophage ϕ6 [PDF]

Journal of Chromatography B, 2018
Basic and applied virus research requires specimens that are purified to high homogeneity. Thus, there is much interest in the efficient production and purification of viruses and their subassemblies. Advances in the production steps have shifted the bottle neck of the process to the purification. Nonetheless, the development of purification techniques
Lampi, Mirka, Oksanen, Hanna M., Meier, Florian, Moldenhauer, Evelin, Poranen, Minna M., Bamford, Dennis H., Eskelin, Katri   +6 more
openaire   +5 more sources

AF4-to-SAXS: expanded characterization of nanoparticles and proteins at the P12 BioSAXS beamline [PDF]

Journal of Synchrotron Radiation
Biological small-angle X-ray scattering (SAXS) is a versatile and powerful technique for investigating the structural and biophysical properties of biologically and pharmaceutically relevant macromolecules and nanoparticles. SAXS offers detailed insights
Stefano Da Vela, Kim Bartels, Daniel Franke, Dymtro Soloviov, Tobias Gräwert, Dmitry Molodenskiy, Bastian Kolb, Christoph Wilhelmy, Roland Drexel, Florian Meier, Heinrich Haas, Peter Langguth, Melissa A. Graewert   +12 more
doaj   +2 more sources

Electrical asymmetrical flow field-flow fractionation: Fundamentals, evolution, applications, and prospects [PDF]

Journal of Chromatography A
Among the plethora of techniques that conforms the Field-Flow Fractionation (FFF) family, electrical field-flow fractionation (ElFFF) was designed to separate different analytes based on their size and electrophoretic mobility (µ). However, major technical and operational issues made this technique to fall into oblivion.
Armando Sánchez-Cachero, Andrea López-Gutiérrez, Nuria Rodríguez Fariñas, Francisco Javier Guzmán Bernardo, Ángel Ríos, Rosa Carmen Rodríguez Martín-Doimeadios   +5 more
openaire   +5 more sources

Asymmetric flow field‐flow fractionation of liposomes: optimization of fractionation variables [PDF]

Journal of Separation Science, 2009
AbstractThe purpose of this study was to investigate the influence of ionic strength of the carrier liquid, cross flow rate, focus flow rate, and sample load on the retention behavior of liposomes in asymmetric flow field‐flow fractionation (AF4). Two differently prepared samples of large unilamellar vesicles (LUV) were used. Experiments were performed
Stefan, Hupfeld, Dominik, Ausbacher, Martin, Brandl   +2 more
openaire   +3 more sources

Revisiting the dynamics of proteins during milk powder hydration using asymmetric flow field-flow fractionation (AF4)

Current Research in Food Science, 2021
The dynamics of β-casein and casein micelles in the reconstitution of skim milk were revisited in this study. β-casein migrates into casein micelles upon an increase in temperatures due to an increase in the hydrophobic effect and lower calcium-phosphate
Anouk Lie-Piang, Mats Leeman, Alejandra Castro, Erik Börjesson, Lars Nilsson   +4 more
doaj   +1 more source

Asymmetric flow field flow fractionation methods for virus purification [PDF]

Journal of Chromatography A, 2016
Detailed biochemical and biophysical characterization of viruses requires viral preparations of high quantity and purity. The optimization of virus production and purification is an essential, but laborious and time-consuming process. Asymmetric flow field flow fractionation (AF4) is an attractive alternative method for virus purification because it is
Eskelin, Katri Johanna, Lampi, Mirka, Meier, Florian, Moldenhauer, Evelin, Bamford, Dennis Henry, Oksanen, Hanna Maarit   +5 more
openaire   +3 more sources

Aqueous Dilution of Noble NPs Bulk Dispersions: Modeling Instability due to Dissolution by AF4 and Stablishing Considerations for Plasmonic Assays

Nanomaterials, 2020
Among different nanomaterials, gold and silver nanoparticles (AuNPs and AgNPs) have become useful tools for a wide variety of applications in general, and particularly for plasmonic assays.
Lorenzo Sanjuan-Navarro, Aaron Boughbina-Portolés, Yolanda Moliner-Martínez, Pilar Campíns-Falcó   +3 more
doaj   +1 more source

The Power of Field-Flow Fractionation in Characterization of Nanoparticles in Drug Delivery

Molecules, 2023
Asymmetric-flow field-flow fractionation (AF4) is a gentle, flexible, and powerful separation technique that is widely utilized for fractionating nanometer-sized analytes, which extend to many emerging nanocarriers for drug delivery, including lipid ...
Juan Bian, Nemal Gobalasingham, Anatolii Purchel, Jessica Lin   +3 more
doaj   +1 more source

Screening for protein–protein interactions with asymmetrical flow field-flow fractionation [PDF]

Journal of Pharmaceutical Sciences, 2021
We describe a new method for screening protein-protein interaction of biopharmaceutical molecules at dilute concentrations to predict development issues at high concentration. The method is based on Asymmetrical Flow Field-Flow Fractionation (AF4) measurements using well known effects of protein-protein attraction on the fractionation profile due to ...
Per-Olof, Wahlund, Nikolai, Lorenzen, Christian, Rischel   +2 more
openaire   +2 more sources

Field-Flow Fractionation in Molecular Biology and Biotechnology

Molecules, 2023
Field-flow fractionation (FFF) is a family of single-phase separative techniques exploited to gently separate and characterize nano- and microsystems in suspension.
Stefano Giordani, Valentina Marassi, Anna Placci, Andrea Zattoni, Barbara Roda, Pierluigi Reschiglian   +5 more
doaj   +1 more source